MAO Lianzhen,TANG Di,ZHU Haishan,et al.Changes of Defense Enzymes in Tomato Leaves of Inbred Lines With Different Resistance After TSWV Infection[J].Northern Horticulture,2020,44(03):44-4.[doi:10.11937/bfyy.20191735]
感染TSWV后不同抗性番茄栽培自交系叶片防御酶变化
- Title:
- Changes of Defense Enzymes in Tomato Leaves of Inbred Lines With Different Resistance After TSWV Infection
- Keywords:
- tomato; tomato spotted wilt virus; disease resistance mechanism; physiological indicators; defense enzymes
- 文献标志码:
- A8
- 摘要:
- 选取3个对番茄斑萎病毒（TSWV）表现不同抗性的番茄栽培自交系YNAU335（免疫）、96172I（感病）和5号（感病），利用紫外分光光度法，研究接种TSWV后相关防御酶活性的变化，揭示YNAU335对TSWV的抗病生理机制。结果表明：在对照条件下，YNAU335自交系中超氧化物歧化酶（SOD）、过氧化物酶（POD）、几丁质酶（CHT）、β-1,3-葡聚糖酶（β-1,3-GA）和多酚氧化酶（PPO）活性高于96172I和5号自交系，苯丙氨酸解氨酶（PAL）活性低于2个感病自交系；感染TSWV后，3个自交系中SOD、POD、CHT和β-1,3-GA活性均高于对照，PPO活性低于对照，YNAU335中PAL活性高于对照，2个感病自交系的PAL活性低于对照；感染TSWV后YNAU335中的6种酶的活性均高于2个感病自交系。
- Abstract:
- Three tomato inbred lines with different resistance to tomato spot wilt virus (TSWV) were selected,namely YNAU335 (immunity),96172I (susceptibility) and No.5 (susceptibility).The changes of defense enzyme activity after inoculation of TSWV were studied by UV spectrophotometry,and to reveal the physiological mechanism of YNAU335 resistance to TSWV.The results showed that the activities of SOD,POD,CHT,β-1,3-GA and PPO in YNAU335 inbred lines were all higher than those in the inbred lines 96172I and No.5,while the activities of phenylalanine ammonia-lyase were lower than those in the two inbred lines;after TSWV infection,the activities of SOD,POD,CHT and β-1,3-GA in three inbred lines were higher than those in the control,while the activities of PPO were lower than those in the control.The activities of PAL in YNAU335 were higher than those in the control,while those in two susceptible inbred lines were lower than those in the control.After TSWV infection,the activities of six enzymes in YNAU335 were higher than those in the other two susceptible inbred lines.
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备注/Memo
第一作者简介：毛莲珍（1994-），女，硕士研究生，研究方向为蔬菜遗传育种。E-mail:348400811@qq.com.责任作者：赵凯（1981-），男，博士，副教授，现主要从事蔬菜遗传育种等研究工作。E-mail:343810456@qq.com.基金项目：国家地区科学基金资助项目（31660576，31760583）;云南省科技计划面上资助项目（2016FB064）。收稿日期：2019-07-31