TU Chang,KE Wenshan,ZOU Lulu,et al.Screening and Identification of Walnut Bacterial Blight[J].Northern Horticulture,2020,44(23):10-16.[doi:10.11937/bfyy.20200670]
核桃细菌性疫病的生防菌筛选与鉴定
- Title:
- Screening and Identification of Walnut Bacterial Blight
- 文献标志码:
- A
- 摘要:
- 以枇杷、茄子和水杉等45种植物的健康叶片或茎中分离得到的80株植物内生菌为试材，采用平板对峙法，研究了这些菌株对病原菌树生黄单胞菌核桃致病变种 Xanthomonas arboricola pv.juglandis的影响，以期为核桃细菌性疫病的生物防治提供理论参考依据。结果表明：得到5株有明显拮抗作用的菌株，分别是PPE17-18、QCE17-2、QZYE-41、QZJ-42和SSE17-24。其中菌株QZYE-41抑菌能力最强，PPE17-18和SSE17-24的抑菌能力较弱，5株菌株均为革兰氏阳性菌。通过16S rDNA和gyrB基因测序分析，结合菌落形态和生理生化测试，初步确定QZYE-41和SSE17-24为枯草芽孢杆菌(Bacillus subtilis)，PPE17-18和QZJ-42为鲍曼不动杆菌(Acinetobacter baumannii),QCE17-2为不动杆菌属(Acinetobacter sp.)。该研究为核桃细菌性疫病的生物防治菌的开发应用提供了潜在生物新材料。
- Abstract:
- In this study,including loquat,eggplant and metasequoia,45 kinds plants healthy leaves or stems were used as experiment materials,80 strains of endophytic bacteria were isolated.By using plate-confrontation assay,the interactions were studied between the isolated strains and the walnut pathogenic bacterium Xanthomonas arboricola pv.juglandis,respectively,in order to provide theoretical basis for the biological control of walnut bacterial blight.The results showed that five of the isolated,PPE17-18,QCE17-2,QZYE-41,QZJ-42 and SSE17-24,had obvious antagonistic effects.Among them,QZYE-41 had the strongest antibacterial ability,while PPE17-18 and SSE17-24 had weaker antibacterial ability.All the five strains were gram-positive bacteria.Through 16S rDNA and gyrB gene sequencing analysis,combined with colony morphology and physiological and biochemical tests,it was preliminarily determined that both QZYE-41 and SSE17-24 were Bacillus subtilis,PPE17-18 and QZJ-42 were Acinetobacter baumannii,and QCE17-2 was Acinetobacter sp.This study provided a potential new material for the developing and application for biological control of walnut bacterial blight.
参考文献/References:
[1]孙完清.浅析提高核桃产量的技术要点[J].种子科技,2017,35(11):61-62.[2]宫永红.核桃细菌性黑斑病研究进展[J].北方果树,2012(6):1-4.[3]马荣,王阳阳,刘晓琳,等.新疆核桃树腐烂病拮抗细菌的筛选及初步鉴定[J].新疆农业科学,2015,52(5):895-901.[4]张晶晶,李建贵,郭艺鹏,等.新疆核桃腐烂病拮抗菌的筛选及鉴定[J].经济林研究,2016,34(1):71-75.[5]黄海婵,裘娟萍.枯草芽孢杆菌防治植物病害的研究进展[J].浙江农业科学,2005(3):213-215.[6]陈成,方银,金超,等.枯草芽孢杆菌对桑葚采后致腐微生物的抑菌作用[J].蚕业科学,2016,42(1):118-123.[7]周翠,乔鲁芹,金静,等.一株枯草芽孢杆菌挥发性物质的抑菌作用初步研究[J].农药学学报,2011,13(2):201-204.[8]缪应纯,徐晓林,缪应举,等.鲍曼不动杆菌研究进展[J].山东化工,2017,23(46):49-51.[9]袁梦思,李建文,刘梦华,等.1株不动杆菌发酵液对水稻纹枯病菌的抑菌作用[J].江苏农业科学,2016,44(2):164-165.[10]蔺经,杨青松,李晓刚.一株不动杆菌次生代谢活性产物的抑菌作用及对果树病害的防治效果[J].江西农业学报,2010,22(10):78-79.[11]于秋香,李扬,刘勇,等.核桃细菌性黑斑病发病规律及防治措施[J].河北果树,2017(4):27.[12]BIRD L S,LIVERMAN P,PEROY R G,et al.Evidence that microor-ganisms in and on tissues have a role in a mechanism of multiad-versity resistance in cotton[C].Memphis TN:Proc Beltwide Cotton Prod ResConf,National Cotton Council of America,1980.[13]ELZIK K M.Symbiotic organism associated with plant part of MAR and no-MAR cottons[J].Phytopathology,1985,75(11):1344-1347.[14]SCHIPPER B.Molecular aspects of plant growth affecting pseud-omonas species in reorganization in micro plant symbiotic and pathogen interaction[M].Berlin Heidelbery:Spring-Verlay,1986.[15]COOK C G.Effect of treatment with Bacillus species on cottonroot traits,yield,and phymatotrichum root rot[J].Idem,1987,47(3):235-239.[16]FU B,CHEN Q,WEI M,et al.Complete genome sequence of Xanthomonas arboricola pv. juglandis strain DW3F3,isolated from a Juglans regia L.bacterial blighted fruitlet[J].Genome Announc,2018,6:e00023-18.[17]李娟,夏延斌,蔡文韬.辣椒内生菌的分离及拮抗菌的筛选与鉴定[J].农产品加工学刊,2013(1):1-3.[18]刘胜贵,周玉莎,易江,等.柑橘内生菌的分离、鉴定及其对沙皮病的防治作用[J].生命科学研究,2016,20(5):429-434.[19]王志勇,刘秀娟.植物内生菌分离方法的研究现状[J].贵州农业科学,2014,42(1):152-155.[20]关小敏,孟品品,刘星,等.马铃薯黑痣病生防菌的筛选和鉴定及其生长条件的研究[J].干旱地区农业研究,2015,33(3):90-95.[21]中国科学院微生物所细菌分类组.一般细菌常用鉴定方法[M].北京:科学出版社,1987.[22]东秀珠,蔡妙英.常见细菌鉴定手册[M].北京:科学出版社,2001.[23]周德庆.微生物学实验手册[M].上海:科学技术出版社,1986.[24]冯思玲.系统发育树构建方法研究[J].信息技术,2009(6):38-44.
相似文献/References:
[1]孙正祥,王丰,张昊,等.内生菌SZ-1的定殖力及其对姜瘟病的防效[J].北方园艺,2013,37(01):134.
SUN Zheng-xiang,WANG Feng,ZHANG Hao,et al.Colonization Ability and Effect on Ginger Bacterial Wilt of Endophyte SZ-1[J].Northern Horticulture,2013,37(23):134.
[2]金玉,任梦楠,杨红澎,等.短柄龙胆内生菌的分离鉴定及抗菌活性分析[J].北方园艺,2018,42(01):135.[doi:10.11937/bfyy.20171784]
JIN Yu,REN Mengnan,YANG Hongpeng,et al.Isolation,Identification and Antibiotic Activities of Endophytes in Gentiana stipitata[J].Northern Horticulture,2018,42(23):135.[doi:10.11937/bfyy.20171784]
[3]王夏,刘金平,孙菲菲.内生菌肥料对设施西瓜生长的影响[J].北方园艺,2019,43(04):80.[doi:10.11937/bfyy.20182538]
WANG Xia,LIU Jinping,SUN Feifei.Effects of Endophytes Fertilizer on Growth of Watermelon in Facilities[J].Northern Horticulture,2019,43(23):80.[doi:10.11937/bfyy.20182538]
[4]刘月芹,罗丽媛,刘欣瑞,等.林下种植灵芝内生细菌和放线菌的分离鉴定[J].北方园艺,2023,(03):120.[doi:10.11937/bfyy.20222032]
LIU Yueqin,LUO Liyuan,LIU Xinrui,et al.Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum[J].Northern Horticulture,2023,(23):120.[doi:10.11937/bfyy.20222032]
[5]李铂,高晓敏,王楠,等.不同产地沙棘种子内生菌群落组成与多样性分析[J].北方园艺,2024,(8):94.[doi:10.11937/bfyy.20233384]
LI Bo,GAO Xiaomin,WANG Nan,et al.Community Composition and Diversity Analysis of Endophytes in Hippophae rhamnoides Seeds From Different Locations[J].Northern Horticulture,2024,(23):94.[doi:10.11937/bfyy.20233384]
备注/Memo
第一作者简介：涂昌（1995-），男，硕士研究生，研究方向为植物生态学。E-mail：420033946@qq.com.责任作者：王立华（1963-），男，博士，教授，现主要从事植物保护教学与科研等工作。E-mail：lwang@hbeu.edu.cn.基金项目：2019年中央引导地方科技发展专项资助项目（2019ZYYD045）。收稿日期：2020-02-27