[1]于双,邹洪涛,张玉玲,等.AM真菌、蚓粪及降解菌对土壤强还原灭菌法改良的作用机制[J].北方园艺,2020,44(21):63-70.[doi:10.11937/bfyy.20194489]
　YU Shuang,ZOU Hongtao,ZHANG Yuling,et al.Mechanism of Adding AM Fungi,Vermicompost and Degradation Bacteria to the Improvement of Reductive Soil Disinfestation[J].Northern Horticulture,2020,44(21):63-70.[doi:10.11937/bfyy.20194489]
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AM真菌、蚓粪及降解菌对土壤强还原灭菌法改良的作用机制

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年21 页码: 63-70 栏目: 出版日期: 2021-11-15
Title:
Mechanism of Adding AM Fungi,Vermicompost and Degradation Bacteria to the Improvement of Reductive Soil Disinfestation
作者:
于双; 邹洪涛; 张玉玲; 杜志德; 叶旭红
（沈阳农业大学 土地与环境学院，农业农村部东北耕地保育重点实验室，土壤肥料资源高效利用国家工程实验室，辽宁 沈阳 110866）
Author(s):
YU Shuang; ZOU Hongtao; ZHANG Yuling; DU Zhide; YE Xuhong
(College of Land and Environment，Shenyang Agricultural University/Northeast Key Laboratory of Conservation and Improvement of Cultivated Land，Ministry of Agriculture and Rural Affairs/National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources,Shenyang，Liaoning 110866)
关键词:
土壤强还原灭菌法; 番茄枯萎病; AMF; 降解菌; 蚓粪
Keywords:
reductive soil disinfestation;  Fusarium oxysporum f.sp.cucumerinun; Arbuscular Mycorrhiza fungi; degrading bacteria; vermicompost
DOI:
10.11937/bfyy.20194489
文献标志码:
A
摘要:
通过分别配施丛枝菌根真菌、降解菌、蚓粪等有机物料改良强还原土壤灭菌法，研究不同改良法对尖孢镰刀菌生长抑制作用的机制，以期找到更加有效的防治方法，解决土壤强还原灭菌法(reductive soil disinfestation，RSD)尚存在的不能完全去除土传病原菌尖孢镰刀菌(Fusarium oxysporum f.sp.cucumerinun,FOC)等问题。结果表明：添加降解菌和蚓粪使土壤pH最终达到6.0；添加蚓粪显著降低了土壤中NO-3、SO2-4含量、提高了NH+4含量；添加蚓粪和降解菌可显著提高淹水期间的土壤可培养细菌、真菌的含量，降低尖孢镰刀菌含量，落干后玉米秸秆分别添加蚓粪和降解菌的处理，其FOC含量较对照降低99.0%，且玉米秸秆配施降解菌的处理在落干期间仍持续杀灭97.0%的病原菌；玉米秸秆配施蚓粪的处理显著提高了番茄枯萎病的防治效果，防效为86.7%。说明在以玉米为有机物的RSD法中，添加蚓粪和降解菌比单独使用玉米秸秆的灭菌率更高，相同时间内抑制效果更好，能有效提高番茄枯萎病的防治效果，对番茄枯萎病的治理有重要意义。
Abstract:
The mechanism of inhibiting Fusarium oxysporum growth was studied by applying Arbuscular Mycorrhiza Fungi,degrading bacteria and vermicompost to improve the Reductive Soil Disinfestation method.In order to solve the problems of incomplete removal of Fusarium oxysporum f.sp.cucumerinun by reductive soil disinfestation in soil.The results showed that the soil pH reached 6.0 by adding degrading bacteria and vermicompost.The contents of NO-3,SO2-4 significantly reduced by adding vermicompost and increased the content of NH+4 in the soil；the contents of culturable bacteria and fungi in soil during flooding were significantly increased by adding vermicompost and degrading bacteria,and the contents of Fusarium oxysporum were reduced by 99.0% in the treatment of adding vermicompost and degrading bacteria after drying compared with the control.The treatment of degrading bacteria combined with corn straw continued to kill 97.0% of pathogenic bacteria during the drying period,and the treatment of corn straw combined with vermicompost significantly improved the control effect of Fusarium oxysporum f.sp.cucumerinun,the control effect was 86.7%.In RSD method with corn straw as organic matter,the bactericidal rate of adding vermicompost and degrading bacteria was higher than that of corn straw alone.The inhibitory effect was better in the same time,and effectively improve the control effect of Fusarium oxysporum f.sp.cucumerinun,which was of great significance to the control of Fusarium oxysporum f.sp.cucumerinun.

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备注/Memo

第一作者简介：于双（1994-），女，硕士研究生，研究方向为尖孢镰刀菌治理。E-mail：yuuushuang@126.com.责任作者：叶旭红（1980-），女，博士，副教授，硕士生导师，现主要从事尖孢镰刀菌黄瓜转化型NPS6和CPS1基因的克隆与致病机理等研究工作。E-mail：yexuhong1980@163.com.基金项目：国家自然科学基金资助项目(41301255)；辽宁省农业领域青年科技创新人才资助项目；沈阳农业大学人才引进资助项目。收稿日期：2019-11-28

更新日期/Last Update: 2021-01-21