WANG Huan,LOU Ling,BU Hongshi,et al.Liquid Fermentation Technology of Marasmius epiphyllus[J].Northern Horticulture,2020,44(22):127-131.[doi:10.11937/bfyy.20200646]
叶生小皮伞的液体发酵工艺优化
- Title:
- Liquid Fermentation Technology of Marasmius epiphyllus
- Keywords:
- Marasmius epiphyllus; submerged fermentation; orthogonal experiment; mycelial biomass; fermentation conditions
- 文献标志码:
- A
- 摘要:
- 以叶生小皮伞为试材，采用单因素和L9（34）正交实验，以菌丝生物量为考察指标，优化叶生小皮伞ME-1菌株的液体发酵高产菌丝体的培养基配方和液体发酵条件。以期提高ME-1的菌丝体收率，为后续固体发酵及对发酵产物的质量评价等研究提供参考依据。结果表明：最佳培养基配方为3%麦芽糖、2%酵母粉、0.15%KH2PO4、0.1%MgSO4·7H2O；发酵条件为在温度为（27±1）℃，转速为150 r·min-1时，适宜pH为5～6，最佳装量为140 mL·（500mL）-1。在该发酵工艺下，菌丝干质量可达1.472 g·（100mL）-1。
- Abstract:
- The purpose of this study was to optimize fermentation conditions for Marasmius epiphyllus，the wild strain ME-1 of Marasmius epiphyllu was investigated by using single factor experiment and orthogonal experiment，mycelial biomass was chosen for main index of evaluation to optimize fermentation broth and liquid fermentation conditions.The results showed that the optimal medium formula consists of 3% maltose，2% yeast powder，0.15% KH2PO4，0.1% MgSO4·7H2O，the fermentation conditions were as follows：fermentation temperature of (27±1)℃，rotary speed of 150 r·min-1，the initial pH of 5-6，fluid volume of 140 mL·（500mL）-1，the mycelial biomass reached 1.472 g·（100mL）-1 under this fermentation condition.In this study，the yield of the mycelium ME-1 was increased，which can provide a theoretical basis for further studies on solid fermentation and quality evaluation of fermentation products.
参考文献/References:
[1]FRIES E M.Epicrisis systematis mycologici，seu synopsis hymeno mycetum.i-xii[M].Uppsala：Typographia Academica，1838.[2]李玉，李泰辉，杨祝良，等.中国大型菌物资源图鉴[M].郑州：中原农民出版社，2015.[3]GAO Y，LIU D，YANG X L，et al. Isolation and characterization of glycopeptides with analgesic activities from Marasmius androsaceus[J].European Journal of Integrative Medicine，2014，6(6)：728.[4]丁艳如，张晶晶，胡颖军，等.安络小皮伞菌液体发酵条件的优化[J].河南大学学报（自然科学版），2015，45(6)：712-713.[5]SONG J，XING G Y，CAO J M，et al.Investigation of the antidepressant effects of exopolysaccharides obtained from Marasmius androsaceus fermentation in a mouse model[J].Molecular Medicine Reports，2016，13(1)：939-946.[6]图力古尔，包海鹰，李玉，等.吉林省担子菌补记（三）[J].吉林农业大学学报，2001，23(4)：37-40.[7]吴兴亮，连宾，邹芳伦，等.贵州大沙河自然保护区大型真菌资源生态特征[J].贵州科学，2005，23(1)：1-12.[8]娄玲，穆双双，胡德，等.叶生小皮伞菌的生物学特性研究[J].中国食用菌，2018，37(6)：50-55，63.[9]张医芝.灰树花菌丝体液体深层发酵工艺优化及降血糖和抗肿瘤活性研究[D].长春：吉林大学，2017.
相似文献/References:
[1]周会明,张焱珍,陈银辉,等.荷叶离褶伞液体发酵菌株筛选研究[J].北方园艺,2014,38(03):134.
ZHOU Hui-ming,ZHANG Yan-zhen,CHEN Yin-hui,et al.Study on Screening of Liquid Fermentation Strains of Lyophyllum decastes[J].Northern Horticulture,2014,38(22):134.
[2]暴增海,贾超艳,江倩倩,等.真姬菇、鸡汁菇和蛹虫草培养特性的观察与生长量的测定[J].北方园艺,2012,36(23):162.
BAO Zeng-hai,JIA Chao-yan,JIANG Qian-qian,et al.Cultivation Characteristics and Growth of Hypsizygus marmoreus，Jizhigu and Cordyceps militaris[J].Northern Horticulture,2012,36(22):162.
[3]李宇伟,连瑞丽,班强,等.灵芝液体发酵培养条件的响应面法优化研究[J].北方园艺,2012,36(13):179.
LI Yu-wei,LIAN Rui-li,BAN Qiang,et al.Optimization of Liquid Fermentation Conditions for Ganoderma Lucidum Using Response Surface Methodology[J].Northern Horticulture,2012,36(22):179.
[4]李海源,张亮,张美萍,等.日本灵芝灵芝酸发酵工艺的优化[J].北方园艺,2012,36(12):170.
LIHai-yuan,ZHANGLiang,ZHANGMei-ping,et al.OptimizationofFermentationTechnologyforGanodermaAcidofJapaneseGanoderma[J].Northern Horticulture,2012,36(22):170.
[5]刘青青,戴 玄,陈今朝.羊肚菌液体发酵研究进展[J].北方园艺,2015,39(01):190.[doi:10.11937/bfyy.201501051]
LIU Qing-qing,DAI Xuan,CHEN Jin-zhao.Research Progress on Liquid Fermentation of Morchella esculenta L.Pars[J].Northern Horticulture,2015,39(22):190.[doi:10.11937/bfyy.201501051]
[6]乔艳明,陈文强,邓百万,等.秦巴山区香菇胞外多糖高产菌株的筛选[J].北方园艺,2015,39(08):137.[doi:10.11937/bfyy.201508036]
QIAO Yan-ming,CHEN Wen-qiang,DENG Bai-wan,et al.Screening of Lentinula edodes with High Exopolysaccharide inQinling-Bashan Mountainous Area[J].Northern Horticulture,2015,39(22):137.[doi:10.11937/bfyy.201508036]
[7]魏雅冬,王广慧,郭海滨,等.香菇液体发酵培养基的优化研究[J].北方园艺,2015,39(12):136.[doi:10.11937/bfyy.201512035]
WEI Ya-dong,WANG Guang-hui,GUO Hai-bin,et al.Study on Optimization of Mushrooms Liquid Fermentation Medium[J].Northern Horticulture,2015,39(22):136.[doi:10.11937/bfyy.201512035]
[8]王 广 耀,董 立 华,张红.液体羊肚菌浅层发酵培养基的优化研究[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007068]
W ANG Guang -yao,DONG L- i hua,ZHANG Hong.The Study of O ptimization Conditions about Fermentation Medium Shallow of Morchella[J].Northern Horticulture,2010,34(22):0.[doi:10.11937/bfyy.201007068]
[9]殷书平,程贤利,陈文强.香菇液体发酵过程中生理指标的检测分析[J].北方园艺,2016,40(08):142.[doi:10.11937/bfyy.201608039]
YIN Shuping,CHENG Xianli,CHEN Wenqiang.Detection and Analysis of Physiological Indexes of Lentinula edodes in Liquid Fermentation Process[J].Northern Horticulture,2016,40(22):142.[doi:10.11937/bfyy.201608039]
[10]张璐,马沁沁,袁向华,等.金地灵芝液体发酵产胞外多糖的营养条件优化[J].北方园艺,2016,40(09):133.[doi:10.11937/bfyy.201609035]
ZHANG Lu,MA Qinqin,YUAN Xianghua,et al.Optimization of Liquid Fermentation of Ganoderma lucidum for Extracellular Polysaccharide Production[J].Northern Horticulture,2016,40(22):133.[doi:10.11937/bfyy.201609035]
备注/Memo
第一作者简介：王欢（1987-），女，博士，助理研究员，现主要从事大型真菌资源开发与利用等研究工作。E-mail：wanghuanmyco@163.com.责任作者：王淑敏（1965-），女，博士，教授，博士生导师，现主要从事中药生药学、微生物与生化药学等研究工作。E-mail：wangsm@ccucm.edu.cn.基金项目：吉林省自然科学基金主题引导资助项目（20190201297JC）；高等学校学科创新引智计划“111”合作基地资助项目（D17014）。收稿日期：2020-02-25