[1]安东,车永梅,赵方贵,等.解磷菌3P29促进辣椒生长的生理机制[J].北方园艺,2019,43(22):8-16.[doi:10.11937/bfyy.20191118]
　AN Dong,CHE Yongmei,ZHAO Fanggui,et al.Physiological Mechanism in the Growth Promoting Effect of Phosphate Solubilizing Bacteria 3P29 on Pepper[J].Northern Horticulture,2019,43(22):8-16.[doi:10.11937/bfyy.20191118]
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解磷菌3P29促进辣椒生长的生理机制

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年22 页码: 8-16 栏目: 出版日期: 2019-11-30
Title:
Physiological Mechanism in the Growth Promoting Effect of Phosphate Solubilizing Bacteria 3P29 on Pepper
作者:
安东; 车永梅; 赵方贵; 李雅华; 杨德翠; 刘新
（青岛农业大学 生命科学学院，山东省高校植物生物技术重点实验室，山东 青岛 266109）
Author(s):
AN Dong; CHE Yongmei; ZHAO Fanggui; LI Yahua; YANG Decui; LIU Xin
(Life Science College，Qingdao Agricultural University/Key Lab of Plant Biotechnology in Universities of Shandong Province,Qingdao,Shandong 266109)
关键词:
解磷菌; 辣椒; 生长; 生理机制
Keywords:
phosphate-solubilizing bacteria; pepper; growth; physiological mechanism
DOI:
10.11937/bfyy.20191118
文献标志码:
A
摘要:
解磷菌可以将土壤中植物难以吸收利用的磷转化为可吸收利用的磷，是制造生物肥料的理想微生物原料。以辣椒为试材，检测了解磷菌菌株3P29对辣椒幼苗生长指标以及氮代谢相关酶活性和光合气体交换参数、荧光参数等生理指标的影响，探究3P29促进植株生长的生理机制，以期为3P29的开发利用提供参考依据。结果表明：3P29可以显著提高辣椒幼苗地上部分和地下部分干质量、鲜质量以及茎粗等生长指标，促进其生长。3P29显著提高辣椒叶片氮、磷和钾等营养元素以及可溶性蛋白质含量，提高硝酸还原酶（NR）、谷氨酰胺合成酶（GS）和谷氨酸脱氢酶（GDH）等氮代谢相关酶活性；同时，接种3P29的辣椒幼苗叶绿素含量、光合气体交换参数（Pn，Ci，Gs和E）、荧光参数（ΦPSⅡ、qP、ETR、RC/CS0）以及水分利用效率均显著高于未接种植株，NPQ则低于未接种植株，表明3P29能促进植株对氮、磷和钾等营养元素的吸收，促进氮代谢，提高PSⅠ和PSⅡ活性，改善植株的光合性能，进而促进植株生长。
Abstract:
Phosphate (P)-solubilizing bacteria was the soil-born microorganism,which could convert insoluble P in the soil into plant-available P,and was the promising material for producing biofertilizer.Taking pepper as material,the effects of 3P29 on the growth parameters as well as physiological parameters,such as activities of nitrogen (N) metabolism enzymes,phyotosynthetic gas exchange and fluorescence parameters of pepper seedlings were examined,with the aim to uncover the physiological mechanism in the growth,promoting effect of 3P29 and provide theoretical base for the exploitation of 3P29.The results showed that application of 3P29 increased the shoot diameter,as well as dry and fresh weight of aerial and underground parts of pepper seedlings,prompted its growth3P29 treatment elevated the content of mineral nutrient,such as nitrogen(N),phosphorus(P) and potassium (K),as well as soluble protein in pepper leaves,and enhanced activities of enzymes involved in N metabolism,such as nitrate reductase (NR),glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in pepper roots and leaves.Meanwhile,pepper seedlings treated with 3P29 exhibited increased foliar chlorophyll level,gas exchange parameters (Pn,Ci,Gs and E),fluorescence parameters (ΦPSⅡ,qP,ETR and RC/CS0),as well as water use efficiency compared to those of the untreated control,but showed decrease in NPQ.These results manifested that 3P29 application prompted N metabolism,enhanced PSⅠand PSⅡ activities,elevated photosyethetic capacity,so improved the growth of pepper seedlings.

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备注/Memo

第一作者简介：安东（1994-），男，硕士研究生，研究方向为生物工程。E-mail:49904334@qq.com.责任作者：刘新（1966-），女，博士，教授，研究方向为植物逆境生理和细胞信号转导。E-mail:liuxin6080@126.com.基金项目：山东省重点研发计划资助项目（2017GNC10103）；山东省现代农业产业技术体系创新团队资助项目(SDAIT-25-04)。收稿日期：2019-05-04

更新日期/Last Update: 2019-12-10