DENG Chao,DENG Jie,HUANG Meijuan,et al.Effect of PGPR Strain Containing ACC Deaminase on Post-harvest Physiological Characteristics of Cut Lily ‘Belonica’[J].Northern Horticulture,2019,43(12):63-67.[doi:10.11937/bfyy.20184179]
含ACC脱氨酶的植物根际促生细菌菌株对重瓣百合‘Belonica’采后品质及生理特性的影响
- Title:
- Effect of PGPR Strain Containing ACC Deaminase on Post-harvest Physiological Characteristics of Cut Lily ‘Belonica’
- 关键词:
- 重瓣百合‘Belonica’; ACC脱氨酶; PGPR菌株; 生理特性; 切花保鲜
- Keywords:
- double-flower lily ‘Belonica’; ACC deaminase-containing; PGPR; physiological characteristics; freshness-keeping for cutting flowers
- 文献标志码:
- A
- 摘要:
- 以东方型重瓣百合‘Belonica’为试材，栽培过程中分别施入含ACC脱氨酶的不同PGPR菌株，以清水为对照，百合采切后均分别插入清水中，通过对其相关生理指标进行测定和分析，以探讨施用含ACC脱氨酶的不同PGPR菌株对重瓣百合切花采后品质的影响。结果表明：超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、可溶性蛋白质含量均呈先升后降的趋势；丙二醛（MDA）含量、脯氨酸（Pro）含量总体呈上升趋势，同时发现施用含ACC脱氨酶的不同PGPR菌株后处理在一定程度上均减缓了丙二醛的产生。综合比较发现，菌株BG对重瓣百合切花的延缓保鲜效果最为明显，不仅能较好地维持切花的外观观赏品质，而且能有效延缓切花的衰老进程。
- Abstract:
- In this study，oriental double-flower lily named ‘Belonica’ was used as experimental material.Strain of ACC deaminase-containing PGPR was fertilized during cultivating of the double-flower lily.Under contrast of clear water by putting cutting lily into，related physiological indices were tested and analyzed，and the effect of cutting lily with fertilizing different strains of ACC deaminase-containing PGPR could be explored accordingly.The results showed that activities of SOD，POD and content of soluble protein were firstly increased but then decreased.Contents of malondialdehyde (MDA) and proline (Pro) were generally on the rise.Meanwhile，it was found that the production of MDA was slowed down in a certain extent during the post-treatment to double-lily with fertilizing different ACC deaminase-containing PGPR.Comprehensive comparison discovered that the effect of the strain BG on the preservation of double lily cutting flowers was the most obvious.Strain BG not only maintained the ornamental quality of cutting flowers，but also postponed its aging process effectively.
参考文献/References:
[1]陈榕生.球根花卉[M].福州:福建科学技术出版社,2001.[2]包满珠.花卉学[M].2版.北京:中国农业出版社,2003.[3]GILCK B R，PENROSE D M，LI J.A model for the lowering of plant ethylene concentrations by plantgrowth-promoting bacteria[J].Theory in Biology，1998,190:63-68.[4]LI J，OVABIN D H，CHARLES T C，et al.An ACC deaminase minus mutant of Enterobacter cloacae UM4 no longer promotes root elongation[J].Current Microbiology,2000，41:101-105.[5]PENROSE D M，GLICK B R.Levels of ACC and related compounds in exudates and extracts of canoda seeds treated with ACC deaminase-containing plant growth-promoting bacteria[J].Canadian Journal of Microbiology，2001，47:368-372.[6]MA W，PENROSE D M，GLICK B R.Strategies used by rhizobia to lower plant ethylene levels and increase nodulation[J].Canadian Journal of Microbiology,2002,48(11):947-954.[7]HONTZEAS N，SALEH S S，GLICK B R.Changes in gene expression in canola roots induced by ACC deaminase containing plant growth promoting bacteria[J].Molecular Plant Microbe Interact，2004(8):865-871.[8]葛欣.香石竹抗衰老遗传转化主要影响因素的研究[D].哈尔滨:东北农业大学,2000.[9]张琳,夏宜平,杜方,等迪.乙烯与百合切花瓶插寿命的关系研究进展[J].园艺学报,2013,40(9):1826-1836.[10]张薇,张慧,谷祝平,等.九种花衰老原因的研究[J].植物学报,1991(6):29-36.[11]张华云,王善广.鲜切花瓣采后衰老生理机制[J].天津农林科技,2002(10):19-20.[12]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.[13]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004.[14]林香.百合切花采后衰老相关机理的研究[D].福州:福建农林大学,2007.[15]李金枝.百合切花保鲜和延衰机理的研究[D].武汉:华中师范大学,2004.[16]张华峰,张宾,陈天华,等.乙烯生物合成基因工程在果蔬保鲜中的应用[J].生物技术,2005(6):80-82.[17]SILVA J A，COSTA T S D，LUCCHETTA L，et al.Characterization of ripening be havior in transgenic melons expressing an antisense 1-amin cycle propane-1-carboxylate (ACC) oxidase gene from apple[J].Postharvest Biology and Technology，2004，32:263-268.[18]BELIMOV A A，DODD I C，SAFRANOVA V I，et al.Pseudo monas brassicaca rum strain AM3 containing1-amin cycle propane-1-Carboxyl terminated amine-nase can show both pathogenic and growth-promoting properties in its in teraction with tomato[J].Journl of Exper Imental Botany，2007，58(6):1485-1495.[19]李昌宁,李政璇,曹全熙,等.5株植物根际促生菌对紫花苜蓿生长和品质的影响[J].草原与草坪,2018,38(3):29-34.[20]吴兴兴,吴毅歆,赵正龙,等.4个PGPR菌株拌种对干旱条件下蚕豆生长及产量的影响[J].干旱区研究,2012,29(2):203-207.
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备注/Memo
第一作者简介：邓超（1983-），陕西西安人，硕士，讲师，现主要从事园林植物等研究工作。E-mail:1302988212@qq.com.责任作者：黄海泉（1974-），江西南昌人，博士，教授，现主要从事园林植物等研究工作。E-mail:haiquanl@163.com.基金项目：云南省中青年学术和技术带头人培养资助项目（2015HB046）；国家林业局西南风景园林工程技术研究中心建设资助项目（09Z0066）；云南省园林植物与观赏园艺省级重点学科及云南省高校园林植物与观赏园艺重点实验室资助项目（50097401）。收稿日期：2019-02-18