[1]高耀辉,马斌,肖凤洁,等.CRISPR/Cas9系统在园林植物中的研究展望[J].北方园艺,2019,43(15):133-140.[doi:10.11937/bfyy.20183493]
　GAO Yaohui,MA Bin,XIAO Fengjie,et al.Prospect of Research on CRISPR/Cas9 System in Garden Plants[J].Northern Horticulture,2019,43(15):133-140.[doi:10.11937/bfyy.20183493]

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CRISPR/Cas9系统在园林植物中的研究展望

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年15 页码: 133-140 栏目: 出版日期: 2019-08-15

Title:: Prospect of Research on CRISPR/Cas9 System in Garden Plants

作者:: 高耀辉; 马斌; 肖凤洁; 魏光普; （内蒙古科技大学建筑学院，内蒙古包头 014010）

Author(s):: GAO Yaohui; MA Bin; XIAO Fengjie; WEI Guangpu; (Architecture School，Inner Mongolia University of Science and Technology，Baotou，Inner Mongolia 014010)

关键词:: CRISPR/Cas9; 园林植物; 基因组; 育种; 前景

Keywords:: CRISPR/Cas9; garden plants; genome; breeding; prospect

DOI:: 10.11937/bfyy.20183493

文献标志码:: A

摘要:: CRISPR/Cas9系统是近年来迅速发展起来的一种新型基因编辑技术，作为一种高效的育种研究技术已在多种植物中实现了定点突变，具有广阔的应用前景。园林植物观赏性状的改良对丰富园林景观具有重要的作用，但是园林植物的遗传背景复杂，严重制约了基因编辑技术的应用。该文介绍了CRISPR/Cas9系统的基本原理及在植物中的研究进展，总结了CRISPR/Cas9系统针对不同类型植物适合的筛选方法及目前存在的问题，并展望了该技术在园林植物育种中的发展前景，以期能够高效利用CRISPR/Cas9系统改良无基因组参考的植物，快速获得观赏价值高的新品种。

Abstract:: CRISPR/Cas9 technology is a new gene editing technology that has been rapidly developed in recent years.As an efficient breeding research technology，it has achieved site-directed mutation in a variety of plants and has broad application prospects.The improvement of ornamental traits of garden plants played an important role in enriching the landscape.However，the genetic background of garden plants which seriously restricted the application of gene editing technology was complex.Here we introduced the basic principles of CRISPR/Cas9 technology and its research progress in plants.The screening methods and current problems of CRISPR/Cas9 system for different plants were summarized.The prospects of this system in garden plant breeding were expected to improve plants without genome reference efficiently and obtain new varieties with high ornamental value quickly.

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备注/Memo

第一作者简介：高耀辉（1989-），女，博士，讲师，研究方向为园林植物生态修复。E-mail:yaohui_gao@163.com.责任作者：魏光普（1985-），男，博士，讲师，研究方向为生态修复。E-mail:weigp2007@163.com.基金项目：内蒙古自治区自然科学基金资助项目（2017MS(LH)0305）；内蒙古自治区研究生（博士重点）创新基金资助项目（B20171012904Z）；内蒙古科技大学创新启动基金资助项目（2017QDL-S05）。收稿日期：2019-03-19

更新日期/Last Update: 2019-08-19