MA Xiaoying,LYU Litao,CAO Jun,et al.Study on the Capillary Electrophoresis Fingerprint of Morchella[J].Northern Horticulture,2019,43(12):131-138.[doi:10.11937/bfyy.20184257]
羊肚菌毛细管电泳指纹图谱的研究
- Title:
- Study on the Capillary Electrophoresis Fingerprint of Morchella
- Keywords:
- capillary electrophoresis; Morchella; fingerprint; cluster analysis
- 文献标志码:
- A
- 摘要:
- 以8种不同地区采集的羊肚菌为试材，采用分离柱为未涂层石英毛细管(50 μm×48 cm)；运行缓冲液10 mmol?L-1硼砂缓冲液(pH 9.21)；10 mmol?L-1硼酸缓冲液；〖JP3〗检测波长214 nm；分离电压20 kV；温度 25 ℃；压力0.5 psi进样10 s，运行时间为30 min的电泳条件，对其进行高效毛细管电泳检测，建立了羊肚菌的高效毛细管指纹图谱，在方法学验证的基础上,建立了12个共有峰的羊肚菌指纹图谱。以10号峰为菌株指纹图谱的参照峰,通过相似度评价、聚类分析和主成分分析对8种羊肚菌进行质量评价和鉴别，以期建立毛细管电泳技术的羊肚菌指纹图谱的质量控制方法。结果表明：该研究结果与其它分子鉴定方法对照结果一致。此方法样品处理简单，色谱条件容易实现，未使用有毒有害的溶剂，对环境无害，稳定性和重现性好；适用于羊肚菌真伪、品质的鉴别和控制。
- Abstract:
- The Morchella collected from 8 different regions was used as the test material.The separation column was uncoated quartz capillary (50 μm×48 cm)；the running buffer 10 mmol?L-1 borax buffer (pH 9.21)；10 mmol?L-1 boric acid buffer detection wavelength 214 nm；separation voltage 20 kV；temperature 25 ℃；pressure 0.5 psi injection 10 seconds，running time 30 minutes electrophoresis conditions，in order to establish a quality control method based on capillary electrophoresis technology of Morchella fingerprint.On the basis of method validation，the fingerprints of more than 12 common peaks were established.The No.10 peak was used as the reference peak of the fingerprint of the strain，and the quality evaluation and identification of 8 species of Morchella were carried out by similarity evaluation，cluster analysis and principal component analysis.The results showed that the results of this study were consistent with other molecular identification methods.The method had simple sample processing，easy to realize chromatographic conditions，no toxic and harmful solvent，no harm to the environment，good stability and reproducibility，and was suitable for the identification and control of the quality of the morel.
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备注/Memo
第一作者简介：马晓颖（1985-），女，辽宁沈阳人,硕士，助理研究员，现主要从事微生物资源等研究工作。E-mail:mxy851001@126.com.责任作者：王红（1983-），女，山东莒县人，硕士，助理研究员，现主要从事珍稀食用菌资源收集和评价及保育等研究工作。E-mail：wang45852676@126.com.基金项目：沈阳市应用基础研究资助项目（17-231-1-08）；辽宁省自然科学基金资助项目（201602419）；辽宁省农业攻关及产业化资助项目（2017201005）。收稿日期：2019-03-07