HE Chao,KONG Qiong,YUAN Shengyong,et al.Effects of Temperature and Relative Humidity Stress on the Survival and Fecundity of the Melon Fly, Bactrocera cucurbitae (Diptera：Tephritidae)[J].Northern Horticulture,2019,43(15):36-43.[doi:10.11937/bfyy.20184372]
温湿度胁迫对瓜实蝇存活和繁殖特性的影响
- Title:
- Effects of Temperature and Relative Humidity Stress on the Survival and Fecundity of the Melon Fly, Bactrocera cucurbitae (Diptera：Tephritidae)
- 文献标志码:
- A
- 摘要:
- 以瓜实蝇为研究对象，采用生物测定法，通过研究成虫、蛹、卵在不同温度和湿度胁迫下，成虫存活率及寿命、产卵量、卵孵化率、羽化率等生物学特性的变化，以期明确温湿度胁迫对该虫存活和繁殖特性的影响。结果表明：成虫在39～43 ℃下处理1 h，其存活率均随温度的升高而降低，41.5～42.5 ℃是成虫的致死敏感高温区；35 ℃或41 ℃下处理0.5～10.0 d，存活率均随处理时间的延长而降低；成虫在31～41 ℃下驯化1 h后，在43 ℃高温下的存活率随驯化温度的升高先增后减；在43 ℃处理2 h或4 ℃处理3 d后，成虫寿命及单雌产卵量均显著降低；蛹在33～47 ℃下处理4 h，羽化率随温度的升高而降低；卵在41～43 ℃下分别处理15、30、45 min，卵孵化率均随处理时间延长而显著降低。蛹的羽化率随沙土含水量升高而降低；湿度胁迫不同日龄蛹的羽化率差异显著；蛹在沙土含水量0%下处理1～9 d，羽化率随胁迫时间延长而下降；25 ℃下，成虫在RH 0%或RH 100%下处理10～130 h，存活率随处理时间的延长而降低；干旱胁迫下，羽化率随高温或低温处理时间的延长而显著降低。以上研究表明，温、湿度胁迫对瓜实蝇存活和繁殖有显著抑制作用，抑制效果与温、湿度持续时间及胁迫时各虫态龄期密切相关。
- Abstract:
- Bactrocera cucurbitae was used as test insect,the biological characteristics of adult survival rate and longevity,number of eggs laid per female,egg hatching rate and emergence rate were studied after adult,pupae and egg were treated at different temperatures and humidity stresses using bioassay methods,in order to clarify the effects of temperature and humidity stress on the survival and reproduction characteristics of the melon flies.The results showed that the adult were treated at 39-43 ℃ for 1 hour,and the survival rate decreased with the increase of temperature.41.5-42.5 ℃ was the lethal sensitive high temperature area of adults.After treatment at 35 ℃ or 41 ℃ for 0.5-10.0 hours,the survival rate decreased with the prolongation of treatment time.After acclimation for 1 hour at 31-41 ℃,the survival rate of the adult at 43 ℃ increased first and then decreased with the increase of the acclimation temperature.After treatment at 43 ℃ for 2 h or 4 ℃ for 3 days,adult longevity and number of eggs laid per female were significantly reduced.At 33-47 ℃,the emergence rate of pupae decreased with increasing temperature.The eggs were treated at 41-43 ℃ for 15,30 and 45 minutes respectively,the hatching rate of eggs decreased significantly with the prolongation of treatment time.The emergence rate of pupae decreased with the increase of the soil water content.There is also a significant difference in the emergence rate when humidity stress was different for different ages of pupae.The treatment was carried out under drought stress for 1-9 days,and the emergence rate decreased with the prolongation of stress time.At 25 ℃,adults were treated at the relative humidity 0% or 100% for 10-130 hours,and the survival rate decreased with the prolongation of treatment time.Under drought stress,the emergence rate decreased significantly with the prolongation of high temperature or low temperature exposure time.The results showed that temperature and humidity stress had significant inhibitory effects on the survival and reproduction of the melon flies,and the inhibitory effect was closely related to the duration of temperature and humidity and the age of each stage of the melon flies in the stress.
参考文献/References:
[1]汪兴鉴，赵明珠.中国寡鬃实蝇属记述（双翅目：实蝇科）[J].动物分类学报，1989，14(2)：209-219.[2]张亚楠，龚治，牛黎明，等.基于COI基因片段的瓜实蝇遗传多样性分析[J].热带作物学报，2017，38(5)：926-931.[3]张亚楠，牛黎明，周世豪，等.基于微卫星分子标记的中国瓜实蝇不同地理种群遗传分化分析[J].昆虫学报，2018，61(5)：613-619.[4]李磊，马华博，牛黎明，等.生境及气象因子对瓜实蝇雄成虫数量动态的影响[J].环境昆虫学报，2016，38(4)：766-770.[5]张全胜.瓜实蝇生物学特性观察及其防治建议[J].植保技术与推广，2002，22(3)：14-15；[6]赖敏，曾东强，刘晓亮.溴氰菊酯和氯氰菊酯作为产卵驱避剂在瓜实蝇防治中的应用技术研究[J].植物检疫，2016，30(6)：21-26.[7]姜姗，李帅，张彬，等.极端高温对西花蓟马存活、繁殖特性及体内海藻糖、山梨醇含量的影响[J].中国农业科学，2016，49(12)：2310-2321.[8]ZIZZARI Z V,ELLERS J.Effects of exposure to short-term heat stress on male reproductive fitness in a soil arthropod[J].Journal of Insect Physiology,2011,57(3)：421-426.[9]ZHOU Z S,GUO J Y,CHEN H S,et al.Effect of humidity on the development and fecundity of Ophraella communa (Coleoptera：Chrysomelidae)[J].Biocontrol,2010,55(2):313-319.[10]JEJR E,WITZ J A,AWJR H,et al.Survival of pupae of Heliothis virescens and Heliothis zea (Lepidoptera：Noctuidae) at low temperatures[J].Canadian Entomologist,1982,114(4):289-301.[11]李干金，徐显浩，张海亮，等.短时高温暴露对褐飞虱存活和生殖特性的影响[J].中国农业科学，2015，48(9)：1747-1755.[12]罗茵，祝国栋，孙夏，等.高温胁迫对异迟眼蕈蚊与韭菜迟眼蕈蚊的致死作用及后续发育繁殖的影响[J].应用昆虫学报，2017，54(6)：990-998.[13]罗敏，郭建英，周忠实，等.短时低温胁迫对广聚萤叶甲发育和生殖的影响[J].昆虫学报，2011，54(1)：76-82.[14]安志芳，张爱民，刘向东.低温胁迫对灰飞虱种群生长发育与繁殖的影响[J].应用昆虫学报，2011，48(5)：1309-1313.[15]于莹，徐红星，郑许松，等.在高温下干旱胁迫对褐飞虱生态适应性的影响[J].植物保护学报，2013，40(3)：193-199.[16]吴坤君，龚佩瑜.棉铃虫蛹期在极端湿度下的失水动态[J].昆虫学报，2001，44(4)：512-517.[17]WU K,CHEN Y,LI M.Performances of the cotton bollworm,Heliothis armigera (Hubner) at different temperatures and relative humidities[J].Journal of Environmental Sciences,1993,47(2):158-168.[18]黄娜娜，代鹏，符悦冠，等.瓜实蝇耐低温相关生理指标的测定[J].应用昆虫学报，2015，52(3)：694-701.[19]韦淑丹，黄树生，王玉群，等.温度对瓜实蝇实验种群生长发育及生殖的影响研究[J].南方农业学报，2011，42(7)：744-747.[20]刘奎，符悦冠，何声阳，等.瓜实蝇过冷却点的测定[J].中国蔬菜，2011(10)：80-82.[21]李伟东，肖琼，艾洪木，等.瓜实蝇的有效积温及其在中国的年发生代数预测[J].华东昆虫学报，2008，17(3)：188-193.[22]袁盛勇，孔琼，肖春，等.桔小实蝇成虫人工饲料的筛选[J].华中农业大学学报，2006，25(4)：371-374.[23]袁盛勇，肖春，李正跃，等.桔小实蝇实验室饲养技术研究[J].江西农业大学学报，2003，25(4)：577-580.[24]冯晓洁，席国成，刘福顺，等.温湿度和食物对暗黑鳃金龟幼虫存活及生长发育的影响[J].昆虫学报，2017，60(8)：920-927.[25]刘全科，黄长征，陈兵.热驯化对南美斑潜蝇取食、生殖力和后代种群的影响[J].湖北植保，2007(4)：324-328.[26]PIYAPHONGKUL J,PRITCHARD J,BALE J.Effects of acclimation on the thermal tolerance of the brown planthopper Nilaparvata lugens (Stl)[J].Agricultural and Forest Entomology,2014,16(2)：174-183.[27]崔旭红，谢明，万方浩.短时高温暴露对B型烟粉虱和温室白粉虱存活以及生殖适应性的影响[J].中国农业科学，2008，41(2)：424-430.[28]任璐，陆永跃，曾玲.沙土含水量对桔小实蝇蛹存活的影响[J].华南农业大学学报，2007，28(1)：63-66.[29]林进添，梁广文，曾玲，等.土壤含水量对桔小实蝇蛹期存活的影响[J].昆虫知识，2005，42(4)：416-418.[30]郭慧芳，陈长琨.高温胁迫对雄性棉铃虫生殖力的影响[J].南京农业大学学报，2000，23(1)：30-33.
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备注/Memo
第一作者简介：何超（1979-），男，博士，讲师，现主要从事农业昆虫及害虫综合治理等研究工作。E-mail：hechao1022@163.com.责任作者：袁盛勇（1975-），男，硕士，副教授，现主要从事农业昆虫及害虫综合治理等研究工作。E-mail：ysy9069@163.com.基金项目：云南省应用基础研究计划面上资助项目(2016FB069)；红河学院植物保护硕士点建设资助项目。收稿日期：2019-03-28