[1]原晓龙,李子光,陆刚,等.滇牡丹查尔酮异构酶基因的克隆及表达分析[J].北方园艺,2020,44(22):79-85.[doi:10.11937/bfyy.20194324]
　YUAN Xiaolong,LI Ziguang,LU Gang,et al.Cloning and Expression Analysis of CHI of Paeonia delavayi[J].Northern Horticulture,2020,44(22):79-85.[doi:10.11937/bfyy.20194324]

点击复制

滇牡丹查尔酮异构酶基因的克隆及表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷期数: 2020年22 页码: 79-85 栏目: 出版日期: 2020-11-30

Title:: Cloning and Expression Analysis of CHI of Paeonia delavayi

作者:: 原晓龙1; 李子光2; 陆刚2; 李云琴1; 王毅1; （1.云南省林业科学院,云南省森林植物培育与开发利用重点实验室，国家林业和草原局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明 650204；2.昆明市海口林场，云南昆明 650114）

Author(s):: YUAN Xiaolong1; LI Ziguang2; LU Gang2; LI Yunqin1; WANG Yi1; (1.Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants/Conservation of Rare,Endangered ＆ Endemic Forest Plants,Public Key Laboratory of the National Forestry and Grassland Administration,Yunnan Academy of Forestry and Grassland,Kunming,Yunnan 650204；2.Kunming Haikou Forestfarm，Kunming,Yunnan 650114)

关键词:: 滇牡丹; 查尔酮异构酶; 克隆; 基因表达

Keywords:: Paeonia delavayi; chalcone isomerase; cloning; gene expression

DOI:: 10.11937/bfyy.20194324

文献标志码:: A

摘要:: 以黄花滇牡丹为试材，克隆得到2条查尔酮异构酶基因([STBX]PdCHI1和PdCHI2[STBZ])的cDNA全长，以生物信息学手段预测其功能，并检测这2条基因在花不同发育时期的表达量，以期了解滇牡丹花色的形成原因。结果表明：[STBX]PdCHI1[STBZ]基因长654 bp，编码217个氨基酸；[STBX]PdCHI2[STBZ]基因长666 bp，编码221个氨基酸；PdCHI1与圆叶牵牛(Ipomoea purpurea，AAB86474)、脐橙(Citrus sinensis，BAA36552)、大豆(Glycine max，AAT94360)和百脉根(Lotus japonicus,BAC53984)等高等植物的Ⅰ型CHI蛋白聚为一支；PdCHI2与玉米(Zea mays,NP_001151452)、大豆(Glycine max AAT94362)和葡萄(Vitis vinifera,XP_002280158)的Ⅳ型CHI蛋白聚为一支。[STBX]PdCHI1[STBZ]基因的表达量花蕾期最高，初花期和盛花期急剧降低，末花期的表达微量提升；而[STBX]PdCHI2[STBZ]基因的表达量随着花的不断发育呈现逐渐降低的趋势。

Abstract:: Two full length cDNA of the genes([STBX]PdCHI1 and PdCHI2[STBZ]) encoding chalcone isomerase were identified,carried out bioinformatics analysis and detected gene expression in the different development periods of P.delavayi.To comprehend the cause of color formation of Paeonia delavayi.The results showed that [STBX]PdCHI1[STBZ] gene had 654 bp,encodes 217 amino acids;[STBX]PdCHI2[STBZ] gene has 666 bp,encodes 221 amino acids;the phylogenetic analysis revealed PdCHI1 with Ipomoea purpurea(AAB86474),Citrus sinensis(BAA36552),Glycine max(AAT94360) and Lotus japonicas(BAC53984) clustered one clan belonging to type Ⅰ CHI from higher plants;PdCHI2 with Zea mays(NP_001151452),Glycine max(AAT94362) and Vitis vinifera(XP_002280158) clustered the same clan appertaining type Ⅳ CHI.In the different developmental periods of P.delavayi flower,the expression level of [STBX]PdCHI1[STBZ] gene was the highest at budding stage,reduced sharply at initial flowering stage and full-bloom stage,elevated slightly at blossom falling stage;however,when the flower was developing,the [STBX]PdCHI2[STBZ] gene expression level presented the gradually lower trend.

参考文献/References:

[1]TREUTTER D.Significance of flavonoids in plant resistance:A review[J].Environmental Chemistry Letters,2006,4(3):147-157.[2]RALSTON L,SUBRAMANIAN S,MATSUNO M,et al.Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II chalcone isomerases[J].Plant Physiology,2005,137(4):1375-1388.[3]CHENG A X,ZHANG X,HAN X J,et al.Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids[J].New Phytologist,2018,217(2):909-924.[4]SHIMADA N,AOKI T,SATO S,et al.A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus[J].Plant Physiology,2003,131(3):941-951.[5]LIUY,ZHAO S Z,WANG J S,et al.Molecular cloning,expression,and evolution analysis of type II CHI gene from peanut (Arachis hypogaea L.)[J].Development Genes & Evolution,2015,225(1):1-10.[6]NGAKI M N,LOUIE G V,PHILIPPE R N,et al.Evolution of the chalcone isomerase fold from fatty acid-binding to stereospecific enzyme[J].Nature,2012,485(7399):530-533.[7]GENSHEIMER M,MUSHEGIAN A.Chalcone isomerase family and fold:No longer unique to plants[J].Protein Science,2010,13(2):540-544.[8]MORITA Y,TAKAGI K,FUKUCHI-MIZUTANI M,et al.A chalcone isomerase-like protein enhances flavonoid production and flower pigmentation[J].Plant Journal,2014,78(2):294-304.[9]HONG D Y,PAN K Y,YU H.Taxonomy of the Paeonia delavayi complex (Paeoniaceae)[J].Annals of the Missouri Botanical Garden,1998,85(4):554-564.[10]龚洵,潘跃芝,杨志云.滇牡丹的多样性和现状评估[J].西北植物学报,2003,23(2):218-223.[11]HUA M,MA H F,TAN R,et al.Determination of anthocyanins and flavonols in Paeonia delavayi by high-performance liquid chromatography with diode array and mass spectrometric detection[J].Analytiacal Letters,2018,51:2331-2339.[12]周琳,王雁,律春燕,等.云南野生黄牡丹花色素成分的鉴定[J].东北林业大学学报,2011,39(8):52-54.[13]FIGUEIREDO D D,BATISTA R A,ROSZAK P J,et al.Auxin production in the endosperm drives seed coat development in Arabidopsis[J].Elife,2016(5):e20542.[14]贺窑青,马履一,桑子阳.红花玉兰花色形成的初步研究[J].西北植物学报,2010,30(11):2252-2257.[15]MUIR S R,COLLINS G J,ROBINSON S,et al.Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols[J].Nature Biotechnology,2001,19(5):470-474.[16]WENG J K,CHAPPLE C.The origin and evolution of lignin biosynthesis[J].New Phytologist,2010,187(2):273-285.[17]CHEN T,ZHANG X,JIANG W,et al.Preparation of plant derived carbon and its application for inclusion pigments[J].Advanced Powder Technology,2018,29(12):3040-3048.[18]LUCERI C,GIOVANNELLI L,PITOZZI V,et al.Liver and colon DNA oxidative damage and gene expression profiles of rats fed Arabidopsis thaliana mutant seeds containing contrasted flavonoids[J].Food & Chemical Toxicology,2008,46(4):1213-1220.[19]BUTELLI E,TITTA L,GIORGIO M,et al.Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors[J].Nature Biotechnology,2008,26(11):1301-1308.[20]GROTEWOLD E.The genetics and biochemistry of floral pigments[J].Annual Review of Plant Biology,2006,57(1):761-780.[21]DASTMALCHI M,DHAUBHADEL S.Soybean chalcone isomerase:Evolution of the fold,and the differential expression and localization of the gene family[J].Planta,2015,241(2):507-523.[22]王蕊,李彦慧,郑健.紫丁香查尔酮异构酶基因SoCHI的克隆及表达分析[J].植物资源与环境学报,2018,27(3):11-17.

相似文献/References:

[1]雷　桅,邹　祥,向　阳,等.植物查尔酮异构酶的生物信息学分析[J].北方园艺,2008,32(02):0.[doi:10.11937/bfyy.200802081]
　LEI Wei,ZOU Xiang,XIANG Yang,et al.A Bioinformatic Analysis on Chalcone Isomerase in Plant[J].Northern Horticulture,2008,32(22):0.[doi:10.11937/bfyy.200802081]
[2]原晓龙,陈剑,陈中华,等.滇牡丹类黄酮5-O-葡萄糖基转移酶基因的鉴定及表达分析[J].北方园艺,2018,42(14):102.[doi:10.11937/bfyy.20174499]
　YUAN Xiaolong,CHEN Jian,CHEN Zhonghua,et al.Identification and Expression Analysis of a Flavonoid 5-O-glucosyltransferase Gene in Paeonia delavayi[J].Northern Horticulture,2018,42(22):102.[doi:10.11937/bfyy.20174499]
[3]刘子榕,平怀磊,蒲艳,等.植物生长调节剂对不同基质滇牡丹嫩硬枝扦插的效果[J].北方园艺,2022,(23):74.[doi:10.11937/bfyy.20221491]
　LIU Zirong,PING Huailei,PU Yan,et al.Effects of IBA on the Cuttage Propagation of Young Hard Shoots of Paeonia delavayi Under Different Substrates[J].Northern Horticulture,2022,(22):74.[doi:10.11937/bfyy.20221491]
[4]杜春,平怀磊,王文治,等.滇牡丹PdANR基因克隆及表达模式分析[J].北方园艺,2022,(24):42.[doi:10.11937/bfyy.20221618]
　DU Chun,PING Huailei,WANG Wenzhi,et al.Cloning and Expression Pattern Analysis of PdANR Gene From Paeonia delavayi[J].Northern Horticulture,2022,(22):42.[doi:10.11937/bfyy.20221618]

备注/Memo

第一作者简介：原晓龙(1986-)，男，硕士，助理研究员，现主要从事林木分子生物学等研究工作。E-mail：xiaolony@126.com.责任作者：王毅(1981-)，男，博士，副研究员，现主要从事分子生物学和植物学等研究工作。E-mail：22825818@qq.com.基金项目：国家自然科学基金资助项目(31860177)；云南省应用基础研究青年资助项目(2016FD100);云南省林业科学院创新基金资助项目(QN2018-01)。收稿日期：2019-11-21

更新日期/Last Update: 2021-02-06