[1]徐仕英,李映雪,吴忧,等.有性三倍体杂交兰‘黄荷兰’的快速繁殖[J].北方园艺,2019,43(09):69-75.[doi:10.11937/bfyy.20183835]
　XU Shiying,LI Yingxue,WU You,et al.Micropropagation of Sexual Triploid Hybrid Cymbidium ‘Huanghe’[J].Northern Horticulture,2019,43(09):69-75.[doi:10.11937/bfyy.20183835]
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有性三倍体杂交兰‘黄荷兰’的快速繁殖

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年09 页码: 69-75 栏目: 出版日期: 2019-05-15
Title:
Micropropagation of Sexual Triploid Hybrid Cymbidium ‘Huanghe’
作者:
徐仕英1; 李映雪1; 吴忧1; 朱娇1; 郭和蓉1; 2; 曾瑞珍1; 2
（1.华南农业大学 林学与风景园林学院，广东 广州 510642；2.华南农业大学 广东省植物分子育种重点实验室，广东 广州 510642）
Author(s):
XU Shiying1; LI Yingxue1; WU You1; ZHU Jiao1; GUO Herong1; 2; ZENG Ruizhen1; 2
(1.College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642；2.Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou,Guangdong 510642)
关键词:
杂交兰; 有性多倍体; 根状茎; 快速繁殖
Keywords:
hybrid Cymbidium; sexual polyploid; rhizome; micropropagation
DOI:
10.11937/bfyy.20183835
文献标志码:
A
摘要:
以‘黄荷兰’试管苗为试材，采用组织培养快速繁殖方法，研究了影响有性三倍体杂交兰‘黄荷兰’根状茎诱导、增殖、分化、生根壮苗和试管苗移栽的因素，以期建立‘黄荷兰’种苗工厂化生产技术体系。结果表明：横切茎尖的起始脱分化时间早于纵切，诱导率高于纵切。切割长度、外源激素、光照强度和有机附加物对根状茎的增殖影响显著，将根状茎切割成长0.50 cm接种到MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+土豆80.00 g?L-1+蔗糖30.00 g?L-1+卡拉粉7.00 g?L-1+AC 0.30 g?L-1培养基中培养40 d，根状茎增殖系数为3.18。外源激素对根状茎的分化影响显著，将根状茎掰成长1.00 cm接入MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+蔗糖20.00 g?L-1+卡拉粉7.00 g?L-1+AC 0.02 g?L-1培养基中培养40 d，芽分化率和苗分化率分别为60.00%和57.50%。将4 cm高的苗转入1/2MS+6-BA 0.10 mg?L-1+NAA 0.50 mg?L-1+蔗糖20.00 g?L-1+卡拉粉7.00 g?L-1+AC 0.50 g?L-1中培养50 d，平均株高和根数分别为7.32 cm和3.56条。4月和10月用水草移栽，试管苗成活率分别为98.67%和99.67%。上述研究结果说明，利用组织培养快速繁殖技术工厂化生产‘黄荷兰’种苗是可行的。
Abstract:
In order to establish the technical system of micropropagation of sexual triploid hybrid Cymbidium ‘Huanghe’,the test-tube seedlings were employed to investigate the factors influencing on induction,proliferation and differentiation of rhizome,seedling strengthening and transplantation by using of tissue culture rapid propagation method.The results showed that the initial dedifferentiation time of shoot tip with transverse cutting was earlier and the induction rate was higher than that with longitudinal cutting.The length of rhizome,combination of exogenous hormone,light intensity and organic additives had significant effects on rhizome proliferation,and when rhizomes with 0.50 cm in length were inoculated on MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+potato 80.00 g?L-1+sucrose 30.00 g?L-1+AC 0.30 g?L-1 and cultured at 26 ℃ under 1 000 lx light intensity for 40 days,the proliferation coefficient was the highest (3.18).Combination of exogenous hormone significantly affected rhizome differentiation,and when rhizomes in 1.00 cm length were inoculated on MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+sucrose 20.00 g?L-1+AC 0.02 g?L-1 and cultured at 26 ℃ under 2 000 lx light intensity for 40 days,the bud and seedling differentiation rates were 60.00% and 57.50%,respectively.When seedlings with 4.0 cm high were inoculated on 1/2MS+6-BA 0.10 mg?L-1+NAA 0.50 mg?L-1+sucrose 20.00 g?L-1+AC 0.50 g?L-1 and cultured at 26 ℃ under 2 000 lx light intensity for 50 days,the average seedling height and mean number of root were 7.32 cm and 3.56,respectively.The transplantation survival rate of seedlings in April and October was 98.67% and 99.67%,respectively by using of moss as culture substance.The above results demonstrate that it was feasible to produce seedlings of Cymbidium ‘Huanghe’ by the use of tissue culture rapid propagation technology.

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备注/Memo

第一作者简介：徐仕英（1993-），女，硕士研究生，研究方向为花卉组培快繁。E-mail：1512402834@qq.com.责任作者：曾瑞珍（1971-），女，硕士，副研究员，硕士生导师，现主要从事花卉遗传育种等研究工作。E-mail：zengrz@scau.edu.cn.基金项目：广东省科技计划资助项目（2015A020209101，2017B020201010）；广州市科技计划项目产学研协同创新重大专项资助项目（201604020065）；广州市农业局资助项目（1610519，1710212）；清远市科技计划资助项目（2017A005）。收稿日期：2019-01-15

更新日期/Last Update: 2019-05-31