DONG Dong,SHI Cuie,YAN Shoubao,et al.Effects of Elevated Atmospheric CO2 and O3 on Soil Enzyme Activities and Microbial Biomass of Phenanthrene Polluted Soil[J].Northern Horticulture,2019,43(12):92-99.[doi:10.11937/bfyy.20184335]
大气CO2、O3升高对菲污染土壤酶活性和微生物量的影响
- Title:
- Effects of Elevated Atmospheric CO2 and O3 on Soil Enzyme Activities and Microbial Biomass of Phenanthrene Polluted Soil
- 文献标志码:
- A
- 摘要:
- 菲（PHE）属多环芳烃类（PAHs）有机污染物，可通过多种途径进入土壤并逐步累积，潜在威胁着土壤的环境质量和生态安全。该研究利用开顶式气室（OTC）模拟CO2或O3浓度升高以及CO2和O3浓度共同升高对菲污染土壤（添加菲）的理化性质、微生物量和酶活性的影响。结果表明：大气中CO2浓度升高不同程度地提高了菲污染土壤的硝态氮（NN）、铵态氮（AN）、总磷（TP）、总碳（TC）、可溶性有机碳（DOC）、可溶性有机氮（DON）等理化指标以及微生物量碳（MBC）和微生物量氮（MBN）。蛋白酶（PRA）、蔗糖酶（SA）、脲酶（UA）、多酚氧化酶（POA）等土壤酶活性也有不同程度的升高；O3浓度升高则降低了菲污染土壤上述理化指标和酶活性；而CO2和O3共同升高能够缓解O3对菲污染土壤理化指标和酶活性的抑制效应，也缓释了CO2对土壤微生物量和酶活性的刺激效应；正常大气条件下，菲污染增加了上述土壤理化指标和酶活性。在相同的CO2或O3暴露环境下，菲污染只是改变了土壤的酶活性和微生物量，而变化趋势基本不发生改变。
- Abstract:
- Phenanthrene(PHE)，a representative of polycyclic aromatic hydrocarbons(PAHs)，is gradually accumulating with superposed manner at a low dose in the soil，and will cause a potential threat to soil ecological safety.The effects of elevated CO2，O3 or CO2 and O3 on soil physicochemical properties，microbial biomass and soil enzyme activities of phenanthrene polluted soils were investigated under the simulated condition in the lab.The results showed that，the elevated CO2 increased the contents of physicochemical indices such as NO-3-N (NN)，NH+4-N (AN)，total phosphorus (TP)，total carbon (TC)，dissolved organic carbon (DOC)，and dissolved organic nitrogen (DON).Moreover，the contents of microbial biomass carbon (MBC)，and microbial biomass nitrogen (MBN) were also increased.Soil enzyme activities such as protease (PRA)，sucrase (SA)，urease (UA)，phenol oxidase (POA) also increased in varying degrees.However，the elevated O3 reduced the contents of physicochemical indices and soil enzyme activities to some extent.The elevated CO2 and O3 alleviated the inhibition of the physicochemical indices and soil enzyme activities of phenanthrene polluted soil by the elevation of O3，and limited the activation by the elevation of CO2.Under ambient atmosphere，phenanthrene polluted increased the contents of physicochemical indices and soil enzyme activities to some extent.The values of soil enzyme activities and microbial biomass of phenanthrene polluted soil were changed merely at the same CO2 or/and O3 concentrations,while the changing trend remains unchanged.
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备注/Memo
第一作者简介：董冬（1985-），男，博士，讲师，现主要从事园林植物及城市生态环境等研究工作。E-mail:hfdondong@163.com.责任作者：施翠娥（1974-），女，博士，副教授，现主要从事环境微生物学等研究工作。E-mail:shicuie1025@163.com.基金项目：国家自然科学基金资助项目（31501461）；安徽省高校优秀拔尖人才培育资助项目（gxfx2017091）；安徽省高校优秀中青年骨干人才国内外访学研修重点资助项目（gxfxZD2016205）。收稿日期：2019-03-14