HUANG Xiaojing,NIU Ruimin,SHEN Tian,et al.Effects of Different Fertilization Treatments on Chlorosis of ‘Cabernet Sauvignon’ Grape Leaves in Sandy Soil[J].Northern Horticulture,2019,43(21):25-32.[doi:10.11937/bfyy.20190988]
不同施肥处理对风沙地‘赤霞珠’葡萄叶片黄化的影响
- Title:
- Effects of Different Fertilization Treatments on Chlorosis of ‘Cabernet Sauvignon’ Grape Leaves in Sandy Soil
- 文献标志码:
- A
- 摘要:
- 为矫治‘赤霞珠’葡萄的叶片黄化，以出现黄化的‘赤霞珠’为试验材料，研究叶喷0.1%奥西沐+0.1%萃丽(TR1)、灌根3%萃腾+0.5%萃护一号+0.2%奥西沐(TR2)及叶喷0.1%荷黄铁+0.1%萃丽(TR3)对植株叶片相对叶绿素含量、光合特性、快速叶绿素荧光诱导动力学特性的影响，以期为宁夏贺兰山东麓地区酿酒葡萄叶片黄化的改善建立提供科学依据。结果表明：叶片黄化导致其SPAD、净光合速率(Pn)显著降低，光合作用受到抑制，快速叶绿素荧光诱导曲线变形，PSⅡ最大光化学效率(Fv/Fm)显著下降29.5%；施肥处理可显著增加叶片的SPAD及Pn，并增加叶片对光能的利用效率，线性电子传递速率明显提高；TR3(叶面喷施0.1%荷黄铁+0.1%萃丽)叶片的Pn最高，达到(19.62±0.08)CO2 μmol?m-2?s-1，其SPAD为43.84±0.40，并且其叶片光合原初反应激发能分配最优，光化学效率最高，矫治‘赤霞珠’葡萄叶片黄化的效果较优。
- Abstract:
- To correct the chlorosis of ‘Cabernet Sauvignon’ grape leaves.The ‘Cabernet Sauvignon’ grapevines were used to investigate the effect of different fertilization treatments(spraying 0.1% Auxym+0.1%TRAINER，ripening 3% Revive 4.8 EDDHA+0.5% PHOSPHIT ONE+0.2% Auxym，spraying 0.1% Hehangtie and 0.1% TRAINER) on photosynthetic characteristics of grape leaves under etiolation.The content of chlorophyll，OJIP fluorescence transient，and photosynthetic characteristics were determined.The results showed that photosynthetic characteristics of ‘Cabernet Sauvignon’ grape was inhibited by etiolation.The value of SPAD and Pn (photosynthetic rate) were significantly lower than that of normal plant，the shape of the OJIP curve was clearly changed under etiolation，and leaf maximum photochemical efficiency (Fv/Fm) significantly decreased by 29.5%.In contrast，the fertilization treatments reverted these changes of grape leaves under etiolation.Declined the increasing amplitude of the K points and enhanced photosynthesis rate clearly.Linear electron transfer rate was significantly improved.The effects of TR3 (spraying 0.1% Hehangtie and 0.1% TRAINER) was better.The value of Pn was (19.62±0.08)CO2μmol?m-2?s-1，which was significantly higher than other treatments，and the value of SPAD was 43.84±0.40.Leaves of TR3 had the optimum allocation of excitation energy in photosynthetic primary reaction，the lowest non-photochemical quenching，and the highest photochemical efficiency.The chlorosis of ‘Cabernet Sauvignon’ grape leaves were corrected by spraying 0.1% Hehangtie and 0.1% TRAINER.
参考文献/References:
[1]KOBAYASHI T,NISHIZAWA N K.Iron sensors and signals in response to iron deficiency[J].Plant Science An International Journal of Experimental Plant Biology,2014,224(13):36-43.[2]伊丽米努尔,李宏,杨璐,等.鄯善绿洲灌溉水矿化度对葡萄叶片黄化、干枯的影响[J].浙江农业学报,2015,27(10):1813-1816.[3]查倩,奚晓军,蒋爱丽,等.高温胁迫对葡萄幼树叶绿素荧光特性和抗氧化酶活性的影响[J].植物生理学报,2016,52(4):525-532.[4]尚雁鸿,韩江霞,魏鹏.不同铁配方对葡萄缺铁黄化的防治效果[J].吉林农业科学,2014,39(3):71-73.[5]周龙,刘春燕,董凯向,等.生理性黄化对吐鲁番地区葡萄枝、叶形态学变化的影响[J].新疆农业科学,2018,55(8):1473-1482.[6]刘春燕,周龙,贾舟楫,等.黄化对吐鲁番葡萄叶片光合及叶绿素荧光特性的影响[J].经济林研究,2018,36(2):115-120.[7]熊博,严巧巧,孙国超,等.不同黄化程度对杂柑幼苗生长及光合作用的影响[J].浙江农业学报,2017,29(9):1506-1514.[8]王翠玲,杨晓明,曹孜义.缺铁黄化对葡萄生长及果实品质的影响[J].果树学报,2007(1):26-29.[9]刘士平,郑录庆,田伟,等.高等植物中铁的代谢机制[J].植物生理学报,2011,47(10):967-975.[10]黄建全,聂松青,张娜,等.不同叶面肥对“金手指”葡萄叶片黄化的影响[J].北方园艺,2015(19):161-163.[11]郭爱霞,王延秀,唐健,等.不同复合铁肥对石灰质土壤苹果黄化病的矫正效果[J].甘肃农业大学学报,2018,53(3):76-82.[12]陈城,张福生,彭忱晨,等.不同铁肥对缺铁桃树土壤酶活性与细菌群落结构的影响[J].四川大学学报(自然科学版),2018,55(1):191-196.[13]王光州,韩慧韬,车金鑫,等.不同铁制剂对石灰性土壤条件下猕猴桃缺铁黄化的矫治效果[J].果树学报,2011,28(1):61-65.[14]苏律,宋俊霞,胡同乐,等.铁肥不同施用方式对苹果缺铁黄化病的矫正效果[J].江苏农业科学,2016,44(1):188-189.[15]晁无疾,周敏,张铁强,等.葡萄缺铁性黄化病调查与矫治试验[J].中外葡萄与葡萄酒,2000(2):25-27.[16]鲍士旦.土壤农化分析试验[M].北京:中国农业出版社,2000.[17]李鹏民,高辉远,STRASSER J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.[18]邱念伟,周峰,王颖,等.松树与杨树叶片叶绿素快相荧光动力学特征比较[J].林业科学,2013,49(3):136-143.[19]LPEZ-RAYO S,NADAL P,LUCENA J J.Novel chelating agents for iron,manganese,zinc,and copper mixed fertilization in high pH soil-less cultures[J].Journal of the Science of Food and Agriculture,2016,96(4):1111-1120.[20]王锐.贺兰山东麓土壤特征及其与酿酒葡萄生长品质关系研究[D].杨凌:西北农林科技大学,2016.[21]王锐,李颖,李磊,等.施肥区域对酿酒葡萄生长及品质的影响[J].江苏农业科学,2018,46(13):109-112.[22]苏晓琼,王美月,束胜,等.外源亚精胺对高温胁迫下番茄幼苗快速叶绿素荧光诱导动力学特性的影响[J].园艺学报,2013,40(12):2409-2418.[23]SAIRAM R K,SRIVASTAVA G C.Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress[J].Plant Science,2002,162(6):897-904.[24]黎冰,侍朋宝,栾雪涛,等.硝铵态氮不同配比对葡萄新梢生长和叶片光合特性的影响[J].西北农林科技大学学报(自然科学版),2018,46(2):73-83.[25]何丽斯,苏家乐,刘晓青,等.高山杜鹃叶片叶绿素含量测定及其与SPAD值的关系[J].江苏农业科学,2012,40(11):190-191.[26]张媛,邵建柱,刘兴菊,等.我国板栗光合作用研究进展[J].西北林学院学报,2007(5):53-62.[27]卞凤娥,肖秋红,郝桂梅,等.根施褪黑素对NaCl胁迫下葡萄内源褪黑素及叶绿素荧光特性的影响[J].中国农业科学,2018,51(5):952-963.[28]CHEN H X,LI W J,AN S Z,et al.Characterization of PSII photochemistry and thermostability in salt-treated Rumex leaves[J].Journal of Plant Physiology,2004,161(3):257-264.[29]WEI Z,SUI Z,WANG J,et al.Effects of sodium bicarbonate concentration on growth,photosynthesis,and carbonic anhydrase activity of macroalgae Gracilariopsis lemaneiformis,Gracilaria vermiculophylla,and Gracilaria chouae (Gracilariales,Rhodophyta)[J].Photosynthesis Research,2016,128(3):259-270.[30]王志军,谢宗铭,田又升,等.膜下滴灌水稻黄化叶片光合特性及叶绿素荧光参数分析[J].西北农业学报,2015,24(2):59-65.[31]毕焕改,董绪兵,刘培培,等.CsRCA超表达对高温胁迫下黄瓜幼苗光合作用的影响[J].应用生态学报,2016,27(7):2308-2314.
相似文献/References:
[1]朱玉珍,惠竹梅,张辉.不同施氮水平对‘赤霞珠’葡萄叶片氮素化合物及关键酶活性的影响[J].北方园艺,2012,36(16):142.
ZHU Yu-zhen,XI Zhu-mei,ZHANG Hui.Effect of Different Nitrogen Application Treatments on Nitrogen Compounds Content and Nitrogen Metabolism Key Enzymes Activity in ‘Cabernet Sauvignon’ Grape Leaf[J].Northern Horticulture,2012,36(21):142.
[2]陈德生,刘祥义.杜鹃黄化现象研究[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108036]
CHEN De-sheng,LIU Xiang-yi.Study on Phenomenon of Diagnosze Azalea Chlorosis for Rhododendron arboreum Hort.[J].Northern Horticulture,2011,35(21):0.[doi:10.11937/bfyy.201108036]
[3]党艳青,焦灰敏,王新建.阿拉尔苹果园缺铁失绿诊断及矫治[J].北方园艺,2018,42(09):81.[doi:10.11937/bfyy.20173616]
DANG Yanqing,JIAO Huimin,WANG Xinjian.Diagnosis and Treatment of Iron Deficiency Chlorosis of Apple Achard in Alar[J].Northern Horticulture,2018,42(21):81.[doi:10.11937/bfyy.20173616]
[4]冯国华,冀馨宁,杨静慧,等.不同品种蓝莓植株失绿的叶片和土壤诊断及分析[J].北方园艺,2019,43(09):34.[doi:10.11937/bfyy.20182835]
FENG Guohua,JI Xinning,YANG Jinghui,et al.Diagnosis and Analysis of Leaves and Soils for Plant Chlorosis of Different Blueberry Varieties[J].Northern Horticulture,2019,43(21):34.[doi:10.11937/bfyy.20182835]
[5]杨君,张振文.不同新梢留量对‘赤霞珠’葡萄果实品质的影响[J].北方园艺,2020,44(07):43.[doi:10.11937/bfyy.20192756]
YANG Jun,ZHANG Zhenwen.Effect of Different Shoot Density on the Grape Fruit Quality of ‘Cabernet Sauvignon’[J].Northern Horticulture,2020,44(21):43.[doi:10.11937/bfyy.20192756]
[6]郭帅奇,刘竞择,张艳霞,等.水分胁迫对‘赤霞珠’葡萄不同叶龄叶片糖含量及相关代谢酶活性的影响[J].北方园艺,2021,(12):17.[doi:10.11937/bfyy.20203493]
GUO Shuaiqi,LIU Jingze,ZHANG Yanxia,et al.Effects of Water Stress on Sugar Content and Related Metabolic Enzyme Activities in Leaves of ‘Cabernet Sauvignon’ Grape at Different Leaf Ages[J].Northern Horticulture,2021,(21):17.[doi:10.11937/bfyy.20203493]
[7]马阳历,张玉笑,郭衍银,等.气调过程中O2含量对西兰花叶绿素降解的影响[J].北方园艺,2021,(23):98.[doi:10.11937/bfyy.20212248]
MA Yangli,ZHANG Yuxiao,GUO Yanyin,et al.Effects of O2 Content on the Chlorophyll Degradation in Broccoli Heads Under Controlled Atmospheres[J].Northern Horticulture,2021,(21):98.[doi:10.11937/bfyy.20212248]
备注/Memo
第一作者简介：黄小晶(1992-)，女，宁夏银川人，硕士，研究实习员，研究方向为葡萄与葡萄酒。E-mail：hxj92036wh@163.com.责任作者：陈卫平（1970-），男，宁夏吴忠人，博士，研究员，研究方向为葡萄栽培与生理。E-mail：nature06chen@163.com.基金项目：国家现代农业产业技术体系资助项目(CARS-29);宁夏农林科学院科技创新引导项-青年基金资助项目(NKYQ-18-05)。收稿日期：2019-05-06