[1]张玮,肖梦婷,黄敏,等.短波紫外线辐照对灰葡萄孢抑菌机理的影响[J].北方园艺,2020,44(18):7-13.[doi:10.11937/bfyy.20200121]
　ZHANG Wei,XIAO Mengting,HUANG Min,et al.Effects of UV-C Irradiation on Antifungal Mechanism of Botrytis cinerea[J].Northern Horticulture,2020,44(18):7-13.[doi:10.11937/bfyy.20200121]
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短波紫外线辐照对灰葡萄孢抑菌机理的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年18 页码: 7-13 栏目: 出版日期: 2020-09-30
Title:
Effects of UV-C Irradiation on Antifungal Mechanism of Botrytis cinerea
作者:
张玮1; 肖梦婷2; 3; 黄敏4; 赵博2; 4
（1.延安大学西安创新学院 土木工程学院，陕西 西安 710100；2.西南科技大学 生命科学与工程学院，四川 绵阳 621010；3.三峡大学 生物与制药学院，湖北 宜昌 443002；4.辐照保藏四川省重点实验室，四川 成都 610101）
Author(s):
ZHANG Wei1; XIAO Mengting2; 3; HUANG Min4; ZHAO Bo2; 4
(1.College of Civil Engineering，Xi′an Innovation College of Yan′an University，Xi′an，Shaanxi 710100；2.School of Life Science and Engineering，Southwest University of Science and Technology，Mianyang，Sichuan 621010；3.College of Biological and Pharmaceutical Sciences，China Three Gorges University，Yichang，Hubei 443002；4.Irradiation Preservation Technology Key Laboratory of Sichuan Province，Chengdu，Sichuan 610101)
关键词:
灰霉病; 短波紫外线; 葡萄果实; 灰葡萄孢; 抑制率
Keywords:
gray mold; short wave ultraviolet; grape fruit; Botrytis cinerea; inhibition ratio
DOI:
10.11937/bfyy.20200121
文献标志码:
A
摘要:
以灰葡萄孢为试材，采用短波紫外线对其进行辐照处理，研究了照射时间为10、20、30、40、50 min的短波紫外线对灰葡萄孢的抑制作用，以期为葡萄灰霉病的防治提供参考依据。结果表明：短波紫外线的照射处理可以显著地抑制灰葡萄孢的生长，其抑制率和照射时间呈正相关，在照射时间为40 min的处理中，灰葡萄孢的生长抑制率达到851%；与对照组相比，紫外照射30 min和40 min处理组在作用2 h以后，灰葡萄孢的相对电导率明显升高；此外，菌丝体自身总糖和可溶性蛋白质含量随着紫外照射时间的延长而逐渐降低，并且紫外线照射处理还会降低灰葡萄孢机体内参与物质能量代谢的相关酶活性，进而干扰其正常萌发与生长，显著降低果实的病斑直径，为葡萄采后无公害防治及保鲜贮藏提供参考指导。
Abstract:
Botrytis cinerea was used as the test fungus to investigate the inhibitory effect of short-wave ultraviolet radiation on it and control effect of irradiation at different times (10，20，30,40，50 minutes).The results showed that the growth of Botrytis cinerea was significantly inhibited by short-wave ultraviolet irradiation，and the inhibition rate was positively correlated with the irradiation time.The growth inhibition rate of Botrytis cinerea reached 851% when treatment time was 40 minutes.Compared with the control group，the relative conductivity of 30 and 40 minutes groups increased significantly after ultraviolet irradiation for 2 hours.The total sugar and soluble protein content of mycelium decreased gradually with the prolonged ultraviolet irradiation time，and ultraviolet irradiation could also reduce the activities of related enzymes involved in substance and energy metabolism in Botrytis cinerea organism，thereby interfering with its normal germination and growth，significantly reducing the diameter of fruit lesions，providing a certain theory and technology guidance for pollution-free control and fresh-keeping storage of grapes after harvest.

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备注/Memo

第一作者简介：张玮（1980-），女，硕士，讲师，现主要从事微生物的教学与科研等工作。E-mail:416049402@qq.com.责任作者：赵博（1979-），男，博士，讲师，现主要从事园艺产品采后生理的教学与科研等工作。E-mail:bozhao@swust.edu.cn.基金项目：四川省科技计划应用基础资助项目（2018JY0531）；辐照保藏四川省重点实验室开放基金资助项目（FZBC2017002）。收稿日期：2020-01-08

更新日期/Last Update: 2020-12-11