[1]李英楠,曹正,杜南山,等.三种PGPR菌株对黄瓜生长及根际土壤环境的影响[J].北方园艺,2019,43(24):21-27.[doi:10.11937/bfyy.20191643]
　LI Yingnan,CAO Zheng,DU Nanshan,et al.Effects of Three PGPR Strains on Cucumber Growth and Rhizosphere Soil Environment[J].Northern Horticulture,2019,43(24):21-27.[doi:10.11937/bfyy.20191643]

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三种PGPR菌株对黄瓜生长及根际土壤环境的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年24 页码: 21-27 栏目: 出版日期: 2019-12-30

Title:: Effects of Three PGPR Strains on Cucumber Growth and Rhizosphere Soil Environment

作者:: 李英楠; 曹正; 杜南山; 国志信; 朴凤植; （河南农业大学园艺学院，河南郑州 450002）

Author(s):: LI Yingnan; CAO Zheng; DU Nanshan; GUO Zhixin; PIAO Fengzhi; (College of Horticulture，Henan Agricultural University，Zhengzhou，Henan 450002)

关键词:: 植物根际促生菌; 黄瓜; 生长; 品质; 土壤环境

Keywords:: PGPR; cucumber; growth; quality; soil environment

DOI:: 10.11937/bfyy.20191643

文献标志码:: A

摘要:: 为探讨植物根际促生菌（PGPR）多粘类芽孢杆菌（Paenibacillus polymyxa）G15-7、NSY50和绿针假单胞菌（Pseudomonas chlomraphis）HG28-5在设施栽培黄瓜上的应用效果，以‘博杰605’黄瓜为试验材料，采用菌液灌根方法，研究其对黄瓜植株生长、果实品质和根际土壤环境的影响，以期改善黄瓜土壤环境促进其生长。结果表明：3种菌株能够促进黄瓜植株生长、提高叶绿素含量和根系活力、改善果实营养品质；同时提高根际土壤蔗糖酶、脲酶、磷酸酶和过氧化酶的活性，增加根际土壤细菌、放线菌的数量以及根际土壤中速效氮、磷、钾的含量，有利于改善黄瓜根际土壤环境。3种菌株中，G15-7处理效果最佳，具有一定的开发利用潜力。

Abstract:: To explore the application effect of plant growth promoting rhizobacteria(PGPR) Paenibacillus polymyxa G15-7,NSY50 and Pseudomonas chlomraphis HG28-5 on cucumber in greenhouse production,‘Bojie 605’ cucumber was used as the experimental material，and bacterial liquid root irrigation method was used to study their effects on cucumber plant growth，fruit quality and rhizosphere soil environment，so as to improve cucumber soil environment and promote its growth.The results showed that the three strains could improve the growth of cucumber plants，the chlorophyll content and root activity，and the fruit nutritional quality，the activities of sucrase，urease，phosphatase and peroxidase in rhizosphere soil，the number of bacteria and actinomycetes in rhizosphere soil and the content of available nitrogen，phosphorus and potassium in rhizosphere soil.Among the three strains，G15-7 had the best treatment effect and had certain potential for development and utilization.

参考文献/References:

[1]毛爱军,陈春秀,温常龙,等.春秋保护地黄瓜新品种京研109的选育[J].中国蔬菜,2019(2):69-72.[2]刘金秀,马正,申屠旭萍,等.黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定[J].园艺学报,2012,39(6):1123-1130.[3]李欢可.微生物有机肥对土壤改良及烟草钾素累积规律研究[D].长沙:湖南大学,2013.[4]闫洪雪,刘露,李丽,等.PGPR的研究进展及其在农业上的应用[J].黑龙江农业科学,2016(6):148-151.[5]崔文艳.B9601-Y2溶磷解钾固氮能力及防病促进玉米生长效果研究[C].北京：中国植物病理学会2018年学术年会论文集,2018.[6]DU N S,SHI L,YUAN Y H,et al.Isolation of a potential biocontrol agent Paenibacillus polymyxa NSY50 from vinegar waste compost and its induction of host defense responses against Fusarium wilt of cucumber[J].Microbiological Research,2017,202:1-10. [7]ZAMIODIS C,KORTELAND J,VANPELT J A,et al.Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB72 expression in Arabidopsis roots during onset of induced systemic resistance and iron-deficiency responses[J].The Plant Journal,2015,84(2):309-322.[8]AHMAD Y N,WAHEED A,ULLAH K W,et al.Halotolerant plant-growth promoting rhizobacteria modulate gene expression and osmolyte production to improve salinity tolerance and growth in Capsicum annum L.[J].Environmental Science and Pollution Research,2018,25(23):26236-26250.[9]于迪,吕雅悠,余本玉,等.微生物肥对辣椒生长及疫病防治效果[J].北方园艺,2015(12):162-165.[10]王娟.绿针假单胞菌HG28-5对辣椒生长及根际土壤微生态的影响[C].许昌：河南省植物保护学会第十一次、河南省昆虫学会第十次、河南省植物病理学会第五次会员代表大会暨学术讨论会论文集,2017.[11]SHI L,DU N S,SHU S,et al.Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community[J].Scientific Reports,2017,7:41234.[12]胡秉芬,黄华梨,季元祖,等.分光光度法测定叶绿素含量的提取液的适宜浓度[J].草业科学,2018,35(8):1965-1974.[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2001:119-120.[14]李嫚.生物制剂与缓释肥在日光温室黄瓜和辣椒的应用初探[D].郑州:河南农业大学,2018.[15]丁方丽,李嫚,刘东平,等.植物根际促生菌普城沙雷菌A21-4对黄瓜生长及土壤微生态的影响[J].中国蔬菜,2018(5):36-41.[16]秘洪雷.杨树人工林对滴灌施肥的生长及生理响应研究[D].北京:中国林业科学研究院,2017.[17]李立平,张佳宝,邢维芹,等.土壤速效氮磷钾测定进展[J].土壤通报,2003(5):483-488.[18]郭英,杨萍,张丹雨,等.野大豆多功能根际促生菌的筛选鉴定和促生效果研究[J].生物技术通报,2018,34(10):108-115.[19]王广印,郭卫丽,王胜楠,等.秸秆、生物菌肥和土壤调理剂施用对日光温室越冬茬番茄生长、坐果及果实品质的影响[J].中国农学通报,2018,34(15):55-59.[20]王娟,刘东平,丁方丽,等.促植物生长根际细菌HG28-5对黄瓜苗期生长及根际土壤微生态的影响[J].中国蔬菜,2016(8):50-55.[21]张典利,孟臻,亓文哲,等.植物根际促生菌的研究与应用现状[J].世界农药,2018,40(6):37-43.[22]王继元.促植物生长根际细菌对辣椒的促生作用及其机制初步研究[D].郑州:河南农业大学,2014.[23]曹丹,宗良纲,肖峻,等.生物肥对有机黄瓜生长及土壤生物学特性的影响[J].应用生态学报,2010,21(10):2587-2592.[24]周健民.土壤学大辞典[M].北京:科学出版社,2013.[25]GU J,LI S X,QIN Q J,et al.Changes of some hydrolase activities during agricultural waste materials composting in high temperature and static state[J].Chinese Agricultural Science Bulletin,2005,21(5):32-35.

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备注/Memo

第一作者简介：李英楠（1994-），女，硕士研究生，研究方向为菜田生态与环境。E-mail：820034722@qq.com.责任作者：朴凤植（1965-），男，博士，教授，硕士生导师，现主要从事设施蔬菜栽培与生理学等研究工作。E-mail：piao1203@163.com.基金项目：国家大宗蔬菜产业技术体系专项资助项目（CARS-23-B03）。收稿日期：2019-07-18

更新日期/Last Update: 2019-12-30