Aerguli JAMAHATE,Reym MAMUT.Molecular Identification and Phylogenetic Analysis of Lecidea tessellata[J].Northern Horticulture,2019,43(14):40-47.[doi:10.11937/bfyy.20183887]
方斑网衣的分子鉴定和系统发育学分析
- Title:
- Molecular Identification and Phylogenetic Analysis of Lecidea tessellata
- Keywords:
- lichen; Lecidea tessellata; systematics; phylogeny; Xinjiang
- 文献标志码:
- A
- 摘要:
- 为鉴定方斑网衣中的隐存种，以新疆地区的方斑网衣为试验材料，用分子系统发育学方法，研究了方斑网衣核糖体DNA内转录间隔区（internal transcribed spacer,ITS）碱基组成信息、变异位点以及简约信息位点、种内的遗传距离、遗传距离聚类情况、ITS位点系统发育树分析以及基于条形码间隔自动检索鉴定方斑网衣隐存种类。结果表明：方斑网衣ITS基因片段长度为528 bp，其中62个为变异位点、43个为简约位点、460个为保守位点、G+C碱基组成占总含量的56.1%、G+C含量高于A+T含量、单倍型多样性（haplotype (gene) diversity,Hd）为0.993、核酸多样性（nucleotide diversity,Pi）为0.048 76；基于遗传距离构建的聚类树同系统发育树分支聚类情况一致，分为2个大分支；通过条形码自动检索法鉴定出方斑网衣可为2个种。通过综合分析鉴定出方斑网衣2个隐存种即方斑网衣原种（Lecidea tessellata var.tessellata）和方斑网衣蓝变种（Lecidea tessellata var.caesia）。
- Abstract:
- In order to indentify cryptic species within Lecidea tessellata,the specimensof Lecidea tessellatadistributed in Xinjiang were seleted as materials.The internal transcribed spacer (ITS) of L.tessellata was amplified specifically to be used for molecular analyses.The cluter analysis of the genetic distances,automatic barcode gap discovery and molecular phylogenies were studied.The results showed that the length of ITS gene fragment was 528 bp,including 62 variation sites,43 reduced sites and 460 conserved sites.The G+C base composition accounted for 56.1% of the total content,and G+C content was higher than A+T content.Haplotype (gene) diversity (Hd) was 0.993,and nucleotide diversity (Pi) was 0.048 76.The topologies of the molecular phylogenies and cluter based on genetic distances were coincident which fell into two clades,and they were identified as two species by the automatic barcode gap discovery approach.The results indicated that there were two species which identified Lecidea tessellata var. tessellata and Lecidea tessellata var.caesia.
参考文献/References:
[1]阿不都拉?阿巴斯,艾尼瓦尔?吐米尔.新疆地衣研究概述[J].菌物研究,2018,16(1):1-9.[2]KIRK P M,CANNON P F,DAVID J C,et al.Ainsworth & Bisby’s Dictionary of Fungi[M].Wallingford:CAB International,2008.[3]ZAHLBRUCKNER A.Catalogus Lichenum Universalis[M].New York:Johnson Reprint,1932：337-338.[4]HERTEL H.Revision einiger calciphiler formenkreiseder flechtengattung Lecidea[J].Beiheft Nova Hedwig,1967,24:1-155.[5]HOWELL G M,SUSANA E,MARK R D,et al.Raman spectroscopy of rock biodeterioration by the Lichen Lecidea tessellata Flrke in a desert environment,Utah,USA[M].London:Kluwer Academic Publisher,2004,229-249.[6]UPRETI D K,NAYAKA S,ANDREEV M P.Notes on some species of the lichen genus Lecidea from India[J].Mycotaxon,2006,95:323-330.[7]ERIN A T.Field guide to the lichens of white rocks[M].Colorado:University Press of Colorado,2016：72-75.[8]LAMB I M.Index nominum lichenum[M].Wallingford:CAB International,1963：1-80.[9]阿不都拉?阿巴斯,吴继农,姜彦成.新疆地衣植物研究历史回顾[J].干旱区研究,1996(2):1-19.[10]艾尼瓦尔?吐米尔,阿不都拉?阿巴斯.乌鲁木齐南部山区岩面生地衣群落特征的研究[J].菌物学报,2009,28(2):178-188.[11]张璐璐.中国西部地区网衣及其相关属地衣的初步研究[D].聊城:山东师范大学,2012.[12]胡玲.中国网衣科(Lecideaceae)地衣的分类学研究[D].聊城:山东师范大学,2015.[13]GARDES M,BRUNS T D.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J].Mol Ecol,1993（2）:113-118.[14]WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M].London:Academic Press,1990,315-322.[15]KUMAR S,STECHER G，TAMURA K.MEGA7:Molecular genetics analysis version 7.0 for bigger datasets[J].Molecular Biology and Evolution,2016,33:1870-1874.[16]PUILLANDRE N,LAMBERT A,BROUILLET S,et al.ABGD,automatic barcode gap discovery for primary species delimitation[J].Molecular Ecology,2012,21(8):1864-1877.[17]柴华,沙伟.地衣的ITS序列系统发育分析和DNA条形码的初探[J].生物技术,2011,21(1):1-4.[18]柴华.适用于地衣的DNA条形码片段的筛选[D].齐齐哈尔:齐齐哈尔大学,2011.[19]PENG J L,WANG X Z,WANG D,et al.Application of DNA barcoding based on the mitochondrial COI gene sequences in classification of cluter(pisces:cyprinidae)[J].Acta Hydrobiologica Sinioca,2009,33(2):271-276.[20]郑方圆.DNA条形码技术在石耳属地衣分类中的应用[D].哈尔滨:哈尔滨工业大学,2011.[21]HEBERT P D N,CYWINSKA A,BALL S L,et al.Biological identification through DNA barcodes[J].Proceedings of the Royal Society of London,2003,270:313-321.
相似文献/References:
[1]叶嘉,张浩,刘文霞,等.武安国家森林公园几种树生叶状地衣显微结构观察[J].北方园艺,2012,36(06):57.
YE Jia,ZHANG Hao,LIU Wen-xia,et al.The Microscopic Observation of Several Leafy Lichens on Trees of Wu’an National Forest Park[J].Northern Horticulture,2012,36(14):57.
备注/Memo
第一作者简介：阿尔古丽?加玛哈特（1992-），女，硕士研究生，研究方向为植物学分类学。E-mail:1019465928@qq.com.责任作者：热衣木?马木提（1978-），博士,副教授,现主要从事地衣资源调查等研究工作。E-mail:reyim_mamut@xju.edu.cn.基金项目：国家自然科学基金资助项目（31670023,31760052）。 收稿日期：2019-02-14