[1]魏晓梅,吴丽芳.色素万寿菊雄性不育植株的快速繁殖[J].北方园艺,2021,(02):53-59.[doi:10.11937/bfyy.20200012]
　WEI Xiaomei,WU Lifang.Rapid Propagation for Male Sterile Lines of Pigment Marigold[J].Northern Horticulture,2021,(02):53-59.[doi:10.11937/bfyy.20200012]
点击复制

色素万寿菊雄性不育植株的快速繁殖

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年02 页码: 53-59 栏目: 出版日期: 2021-01-30
Title:
Rapid Propagation for Male Sterile Lines of Pigment Marigold
作者:
魏晓梅; 吴丽芳
（曲靖师范学院 生物资源与食品工程学院，云南高原生物资源保护与利用研究中心，云南 曲靖 655011）
Author(s):
WEI Xiaomei; WU Lifang
(College of Biological Resource and Food Engineering，Qujing Normal University/Center for Yunnan Plateau Biological Resources Protection and Utilization，Qujing，Yunnan 655011)
关键词:
色素万寿菊; 离体快繁; 雄性不育系
Keywords:
pigment marigold; in vitro rapid propagation; male sterile lines
DOI:
10.11937/bfyy.20200012
文献标志码:
A
摘要:
以色素万寿菊为试验材料，采用母本资源不育株叶片、带腋芽茎段和侧芽为外植体，对不同的培养基配方条件下的生长情况进行统计，研究了灭菌处理、生长调节剂组合、AgNO3对丛生芽诱导的影响，以期快速获得遗传稳定的万寿菊雄性不育植株，为杂交育种提供基础研究材料。结果表明：以带腋芽的茎段为最佳外植体，1 g?L-1 HgCl2灭菌10~12 min的灭菌效果最好，B1-3较易诱导丛生芽，其最佳的诱导培养基为MS+1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA+30 g?L-1蔗糖+7 g?L-1琼脂，诱导率高达93%，2.0 mg?L-1 AgNO3有利于丛生芽增殖，使B1-3丛生芽增殖率达151.6%，丛生芽生根培养后移栽成活率达95%以上。发现万寿菊离体培养过程中，外植体不同，其丛生芽诱导率不同，最佳外植体为带腋芽茎段。不同的灭菌剂的灭菌效果不同，1 g?L-1 HgCl2灭菌效果最好；丛生芽诱导时6-BA与NAA不同浓度组合优于6-BA与IAA组合；低浓度AgNO3能够促进丛生芽诱导，高浓度反而会降低。
Abstract:
Pigment marigold was selected as research object.Leaves，stem with axillary buds and lateral buds of sterile plants from pigment marigold mother resources were used to research the effects of sterilization treatment，growth regulator combination and AgNO3 concentration on multiple shoots induction.The aim was to obtain the genetically stable male sterile plants of marigold，and provide basic research materials for cross breeding.The results showed that stem with axillary buds were the best explants.The best sterilization agent was 1 g?L-1 HgCl2 to sterilize 10-12 minutes.B1-3 was easy to induce multiple shoots，MS+1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA+30 g?L-1 sucrose+7 g?L-1 agar was optimal induction medium，the induction rate for B1-3 was up to 93%.2.0 mg?L-1 AgNO3 was beneficial to proliferation of multiple shoots，and the proliferation rate of B1-3 was to 151.6%.After rooting culture，the survival rate of transplanting was over 95%.In vitro culture of marigold，the rate of cluster bud induction was different with different explants.Stem with axillary buds were the best explants.Different sterilization agents had different influenceson sterilization effect，1 g?L-1 HgCl2 of sterilization effect was the best.The combination of 6-BA and NAA was better than that of 6-BA and IAA for cluster buds induction.Low concentration AgNO3 could promote the induction of cluster buds，but high concentrations had opposite effect.

参考文献/References:

[1]DELGADO-VARGAS F,PAREDES-LPEZ O.Effects of enzymatictreatments of marigold flowers on lutein isomeric profiles[J].J Agr Food Chem,1997,45(4):1097-1102.[2]SANTOSA S,JONES P J H.Oxidative stress in ocular disease:Does lutein play a protective role[J].Canadian Medical Association Journal,2005,173(8):861-862.[3]TSAO R,WANG M,DENG Z.Lutein:Separation,antioxidant activity,and potential health benefits[J].ACS Symposium Series,2007,956:352-372.[4]RIEHARD B van B,HARLOLD H S.Continuous flow fast bombardment liquid chromatography/mass spectrometry of earotenoids[J].Analytical Chemistry,1993,65:965-969.[5]李娜,陶承光,王平,等.色素万寿菊试管苗玻璃化现象及防止研究[J].北方园艺,2013(4):108-110.[6]VANEGAS P E,CRUZ-HERNNDEZ A,VALVERDE M E,et al.Plant regeneration via organogenesis in marigold[J].Plant Cell，Tissue and Organ Culture,2002,69:279-283.[7]MOHAMED M A H,HARRIS P J C,HENDERSON J.An efficient in vitro regeneration protocol for Tagetes minuta[J].Plant Cell,Tissue and Organ Culture,1998,5(3):211-215.[8]BENAVIDES M P,CASO O H.Plant regeneration and thiophene formation in tissue cultures of Tagetes mendocina[J].Plant Cell,Tissue and Organ Culture,1993,35(3):211-215.[9]田广红.万寿菊杂交F1代的组织培养与快速繁殖[J].植物生理学通讯,2001,37(6):529-530.[10]苏福才,钱国珍,李巧玲,等.万寿菊茎段组织培养[J].内蒙古农牧学院学报,1999,20(3):108-111.[11]林淦,周建平.快速离体培养万寿菊植株叶片组织[J].江西农业学报,2006,18(3):92-93.[12]赵子刚,曾丽,唐克轩,等.色素万寿菊再生体系的建立及优化[J].园艺学报,2010,37(4):655-666.[13]伍亚平,唐道城.万寿菊雄性不育系离体保存及快繁体系的建立[J].北方园艺,2013(1):116-118.[14]李甫,唐道城,梁顺祥.生长调节剂对万寿菊花药培养的影响[J].青海大学学报(自然科学版),2007,25(1):51-53.[15]张华丽,赵剑颖,张睿鹂,等.万寿菊杂交亲本的离体培养[J].西北植物学报,2011,31(12):2545-2550.[16]胡颂平,刘选明.植物细胞组织培养技术[M].北京:中国农业大学出版社,2014.[17]齐迎春,叶要妹,刘国锋,等.不同基因型孔雀草高效植株再生体系的建立[J].中国农业科学,2005,38(7):1414-1417.[18]KHALID M,CHRAIBI B,ALAIN L.Stimulation of shoot regeneration from cotyledons of Hellianthus annuns by the ethylene inhibitors,silver and cobalt[J].Plant Cell Reports,1991,10:204-207.[19]马光,周波,李玉花.芜菁高频率再生体系的建立及优化[J].园艺学报,2008,35(6):833-840.[20]刘娟旭,刘玲,余义勋,等.车轮梅茎段高效再生体系的建立[J].园艺学报,2008(6):895-898.[21]齐红岩,陈岩,刘雪超.AgNO3对网纹甜瓜试管苗再生和玻璃苗产生的影响[J].植物生理学通讯,2009,45(12):1167-1170.[22]CHI G L,PUA E C,GOH C J.Role of ethylene on shoot regeneration from cotyldeonary expalnts of Brassica campestris ssp.Pekinensis (Lour) Olsson in vitro[J].Plant Physiol,1991,96:178-183.

相似文献/References:

[1]李娜,陶承光,王平,等.色素万寿菊试管苗玻璃化现象及防止研究[J].北方园艺,2013,37(04):108.
[2]夏文娟.色素万寿菊引种研究初探[J].北方园艺,2013,37(07):80.
　XIA Wen-juan.Preliminary Research on Introduction of Pigment Marigold[J].Northern Horticulture,2013,37(02):80.
[3]宁静,赵和祥,蕫然,等.白藓优良单株的选择及离体快繁技术研究[J].北方园艺,2013,37(07):131.
　NING Jing,ZHAO He-xiang,DONG Ran,et al.Study on Selection and Propagation of Superior Individuals of Dictamnus dasycarps Turcz.[J].Northern Horticulture,2013,37(02):131.
[4]张莹,陶佩琳,高政平.三七景天离体快繁技术研究[J].北方园艺,2013,37(07):134.
　ZHANG Ying,TAO Pei-lin,GAO Zheng-ping.Studies on Rapid Propagation in vitro of Sedum aizoon[J].Northern Horticulture,2013,37(02):134.
[5]吴志刚,王平,张玉静,等.“易丰收”植物生长调节剂在色素万寿菊上的应用效果[J].北方园艺,2012,36(16):60.
　WU Zhi-gang,WANG Ping,ZHANG Yu-jing,et al.Study on the Application Effect of ‘EFS’ Plant Growth Regulators on Pigment Marigold[J].Northern Horticulture,2012,36(02):60.
[6]叶飞,建德锋.铁线蕨孢子离体繁殖技术研究[J].北方园艺,2012,36(17):122.
　YE Fei,JIAN De-feng.Study on the Spores Propagation of Adiantum capillus-veneris in vitro[J].Northern Horticulture,2012,36(02):122.
[7]李金英,赵春莉,张志东.黑穗醋栗‘寒丰’离体快繁影响因素研究[J].北方园艺,2012,36(22):119.
　LI Jin-ying,ZHAO Chun-li,ZHANG Zhi-dong.Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.‘Hanfeng’[J].Northern Horticulture,2012,36(02):119.
[8]贾兰虹,张雪岩,吕桂菊.色素万寿菊开花光周期调控规避病害增产效果研究[J].北方园艺,2014,38(17):83.
　JIA Lan-hong,ZHANG Xue-yan,LYU Gui-ju.Study on Control of Photoperiod in Bloom of Pigment Marigold on Avoid Disease and Increase Production[J].Northern Horticulture,2014,38(02):83.
[9]文 　 峰.色素万寿菊杂交种‘ 通菊一号’ 高产栽培技术[J].北方园艺,2010,34(17):0.[doi:10.11937/bfyy.201017043]
　[J].Northern Horticulture,2010,34(02):0.[doi:10.11937/bfyy.201017043]
[10]王欢,许士钊,李旭.长白忍冬的组织培养与快速繁殖[J].北方园艺,2016,40(13):107.[doi:10.11937/bfyy.201613028]
　WANG Huan,XU Shizhao,LI Xu.Tissue Culture and Rapid Propagation of Lonicera ruprechtiana Regel[J].Northern Horticulture,2016,40(02):107.[doi:10.11937/bfyy.201613028]

备注/Memo

第一作者简介：魏晓梅（1986-），女，硕士，助教，现主要从事基因组学与功能基因组学等研究工作。E-mail:15987489241@163.com.责任作者：吴丽芳（1980-），女，硕士，副教授，现主要从事植物资源的评价与利用等研究工作。E-mail:wulifang0871@163.com.基金项目：曲靖师范学院教育教学改革资助项目（JGXM2016013，JGXM201812）；校企(曲靖博浩生物科技股份有限公司)合作资助项目；云南省教育厅资助项目（2020J0632）。收稿日期：2020-01-02

更新日期/Last Update: 2021-05-10