CHENG Huanhuan,YAO Weiwei,PENG Lijuan,et al.Screening of an Antagonistic Bacillus Strain Against Pepper Anthracnose Disease[J].Northern Horticulture,2019,43(23):6-11.[doi:10.11937/bfyy.20191145]
一株辣椒炭疽病生防芽孢杆菌的筛选
- Title:
- Screening of an Antagonistic Bacillus Strain Against Pepper Anthracnose Disease
- Keywords:
- Bacillus; biological control; pepper anthracnose
- 文献标志码:
- A
- 摘要:
- 从贵州省安龙县辣椒炭疽病发病地块采集健康辣椒根际土壤，通过平板对峙法获得具有明显拮抗辣椒炭疽病菌(Colletotrichum capsici)的1株芽孢杆菌，通过形态学和生理生化特征观察,并结合16S rDNA、gyrA基因序列分析结果构建系统发育树。结果表明：该菌株为贝莱斯芽孢杆菌(Bacillus velezensis)。该菌株可产生蛋白酶、纤维素酶、嗜铁素和磷酸酯酶等生防相关酶，对高粱叶斑病菌(Alternaria alternata)、烟草黑胫病菌(Phytophthora nicotianae)、石榴干腐病菌(Phomopsis sp.)、油菜菌核病菌(Sclerotinia sclerotiorum)、稻瘟病菌(Magnaporthe oryzae)等5种重要植物病原真菌也有很强的抑菌活性，可作为生防制剂进一步研究开发。
- Abstract:
- One strain with significant antagonistic effect against Colletotrichum capsici was obtained by plate-pairing method from the soil samples of healthy pepper rhizosphere in high incidence of pepper anthracnose fields in Zunyi area of Guizhou province.The results showed that the strain was finally identified as Bacillus velezensis by morphological,physiological and biochemical characteristics,a phylogenetic tree was built combined with 16S rDNA, gyrA gene sequence analysis results.The strain could produce biocontrol-related enzymes such as protease,cellulase,siderophore and phosphatase.It also had strong antifungal activity against 5 important plant pathogenic fungus such as Alternaria alternata, Phytophthora nicotianae,Phomopsis sp.,Sclerotinia sclerotiorum and Magnaporthe oryzae.This strain could be further studied and developed as biocontrol agents.Keywords:Bacillus;biological control;pepper anthracnose
参考文献/References:
[1]杨青,易图永.辣椒炭疽病及其防治研究进展[J].江西农业学报,2009,21(7):107-109.[2]刘荣云.几种杀菌剂对辣椒炭疽病的田间防治效果[J].长江蔬菜，2018(20):77-79.[3]古雁宾.三种杀菌剂对辣椒疫病及炭疽病的田间防效试验[J].农民致富之友，2018(19):164.[4]杨翰明,廖卫琴,高应洪,等.遵义永乐镇辣椒炭疽病的发生与防治对策[J].耕作与栽培，2018(1):53-54.[5]李小霞,肖仲久.贵州省辣椒炭疽病病原菌鉴定及室内毒力测定[J].广东农业科学，2011(17):55-57.[6]罗志威,徐滔明,周芳如,等.一种防治辣椒炭疽病的菌肥生产及其在辣椒种植上的应用[J].安徽农业科学，2015(31):140-141.[7]秦刚,吴庆丽.辣椒炭疽病生防菌筛选及抗菌活性初步研究[J].北方园艺，2012(6):124-128.[8]闫洪雪,刘 露,李 丽,等.PGPR的研究进展及其在农业上的应用[J].黑龙江农业科学,2016(6):148-151.[9]蒋桂芳,宋力.辣椒炭疽病生物防治技术的研究与展望[J].湖北农业科学,2014,53(11):2481-2485.[10]何红.辣椒内生枯草芽孢杆菌(Bacillus subtilis )防病促生作用的研究[D].福州:福建农林大学,2003．[11]戴亚静.香蕉镰刀菌枯萎病生防菌的筛选及其根际定殖研究[D].福州:福建农林大学,2018.[12]李华.地衣芽孢杆菌(Bacillus licheniformis)对番茄灰霉病和辣椒炭疽病生物防治的研究[D].成都:四川农业大学,2005．[13]方中达.植病研究方法(第3版）[M].北京:中国农业出版社,2007.[14]东秀珠,蔡秒英.常见细菌系统鉴定手册[M].北京:科学出版社出版,2001.[15]LANE D J.16S/23S rRNA sequencing.In:Stackebrandt E,goodfellow M (eds) nucleic acid techniques in bacterial systematics[M].New York:Wiley,Chichester,1991.[16]SAITOU N,NEI M.The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution，1987(4):406-425.[17]姜莉莉,陈彦闯,辛明秀.枯草芽孢杆菌在防治植物病害上的应用及研宄进展[J].安徽农学通报,2009,15(7):37-39.[18]秦刚,吴庆丽.辣椒炭疽病生防菌筛选及抗菌活性初步研究[J].北方园艺,2012(6):124-128.[19]肖小露.枯草芽孢杆菌BS193对辣椒疫病的生防作用及其抗菌机制初探[D].福州:福建农林大学,2017.[20]张晓阳.影响炭疽菌附着胞形成的生防菌的筛选、鉴定及菌株C-D6的活性蛋白分离[D].南昌:江西农业大学,2011.[21]伏波.解淀粉芽孢杆菌EDR4对葡萄霜霉病的生防作用研究[D].杨凌:西北农林科技大学,2017.[22]陈欣怡.苹果轮纹病生防解淀粉芽胞杆菌(Bacillus amyloliquefaciens)PG12主要生防机制研究[D].北京:中国农业大学,2016.[23]王卫星,周晓伦,李忠玲,等.CAS平板覆盖法检测氢氧化细菌铁载体[J].微生物学通报,2014,41(8):1692-1697.[24]PIKOVSKAYA R.Mobilization of phosphorus in soil in connection with vital activity of some microbial species[J].Mikrobiologiya,1948,17:362-370.[25]雷蕾.植物病原真菌拮抗菌TG116的鉴定、生物学特性及生防机理研究[D].兰州:西北师范大学,2015.[26]蔡高磊,张凡,欧阳友香,等.贝莱斯芽孢杆菌(Bacillus velezensis)研究进展[J].北方园艺,2018(12):162-167.
相似文献/References:
[1]鲁海菊,王 波,潘柳君,等.深绿木霉P3.9生防菌株固体发酵条件优化筛选[J].北方园艺,2014,38(14):119.
LU Hai-ju,WANG Bo,PAN Liu-jun,et al.Screening on Conditions of Solid Fermentation for Biocontrol Strain P3.9 of Trichoderma atroviride [J].Northern Horticulture,2014,38(23):119.
[2]熊仕俊,孙成龙,施闯,等.番茄青枯病菌拮抗放线菌的筛选及鉴定[J].北方园艺,2014,38(05):114.
XIONG Shi-jun,SUN Cheng-long,SHI Chuang,et al.Screening and Identifying of Antagonistic Actinomycetes Against Ralstonia solancearum[J].Northern Horticulture,2014,38(23):114.
[3]高 宇.茶尺蠖天敌生态学研究进展[J].北方园艺,2014,38(09):203.
GAO Yu.Research Progress on Ecology of Natural Enemies of Tea Geometrid[J].Northern Horticulture,2014,38(23):203.
[4]全桂静.苹果采后病害拮抗细菌的筛选与防腐效果研究[J].北方园艺,2013,37(11):130.
QUAN Gui-jing.Study on Screening of Antagonistic Bacteria for Apple Postharvest Diseases and Biocontrol Efficacy[J].Northern Horticulture,2013,37(23):130.
[5]欧善生,韦荣昌,吴庆华,等.广西凉粉草病虫害种类及防治方法研究[J].北方园艺,2013,37(01):177.
OU Shan-sheng,WEI Rong-chang,WU Qing-hua,et al.Study on Varieties of Diseases and Insect Pests and Control Measures of Mesona chinensis in Guangxi[J].Northern Horticulture,2013,37(23):177.
[6]张玉芹.枯草芽孢杆菌Bs-18菌株分离与鉴定及对根结线虫的防治效果[J].北方园艺,2012,36(01):130.
ZHANG Yu-qin.Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita[J].Northern Horticulture,2012,36(23):130.
[7]穆瑞瑞.土壤微生物在农村植物病害生物防治中的作用分析[J].北方园艺,2013,37(08):194.
MU Rui-rui.Analysis of Soil Microorganism on Biological Control of Plant Diseases in Rural Areas[J].Northern Horticulture,2013,37(23):194.
[8]胡琼,张海松.木霉菌TC对辣椒疫病的防治效果[J].北方园艺,2012,36(03):147.
HU Qiong,ZHANG Hai-song.Effect of Biological Control of Pepper Phytophthora Blight by Trichoderma TC[J].Northern Horticulture,2012,36(23):147.
[9]葛平华,马桂珍,付泓润,等.油菜菌核病的生物防治研究进展[J].北方园艺,2013,37(02):185.
GE Ping-hua,MA Gui-zhen,FU Hong-run,et al.Research Progress of the Biological Control of Sclerotinia sclerotiorum[J].Northern Horticulture,2013,37(23):185.
[10]古丽吉米拉·米吉提,王志英,王 娜,等.木霉菌的生物防治分子机理[J].北方园艺,2013,37(23):206.
MIJITI Gulijimila,WANG Zhi-ying,WANG Na,et al.Molecular Mechanism of Biological Control of Trichoderma spp.[J].Northern Horticulture,2013,37(23):206.
备注/Memo
第一作者简介：程欢欢(1993-)，女，硕士研究生，研究方向为植物病害生物防治。E-mail:513396976 @qq.com.责任作者：丁海霞(1986-)，女，博士，讲师，现主要从事植物病理学和植物病害生物防治等研究工作。E-mail:hxding@gzu.edu.cn.基金项目：贵州大学引进人才科研资助项目(贵大人基合字(2016)42号)；贵州省教育厅青年科技人才成长资助项目(黔教合KY字[2018]101）；贵州省科技厅资助项目(黔科合平台人才[2017]5788)。收稿日期：2019-04-28