HUANG Lijuan,TANG Huajiang,ZHAO Lili,et al.Analysis of Genetic Diversity of Paspalum dilatatum Germplasms Based on Phenotypic Traits[J].Northern Horticulture,2020,44(08):60-66.[doi:10.11937/bfyy.20191787]
基于表型性状的毛花雀稗遗传多样性分析
- Title:
- Analysis of Genetic Diversity of Paspalum dilatatum Germplasms Based on Phenotypic Traits
- Keywords:
- Paspalum dilatatum; genetic diversity; phenotypic trait; principal component analysis; cluster analysis
- 文献标志码:
- A
- 摘要:
- 以6份不同来源的毛花雀稗种质为试材，测定其11个表型性状，计算各表型性状的变异系数和Shannon-Weaver指数，并进行主成分分析和聚类分析，以期揭示毛花雀稗种质间的遗传差异。结果表明：11个表型性状变异系数平均值为38.65%，其茎节间长度、茎长粗比、鲜质量和干质量4个指标变异系数均超过50%；株高的Shannon-Weaver指数最低，生长速度的最高。株高与茎节直径和叶宽呈极显著相关，相关系数分别为0.961、0.921（P<0.01）；鲜质量与株高和茎节直径的相关系数为0.852和0.869，呈显著相关（P<0.05）；而干质量与株高呈显著相关（P<0.05），与茎节直径和鲜质量呈极显著相关（P<0.01），相关系数分别为0.861、0.925、0.971。前3个主成分的累积贡献率达95.24%，贡献较大的株高、叶长、鲜质量和干质量4个因子可以作为毛花雀稗种质的综合评价指标。聚类分析显示毛花雀稗种质群体可分为2个大类群，与其它种质相比地理距离最大的美国种质为第一类群，来自亚洲的5个种质为第二类群，而第二类群又可以进一步分为2个亚类群，越南种质单独为一个亚类群，来自贵州的4个种质为一个亚类群。可见美国种质与亚洲种质有一定的遗传差异，可在育种中挖掘。
- Abstract:
- To reveal the genetic variations of six Paspalum dilatatum germplasms derived from different regions,11 phenotypic traits.The variation coefficient and Shannon-Weaver index were calculated for each phenotypic trait,and then principal component analysis and cluster analysis were also conducted.The results showed that the average variation coefficient of 11 phenotypic traits was 38.65%,and the variation coefficient of internode length growth rate,internode length/stem diameter ratio,fresh mass and dry mass were higher than 50%.The Shannon-Weaver index was the lowest in plant height and highest in growth rate.Plant height,was hightly significant correlation with stem diameter and leaf width,the correlation coefficients were 0.961 and 0.921 (P<0.01),respectively.The fresh mass′ correlation coefficients of plant height and stem diameter were 0.852 and 0.869,which showing a significant correlation (P<0.05).However,dry mass was significant correlation with plant height (P<0.05),and hightly significant correlation with stem diameter and fresh mass (P<0.01),the correlation coefficients were 0.861,0.925,0.971,respectively.The cumulative contribution rate of the first three principal components was 95.24%,plant height,leaf length,fresh mass and dry mass were main contributors of these three principal components,and they could be identified as an proxy for comprehensive evaluation.The cluster analysis showed that the six germplasms could be
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备注/Memo
第一作者简介：黄莉娟（1994-），女，贵州榕江人，硕士研究生，研究方向为牧草种质资源及育种。E-mail：huanglj615@163.com.责任作者：赵丽丽（1981-），女，内蒙通辽人，博士，教授，研究方向为牧草种质资源。E-mail：zhaolili_0508@163.com.基金项目：山地植物资源保护与种质创新教育部重点实验室开放课题资助项目（MOELP-201701）；贵州省科技计划重大专项资助项目（黔科合重大专项字[2016]3002号）；贵州省高层次创新型人才资助项目（黔财教[2017]45号）。收稿日期：2019-08-02