ZHAO Huijun,LIANG Xinxin,WEI Yuqing.Effect of Different Concentrations of Cu,Zn and Mn on the Photosynthetic and Antioxidative System of Phytolaccca americana[J].Northern Horticulture,2020,44(03):120-127.[doi:10.11937/bfyy.20191911]
不同浓度的Cu、Mn、Zn胁迫对商陆叶片光合系统参数及抗氧化酶系统的影响
- Title:
- Effect of Different Concentrations of Cu,Zn and Mn on the Photosynthetic and Antioxidative System of Phytolaccca americana
- Keywords:
- Phytolacca americana; heavy metal treatment; photosynthetic parameters; antioxidative system
- 文献标志码:
- A
- 摘要:
- 以重金属超富集植物商陆为试材，采用水培处理方式，分别设置不同浓度梯度的Cu、Zn和Mn处理，通过测定不同处理下第5、8、12、24天时商陆叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性，以及第12、24天时对成熟叶片的光合系统参数（光合速率、气孔导度、胞间CO2浓度、蒸腾速率）等指标，以期揭示商陆对不同重金属的响应机理，为重金属污染土壤的植物修复提供参考依据。结果表明：Cu和Zn处理后虽然提高了SOD和POD活性，但是极显著的抑制了CAT活性；Cu和Zn显著抑制商陆的光合速率和气孔导度，导致细胞间CO2浓度极显著增加，蒸腾速率降低；Cu和Zn处理导致叶片叶绿素含量极显著下降、出现缺绿和坏死斑点等毒害症状，显著降低了植株的生物产量和相对生长速率，但是Mn胁迫对商陆光合参数及叶绿素含量的影响较轻，商陆在叶片中保持着稳定的CAT活性，这可能是商陆耐受Mn毒害的重要机制。
- Abstract:
- The heavy metal hyperaccumulator Phytolacca americana was used as material,the effect of different concentrations of Cu,Zn and Mn on SOD,POD and CAT activities after 5,8,12,24 days of stress under hydroponic conditions were studied,and the effect on the photosynthetic system parameters (photosynthetic rate,stomatal conductance,intercellular CO2 concentration,transpiration rate) were also studied,the purpose was to reveal the response mechanism of Phytolacca americana to different heavy metals and provide theoretical basis for phytoremediation of heavy metal contaminated soil.The results showed that although the activities of SOD and POD were increased after Cu and Zn treatment,the significant inhibition of CAT activities disturb the antioxidant enzyme system.Cu and Zn significantly inhibited the photosynthetic rate and stomatal conductance of Phytolacca americana,resulting in an extremely increase in intercellular CO2 concentration and a decrease in transpiration rate.Toxic symptoms such as chlorosis and necrotic spots appeared under Cu and Zn treatment;Cu and Zn could significantly reduce plant biomass and relative growth rate,but after Mn treatment,biological yield did not decline,and stable antioxidant enzyme activity was maintained in leaves,which might be an important mechanism of Mn toleration in Phytolacca americana.
参考文献/References:
[1]WAN D,SONG L,MAO X,et al.One-century sediment records of heavy metal pollution on the southeast Mongolian plateau:Implications for air pollution trend in China[J].Chemosphere,2019,220:539-545.[2]QUINA A S,DURAO A F,MUNOZ et al.Population effects of heavy metal pollution in wild Algerian mice (Mus spretus)[J].Ecotoxicology and Environmental Safety,2019,171:414-424.[3]FENG Y,CHENG L L,BOWEN W.Evaluation of heavy metal pollution in the sediment of Poyang Lake based on stochastic geo-accumulaion model (SGM)[J].Science of The Total Environment,2019,659:1-6.[4]MAO C,SONG Y,CHEN L,et al.Human health risks of heavy metals in paddy rice based on transfer characteristics of heavy metals from soil to rice[J].Catena,2019,175:339-348.[5]ZHONG D X,ZHONG Z P,WU L H,et al.Thermal characteristics and fate of heavy metals during thermal treatment of Sedum plumbizincicola,a zinc and cadmium hyperaccumulator[J].Fuel Processing Technology,2015,131:125-132.[6]ZHANG X,LIN L,CHEN M,et al.A nonpathogenic Fusarium oxysporum strain enhances phytoextraction of heavy metals by the hyperaccumulator Sedum alfredii Hance[J].Journal of Hazardous Materials,2012,229-230:361-370.[7]SINGH R,JHA A B,MISRA A N,et al.Chapter 13-Adaption mechanisms in plants under heavy metal stress conditions during phytoremediation[M].Phytomanagement of Polluted Sites:Elsevier,2019:329-360.[8]HU Y,TIAN S,FOYER C H,et al.Efficient phloem transport significantly remobilizes cadmium from old to young organs in a hyperaccumulator Sedum alfredii[J].Journal of Hazardous Materials,2019,365:421-429.[9]陈焱山,贾梦茹,曹越,等.蜈蚣草砷富集的分子机制研究进展[J].农业环境科学学报,2018,37(7):1402-1408.[10]YUAN M,TIE B Q,TANG M Z,et al.Accumulation and uptake of manganese in a hyperaccumulator Phytolacca americana[J].Minerals Engineering,2007,20(2):188-190.[11]RALPH P J,BURCHETT M D.Photosynthetic response of Halophila ovalis to heavy metal stress[J].Environmental Pollution,1998,103(1)：91-101.[12]MENG Y,ZHANG L,WANG L Q,et al.Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress[J].Ecotoxicology and Environmental Safety,2019,173:214-224.[13]代其林.水杨酸对低温下水稻幼苗生理生化特性的影响[D].成都：四川大学，2004.[14]吴倩.CAT调控水稻叶片光呼吸过程中抗氧化系统的机理研究[D].扬州：扬州大学，2013.[15]王学征,韩文灏,于广建．盐分胁迫对番茄幼苗生理生化指标影响的研究[J].北方园艺，2004(3):48-49.[16]胡家恕,邵爱萍,任爱霞.SOD活性和同工酶检测系统中干扰因素的研究[J].浙江大学学报（农业与生命科学版），2004，28(2):179-182.[17]孙云．茶叶抗坏血酸过氧化物酶的生理学与分子生物学研究[D].福州：福建农林大学，2009.[18]HASHEMI S A.Studying the effects of heavy metal on chlorophyll and sugar in one-year-old seedlings organs of Acer velutinum specie[J].Acta Ecologica Sinica,2018,38(3):224-227.[19]CHENG S,TAM NFY,LI R,et al.Temporal variations in physiological responses of Kandelia obovata seedlings exposed to multiple heavy metals[J].Marine Pollution Bulletin,2017,124(2):1089-1095.[20]KHANNA K,JAMWAL V L,KOHLI S K,et al.Plant growth promoting rhizobacteria induced Cd tolerance in Lycopersicon esculentum through altered antioxidative defense expression[J].Chemosphere,2019,217:463-474.[21]ZHAO H J,WU L Q,CHAI T Y,et al.The effects of copper,manganese and zinc on plant growth and elemental accumulation in the manganese-hyperaccumulator Phytolacca americana[J].Journal of Plant Physiology,2012,169:1243-1252.[22]张玉秀,黄智博,张红梅,等.商陆和烟草对锰胁迫的抗氧化响应研究[J].环境科学，2009,30(12)：3676-3683.[23]祖元刚,王文杰,陈华峰,等.豚草叶片和果实气体交换特性与11种土壤重金属相关性[J].应用生态学报，2006(12):2321-2326.[24]胡彦,陈沁,邓志瑞,等.辣椒光合系统对重金属Cd(2+)胁迫的响应[J].上海大学学报(自然科学版)，2005(5):531-534.[25]明华,曹莹,胡春胜,等.铅胁迫对玉米光合特性及产量的影响[J].玉米科学,2008(1):74-78.[26]CHENG S,TAM NFY,LI R,et al.Temporal variations in physiological responses of Kandelia obovata seedlings exposed to multiple heavy metals[J].Marine Pollution Bulletin,2017,124(2):1089-1095.[27]CHEN S,WANG Q,LU H,et al.Phenolic metabolism and related heavy metal tolerance mechanism in Kandelia Obovata under Cd and Zn stress[J].Ecotoxicology and Environmental Safety,2019,169:134-143.[28]JIANG K,WU B,WANG C,et al.Ecotoxicological effects of metals with different concentrations and types on the morphological and physiological performance of wheat[J].Ecotoxicology and Environmental Safety,2019,167:345-353.[29]楚晶晶.铅锌复合胁迫对大叶女贞叶片光系统性能的影响[D].长沙:中南林业科技大学,2019.[30]申须仁,董名扬,王朝勇,等.高锰胁迫对香根草矿质元素吸收及光合系统的影响[J].农业环境科学学报,2019,38(10):2297-2305.[31]ZHAO H J,WEI Y Q,WANG J,et al.Isolation and expression analysis of cadmium-induced genes from Cd/Mn hyperaccumulator Phytolacca americana[J].Plant Biology,2019,21:15-24.[32]朱金文.高锰胁迫下空心莲子草的生理生化特性和草甘膦耐性研究[D].杭州:浙江大学,2008.
相似文献/References:
[1]梁娟,危革.不同处理对商陆种子发芽率的影响[J].北方园艺,2012,36(10):184.
LIANG Juan,WEI Ge.Effects of Different Pretreatment on the Germination Percentage of Pokeberry Seed[J].Northern Horticulture,2012,36(03):184.
[2]张丽珍,杨冬业,刘杰,等.超富集植物商陆多倍体诱导研究[J].北方园艺,2014,38(16):109.
ZHANG Li-zhen,YANG Dong-ye,LIU Jie,et al.Study on Polyploid Induction of Hyperaccumulator Plant—Phytolacca acinosa Roxb.[J].Northern Horticulture,2014,38(03):109.
备注/Memo
第一作者简介：赵会君(1980-)，女，博士，讲师，研究方向为植物抗逆生物学。E-mail:zhaohuijun1022@163.com.责任作者：魏玉清（1969-），男，博士，教授，研究方向为植物抗逆生物学。E-mail:weiyuqing@126.com.基金项目：北方民族大学校级重点科研资助项目（2017KJ31）。收稿日期：2019-08-06