LIN Dadu,ZHANG Yong,ZHAI Ming,et al.Preliminary Chemical Analysis AndAntioxidant Activity of the Ethanol Extract From Flower of Grevillea robusta[J].Northern Horticulture,2020,44(19):113-117.[doi:10.11937/bfyy.20194900]
银桦花醇提物成分预试及其抗氧化活性
- Title:
- Preliminary Chemical Analysis AndAntioxidant Activity of the Ethanol Extract From Flower of Grevillea robusta
- Keywords:
- the flower of Grevillea robusta; the ethanol extract; preliminary chemical analysis; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以银桦花为试材，采用化学反应鉴别法分析银桦花乙醇提取物的成分类型，并通过水杨酸法、铁氰化钾还原法、DPPH法和ABTS法评价银桦花不同极性部位的体外抗氧化活性，以期提高银桦花更为广泛的应用价值。结果表明：银桦花乙醇提取物中可能含有黄酮类、酚类、蒽醌类、强心苷和香豆素内酯类成分；不同极性部位均具有一定的抗氧化活性，且呈一定量效关系，其中水杨酸法、铁氰化钾还原法和ABTS法中乙酸乙酯部位表现出最强的抗氧化活性，而DPPH法中石油醚部位对DPPH自由基的清除能力最强。
- Abstract:
- Chemical reaction was applied to analyze the component type of theethanol extract from the flower of Grevillea robusta. Meanwhile,the antioxidant activity of different polarity partsofits flower were estimated by methods of salicylic acid,potassium ferricyanide reduction,2-diphenyl-1-picrylhydrozyl (DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS).The results showed that theethanol extract contained chemical constituents such as flavonoid,phenols,anthraquinones,cardiac glycoside and coumarins.The different polarity parts in extracts with ethanol from the flower of Grevillea robusta all had antioxidant activity.The ethyl acetate extract showed the strongest antioxidant activity in the tests of salicylic acid method,potassium ferricyanide reduction method and ABTS method.The petroleum etherextract showed the strongest scavenging activity in DPPH method than other extracts.
参考文献/References:
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备注/Memo
第一作者简介：林大都（1988-），男，硕士，讲师，现主要从事中药化学及中药资源调查等研究工作。E-mail：2016387980@qq.com.责任作者：张声源（1983-），男，博士，副教授，现主要从事中药及天然药物活性成分等研究工作。E-mail：mcdullzhang@yeah.net.基金项目：国家自然科学基金青年基金资助项目（81703662）；广东省中医药局科研资助项目（20161251）；广东省科技计划资助项目（2014A020221061，2015A090905019）；嘉应学院自然科学研究项目一般资助项目（2015KJY11，2019KJY09）。收稿日期：2019-12-27