HE Huang,YANG Yan,NIU Yu,et al.Construction Strategy of Large Fruit Tomato Core Germplasm Based on Genotype Value[J].Northern Horticulture,2020,44(15):8-14.[doi:10.11937/bfyy.20194717]
基于基因型值大果番茄核心种质的构建策略
- Title:
- Construction Strategy of Large Fruit Tomato Core Germplasm Based on Genotype Value
- Keywords:
- large fruit tomato; genotype value; genetic distance; sampling method; clustering method; core germplasm
- 文献标志码:
- A
- 摘要:
- 以多年来收集的185份大果番茄资源为研究材料,7个性状的基因型值采用混合线性模型分析方法进行预测,基于基因型预测值,分别采用2种遗传距离法、8种不同的聚类方法、3种不同的抽样方法,按照30%的抽样率构建大果番茄核心种质库。核心种质库的优劣通过方差、均值、变异系数和极差4个指标进行评价。以期构建核心种质,可为种质资源的评价和创新利用提供便利条件。结果表明:欧氏距离法差于马氏距离法,与其余7种聚类方法相比,最短距离法能够显著提高性状的变异系数和方差。采用偏离度抽样法构建的大果番茄核心种质的遗传变异大于随机抽样和优先抽样法。基于最短距离法、马氏遗传距离和偏离度抽样按照30%的抽样率获取的55份大果番茄核心资源能够充分代表原群体的遗传多样性。该研究为大果番茄资源的收集、保存与创新利用奠定了基础。
- Abstract:
- 185 large fruit tomato germplasm were taken as materials,the genotype values of 7 traits were predicted unbiased by mixed linear model analysis method.Based on genotype values,2 genetic distances,8 clustering methods,three sampling methods and 30% sampling proportion were used to construct the core germplasms of large fruit tomato.The core germplasms were evaluated based on mean,variance,range and variation coefficient.In order to construct core germplasm and facilitate the evaluation and innovation utilization of germplasm resources.The results showed that Mahalanobis distance was superior to Euclidean distance.The core germplasm constructed by the Single linkage could significantly improve the variance and variation coefficient of traits that was superior to the other 7 clustering methods.The core germplasm of large fruit tomato constructed by Deviation sampling had relatively large genetic variation,which was superior to Preferred sampling and Random sampling.The 55 core germplasm of large fruit tomato selected based on Mahalanobis distance,Single linkage,Deviation sampling and 30% sampling proportion could fully represent the genetic diversity of the initial population.This study laid a foundation for the collection,preservation and innovation utilization of large fruit tomato germplasm.
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备注/Memo
第一作者简介:贺滉(1981-),男,硕士,助理研究员,现主要从事蔬菜遗传育种等研究工作。E-mail:catasvegetable@163.com.责任作者:刘子记(1982-),男,博士,副研究员,现主要从事瓜菜遗传育种等研究工作。E-mail:liuziji1982@163.com.基金项目:海南省科技资助项目(ZDYF2018035);国家自然科学基金资助项目(31601758)。收稿日期:2019-12-13