TIAN Songqing,YUAN Ruyou,WEI Qinhua,et al.Tissue Culture and Root Tuber Induction of Dahlia pinnate‘Filogo’[J].Northern Horticulture,2020,44(07):79-85.[doi:10.11937/bfyy.20192453]
大丽花‘费罗加’组织培养和块根诱导
- Title:
- Tissue Culture and Root Tuber Induction of Dahlia pinnate‘Filogo’
- Keywords:
- Dahlia pinnate ‘Filogo’; stem segment; primary culture; proliferation culture; adventitious root and tuber induction
- 文献标志码:
- A
- 摘要:
- 以大丽花品种‘费罗加’为试材,茎段作为外植体,探讨了不同基本培养基、激素组合、蔗糖浓度、光照时间等对诱导愈伤组织、不定芽、不定根和块根的影响,以期缩短块根生产周期。结果表明:初代培养在MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+蔗糖30 g·L-1+琼脂6 g·L-1上愈伤组织诱导率为54.9%,褐化率为44.8%,污染率为26.6%,成活率达69.6%。继代增殖培养最佳培养基为MS+6-BA 2.0 mg·L-1+NAA 0.01 mg·L-1+蔗糖30 g·L-1+琼脂6 g·L-1,增殖系数为7.8,愈伤组织诱导率22.9%。生根最佳培养基为WPM+NAA 1.0 mg·L-1+6-BA 0.1 mg·L-1+蔗糖60 g·L-1+琼脂6 g·L-1+炭粉2 g·L-1,最适宜光照时间为6 h,生根率为98.5%,根系密集健壮。块根诱导最适材料为带根瓶苗,出现初步形态的块根,形成块根率达56.8%。
- Abstract:
- The effects of different basic medium,hormone combinations,sucrose concentration and light duration on callus induction,adventitious bud induction,adventitious root induction and root tuber induction were studied by using Dahlia pinnate ‘Filogo’ stem segments as explants,in order shorten the production cycle of root tuber.The results showed that the callus induction rate,browning rate,contamination rate and survival rate were 54.9%,44.8%,26.6% and 69.6% respectively on MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+sucrose 30 g·L-1+agar 6 g·L-1.The optimum medium for subculture was MS+6-BA 2.0 mg·L-1+NAA 0.01 mg·L-1+sucrose 30 g·L-1+agar 6 g·L-1,the proliferation coefficient was 7.8,and the callus induction rate was 22.9%.The optimum medium for rooting was WPM+NAA 1.0 mg·L-1+6-BA 0.1 mg·L-1+sucrose 60 g·L-1+agar 6 g·L-1+carbon powder 2 g·L-1.The optimum illumination time was 6 hours per day,the rooting rate was 98.5%,and the roots were dense and robust.The optimum material for root induction was bottle seedling with roots.The rate of root tuber formation with initial morphology was 56.8%.
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备注/Memo
第一作者简介:田松青(1974-),女,硕士,副教授,高级工程师,现主要从事观赏植物资源利用与繁育等研究工作。E-mail:tiansongqing@126.com.责任作者:朱旭东(1972-),男,博士,教授,现主要从事花卉育种和产业化等研究工作。E-mail:13862130525@163.com.基金项目:江苏省 ‘333工程’中青年学术带头人资助项目(BRA2018375);江苏现代农业(花卉)产业技术体系建设资助项目(JATS[2019]365);苏州农业职业技术学院科研创新团队建设资助项目(CXTD201801)。收稿日期:2019-09-10