ZHANG Qinghua,GUO Cong,XIANG Fayun,et al.Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques[J].Northern Horticulture,2020,44(01):7-12.[doi:10.11937/bfyy.20191871]
利用EMS和炭疽病粗毒素诱变筛选草莓抗炭疽病突变体
- Title:
- Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques
- 文献标志码:
- A
- 摘要:
- 以甲基磺酸乙酯（EMS）和暹罗炭疽菌（C.siamense）粗毒素为诱变剂，‘晶瑶’组培苗叶柄为原始材料，建立了抗炭疽病草莓材料诱变筛选体系，并筛选出了抗草莓炭疽病的突变体。结果表明：以0.8%的EMS溶液处理2 h可获得半致死效应；炭疽菌粗提物浓度为4%时为适宜的抗病选择压。对532个不定芽进行2次不连续离体筛选后获得43个抗性芽，成活率为8.1%。抗性芽再生植株移栽存活后，经炭疽菌离体接种鉴定，初步获得了抗炭疽病的草莓‘晶瑶’突变体株系13株，为后续进一步研究草莓炭疽病抗性机制提供了材料。
- Abstract:
- The petioles of strawberry cultivar ‘Jingyao’ in vitro were treated with chemical mutagen ethyl methane sulfonate（EMS）and the crude toxin produced by Colletotrichum siamense Nj-2，the pathogenic strain of straberry anthracnose.As a result，the system of in vitro screening some resistant mutants for strawberry anthracnose was established and the resistant mutants were obtained.The results showed that the petioles were treated with 0.8% of EMS for 2 hours which resulted in the effect of the ‘semi lethal dose’,and the appropriate selection pressure was that the petioles were cultured in medium supplemented with 4% of Nj-2 crude extracts.There were 43 regenerated buds survival in 532 mutation and after twice discontinuous screening the survival rate was 8.1%,and of which 12 strains were identified as resistant mutants after inoculated with C.siamense isolate Nj-2 on leveas and petioles of mutans in vitro.This study provided materials for further study on the mechanism of strawberry anthracnose resistance.
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备注/Memo
第一作者简介：张庆华（1979-），女，博士，副研究员，现主要从事逆境生理及分子育种等研究工作。E-mail：zzqqhh624@163.com.责任作者：韩永超（1982-），男，博士，副研究员，现主要从事草莓资源评价及抗性育种等研究工作。E-mail：hyc660@126.com.基金项目：湖北省技术创新专项-重大资助项目（2018ABA071）；湖北省农业科技创新中心资助项目(2016-620-000-001-014)。收稿日期：2019-07-31