[1]李亚洁,孟楠,温志新,等.60Co-γ射线与ARTP复合诱变选育蛹虫草高产菌株[J].北方园艺,2019,43(20):112-116.[doi:10.11937/bfyy.20190993]
　LI Yajie,MENG Nan,WEN Zhixin,et al.Breeding of High Yield Strains of Cordyceps militaris by Combined Mutation of 60Co-γ and ARTP[J].Northern Horticulture,2019,43(20):112-116.[doi:10.11937/bfyy.20190993]
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60Co-γ射线与ARTP复合诱变选育蛹虫草高产菌株

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年20 页码: 112-116 栏目: 出版日期: 2019-10-30
Title:
Breeding of High Yield Strains of Cordyceps militaris by Combined Mutation of 60Co-γ and ARTP
作者:
李亚洁1; 2; 孟楠1; 2; 温志新1; 2; 李学军1; 2; 李树英1; 2; 都兴范1; 2
（1.辽宁省海洋水产科学研究院，辽宁 大连 116023；2.辽宁省农业科学院 大连生物技术研究所，辽宁 大连 116024）
Author(s):
LI Yajie1; 2; MENG Nan1; 2; WEN Zhixin1; 2; LI Xuejun1; 2; LI Shuying1; 2; DU Xingfan1; 2
(1.Liaoning Ocean and Fishery Science Research Institute，Dalian，Liaoning 116023;2.Dalian Biotechnology Research Institute，Liaoning Academy of Agricultural Sciences，Dalian，Liaoning 116024)
关键词:
蛹虫草; 60Co-γ射线; ARTP; 复合诱变
Keywords:
Cordyceps militaris; 60Co-γ ray; ARTP; compound mutagenesis
DOI:
10.11937/bfyy.20190993
文献标志码:
A
摘要:
以实验室保存的蛹虫草菌株（“BY2”）为出发菌株，采用60Co-γ射线与ARTP复合诱变方法对其进行处理，经过初筛、复筛及稳定性试验，研究了诱变菌株的菌落特征及子实体产量，以期获得蛹虫草高产菌种。结果表明：经复合诱变处理，筛选出3株高产突变株，其子实体产量与出发菌株相比提高率分别为32.27%、36.00%和33.87%。通过突变菌株的遗传稳定性及工厂化生产测试，BY2-1122菌株是传代稳定性较好的菌株，其子实体产量明显高于现行生产菌株，增幅达17.3%。研究表明，利用60Co-γ射线与ARTP复合诱变选育蛹虫草，可以获得高产子实体菌株。
Abstract:
A laboratory preserved strain of C.militaris(‘BY2’) was used to be treated by 60Co-γ ray combined with ARTP mutagenesis，After primary screening，rescreening and stability tests，the colony characteristics and fruiting body yield of the mutant strains were studied to obtain high-yield strains of C.militaris.The results showed that three high yield mutants were screened out.Compared with the original strain，the fruit body yield of the high yield mutants was increased by 32.27%,36.00%and 33.87%，respectively.The genetic stability experiment and factory production showed that the mutant strain BY2-1122 was the most stable strain and its fruiting body yield was significantly higher than that of current producing strain，with an increase of 17.3%.Our study showed that the breeding of C.militaris treated by 60Co-γ ray combined with ARTP could obtain the high yield fruiting body strain.

参考文献/References:

[1]郑壮丽,黄春花,梅彩英,等.蛹虫草国内外研究的新进展[J].环境昆虫学报,2011,33(2):225-233.[2]张姝,张永杰,SHRESTHA B,等.冬虫夏草菌和蛹虫草菌的研究现状、问题及展望[J].菌物学报,2013,32(4):577-597.[3]李亚洁,王鹤,孟楠,等.蛹虫草菌种复壮技术的研究[J].食用菌,2006(2):18-19.[4]张俊涛,温志新,李亚洁,等.蛹虫草常用菌种选育技术[J].食用菌,2010(3):20-21.[5]杨玉红,刘芳,康宗利,等.北虫草菌种人工分离的研究[J].中国食用菌,2012(1):11-12.[6]胡中娥,钟国祥,沈爱喜,等.北冬虫夏草组织分离及复壮菌种的试验研究[J].江西农业学报,2013(10):124-125,134.[7]王艳华,徐国华,李剑梅,等.蛹虫草高产优良菌株的选育[J].食用菌,2017(2):24-26.[8]付立忠,吴学谦,魏海龙,等.我国食用菌育种技术应用研究现状与展望[J].食用菌学报,2005,12(3):63-68.[9]李荣杰.微生物诱变育种方法研究进展[J].河北农业科学2009,13(10):73-76,78.[10]刘 燕,杨建松,王露,等.等离子诱变选育柠檬酸高产菌[J].天津科技大学学报,2014,29(1):16-19.[11]卢翠文.北冬虫夏草菌丝体片段诱变育种[J].生物技术,2008,18(5):23-26.[12]李文,赵世光,陈宏伟,等.低能离子束修饰蛹虫草菌株高产虫草素[J].生物工程学报,2009,25(11):1725-1731.[13]周礼红,王仕敏,罗丽美.高产虫草菌素蛹虫草菌株的紫外线诱变选育[J].食用菌,2009(4):20-21.[14]翟景波,滕利荣,黄宝玺.高产蛹虫草菌株的选育[J].中国生物制品学杂志,2011,24(10):1230-1232.[15]贾国军,马振贵,赵凤舞,等.重离子束辐照诱变选育北冬虫夏草高产菌株[J].辐射研究与辐射工艺学报,2017,35(2):26-32.

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备注/Memo

第一作者简介：李亚洁（1964-），女，本科，研究员，研究方向为蛹虫草菌种选育及栽培技术。E-mail:lyj81103392@163.com.基金项目：辽宁省自然科学基金资助项目（20180551243）；现代农业产业技术体系建设专项资助项目（CARS-18-ZJ0504）。收稿日期：2019-04-28

更新日期/Last Update: 2019-11-04