DING Lan,GENG Jinpeng,QIN Lei,et al.Mutagenic Effects of Proton and Space Radiation on Antirrhinum majus[J].Northern Horticulture,2019,43(15):82-90.[doi:10.11937/bfyy.20184196]
质子和太空辐射对金鱼草的诱变效应
- Title:
- Mutagenic Effects of Proton and Space Radiation on Antirrhinum majus
- Keywords:
- space mutagenesis; proton irradiation; Antirrhinum majus; Del gene; Rosea gene
- 文献标志码:
- A
- 摘要:
- 以金鱼草为试材，采用太空诱变和质子辐射的方式，研究了辐射对金鱼草表观性状和分子水平的变化，以生物学统计的方法对金鱼草发芽率、株高等数据进行分析。用RAPD和Qpcrd 方法对金鱼草基因组DNA和花青素调控基因Del和Rosea的表达量进行研究。结果表明：2种诱变方式使红色金鱼草发芽率显著降低，黄色金鱼草发芽率基本不变；对株高影响不显著；叶片由对照的矩圆状变为太空诱变后的长椭圆形和质子辐射的披针状；RAPD结果显示太空诱变后产生的多态性比率大于质子辐射，黄色金鱼草的多态性比率稍高于红色金鱼草；qPCR结果显示Del基因和Rosea基因在红色金鱼草中的表达量太空诱变和质子辐射后都高于对照，且太空诱变对红色金鱼草基因的相对表达量高于质子辐射，Del基因和Rosea基因在辐射后黄色鱼草中的表达量都低于对照。
- Abstract:
- The effects of space mutation and proton radiation on the apparent properties and molecular level of Antirrhinum majus were studied.The germination rate and plant height of Antirrhinum majus were analyzed by biological statistics.RAPD and Qpcr methods were used to study the expression of genomic DNA and anthocyanin regulatory genes Del and Rosea in Antirrhinum majus.The results showed that the two mutagenesis methods significantly reduced the germination rate of red Antirrhinum majus,while the germination rate of yellow Antirrhinum majus remained basically unchanged,and had no significant effect on plant height.The blades changed from the control rectangular circle to the long oval shape and the Antirrhinum majus shape of proton radiation after space mutation.The results showed that the rate of polymorphism produced by space mutation was higher than that of proton radiation,and the polymorphism rate of yellow croaker grass was slightly higher than that of red Antirrhinum majus.The results of qPCR showed that the expression levels of Del gene and Rosea gene were higher than those of the control after space mutation and proton radiation,and the relative expression of Del gene and Rosea gene was higher than that of proton radiation.The expression levels of Del gene and Rosea gene were lower than those of the control after space mutation.
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备注/Memo
第一作者简介：丁兰（1994-），女，硕士研究生，研究方向为辐照植物育种。E-mail：1176413164@qq.com.责任作者：曹天光（1972-），男，博士，副研究员，研究方向为辐照生物学。E-mail：caotg2002@163.com.基金项目：河北省高等学校科学技术研究资助项目（ZD2017023）。收稿日期：2019-03-11