[1]刘海莉,杨蕾蕾,辛苗苗,等.苹果MdLsi1基因的克隆及生物信息学分析[J].北方园艺,2019,43(09):27-33.[doi:10.11937/bfyy.20183232]
　LIU Haili,YANG Leilei,XIN Miaomiao,et al.Cloning and Bioinformatic Analysis of MdLsi1 Gene in Apple (Malus domestica Borkh.)[J].Northern Horticulture,2019,43(09):27-33.[doi:10.11937/bfyy.20183232]

点击复制

苹果MdLsi1基因的克隆及生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年09 页码: 27-33 栏目: 出版日期: 2019-05-15

Title:: Cloning and Bioinformatic Analysis of MdLsi1 Gene in Apple (Malus domestica Borkh.)

作者:: 刘海莉; 杨蕾蕾; 辛苗苗; 刘晶莹; （西北农林科技大学生命科学学院，陕西省苹果重点实验室，陕西杨凌 712100）

Author(s):: LIU Haili; YANG Leilei; XIN Miaomiao; LIU Jingying; (College of Life Sciences,Northwest A&F University/Shaanxi Key Laboratory of Apple,Yangling,Shaanxi 712100)

关键词:: 苹果; 硅转运蛋白基因; 启动子; 生物信息学

Keywords:: apple; silicon transporter gene; promoter; bioinformatics

DOI:: 10.11937/bfyy.20183232

文献标志码:: A

摘要:: 以“秦冠”苹果叶片为试材，采用RT-PCR技术，克隆了苹果硅转运蛋白基因MdLsi1，并应用生物信息学分析其特征，以期为进一步深入研究硅促进苹果植株生长的分子机制提供参考依据。结果表明：MdLsi1基因全长873 bp，编码290个氨基酸，相对分子量为30.456 kDa。MdLsi1蛋白包含已知硅转运蛋白所具有的特征结构，6个跨膜结构域，2个天冬氨酸-脯氨酸-丙氨酸序列(Asn-Pro-Ala,NPA 模体)，1个由甘氨酸-丝氨酸-甘氨酸-精氨酸（GRGS）组成的ar/R选择性过滤器，并且2个NPA模体之间间隔108个氨基酸。MdLsi1基因起始编码位点前1 500 bp序列中除了包含有基本的基因启动子元件外，还含有与多种逆境胁迫相关的顺式调控元件。以上结果提示MdLsi1在苹果植株硅的积累及植株抵御逆境胁迫中发挥重要功能。

Abstract:: ‘Qinguan’ apple (Malus domestica Borkh.) leaves were used as materials,MdLsi1 gene was cloned by RT-PCR method and characterized using bioinformatic analysis,in order to provide an important basis for the molecular mechanism of silicon as an beneficial element during apple tree growth.The results showed that the full-length ORF of MdLsi1 was 873 bp and encoded 290 amino acid residues,a 30.456 kDa protein.The MdLsi1 showed the characteristic features reported as silicon transporter,which had six transmembrane domains,two conserved NPA motifs,a G-S-G-R ar/R selectivity filter and the 108 aa spacing between NPA motifs.The 1 500 bp upstream region of MdLsi1 was analyzed using PlantCARE databases to identify the presence of various cis-regulatory elements.There were cis-acting elements involved different defense and stress responsiveness besides common cis-acting element in the MdLsi1 promoter.The results suggested that MdLsi1 played an important role in silicon uptake and stress response for apple plant.

参考文献/References:

[1]LIANG Y,NIKOLIC M,BLANGER R,et al.Silicon in Agriculture:From theory to practice[EB/OL].Ed.Springer Netherlands,2015.https://www.springer.com/gp/book/9789401799775.[2]MA J F,TAKAHASHI E.Soil,fertilizer,and plant silicon research in Japan[EB/OL].Elsevier Science,2002.https://www.elsevier.com/books/soil-fertilizer-and-plant-silicon-research-in-japan/ma/978-0-444-51166-9.[3]HODSON M J,WHITE P J,MEAD A,et al.Phylogenetic variation in the silicon composition of plants[J].Annals of Botany,2005,96(6):1027-1046.[4]ALI S,FAROOQ M A,YASMEEN T,et al.The influence of silicon on barley growth,photosynthesis and ultra-structure under chromium stress[J].Ecotoxicolgy and Environmental Safety,2013,89:66-72.[5]FAN X Y,WEN X H,HUANG F,et al.Effects of silicon on morphology,ultrastructure and exudates of rice root under heavy metal stress[J].Acta Physiologiae Plantarum,2016,38(8):197.[6]ETESAMI H,JEONG B R.Silicon (Si):Review and future prospects on the action mechanisms in alleviating biotic and abiotic stresses in plants[J].Ecotoxicolgy and Environmental Safety,2018,147:881-896.[7]MA J F,YAMAJI N.Silicon uptake and accumulation in higher plants[J].Trends in Plant Science,2006,11(8):392-397.[8]MITANI N,YAMAJI N,AGO Y,et al.Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation[J].The Plant Journal,2011,66(2):231-240.[9]DESHMUKH R K,VIVANCOS J,GUERIN V,et al.Identification and functional characterization of silicon transporters in soybean using comparative genomics of major intrinsic proteins in Arabidopsis and rice[J].Plant Molecular Biology,2013,83(4-5):303-315. [10]王慧,赵升,魏珉,等.砧木南瓜硅转运蛋白基因CmLsi3的克隆与表达分析[J].园艺学报,2015,42(10):2075-2082.[11]OUELLETTE S,GOYETTE M H,LABBE C,et al.Silicon transporters and effects of silicon amendments in strawberry under high tunnel and field conditions[J].Frontiers in Plant Science,2017(8):949.[12]邹邦基,何学晖.植物的营养[M].北京:农业出版社,1985.[13]张亚建,武阿锋,刘存寿,等.不同硅肥处理对苹果树硅及其他中微量元素吸收的影响[J].西北农业学报,22(10):126-130.[14]张菂.干旱胁迫及外源施硅对苹果叶片光合机构的影响[D].杨陵:西北农林科技大学,2013.[15]范春丽,赵奇.硅处理对水分胁迫下的苹果幼树生理特征的影响[J].北方园艺,2016(5):30-33.[16]SABER A,LOFT A N,ALI H,et al.Effects of nanosilicon dioxide application on in vitro proliferation of apple rootstock[J].Journal of Plant Nutrition,2016,39(6):850-855.[17]SABER A,LOTFALI N,ALLEN V.Barker Evaluation of nanosilicon dioxide and chitosan on tissue culture of apple under agar-induced osmotic stress[J].Journal of Plant Nutrition,2017,40(20):2797-2907.[18]VATANSEVER R,OZYIGIT I I,FILIZ E,et al.Genome wide exploration of silicon (Si) transporter genes,Lsi1 and Lsi2 in plants;insights into Si-accumulation status/capacity of plants[J].Biometals,2017,30(2):185-200.[19]MA J F,YAMAJI N,MITANI N,et al.An efflux transporter of silicon in rice[J].Nature,2007,448(7150):209-212.[20]YAMAJI N,MITATNI N,MA J F.A transpoter regulating silicon distribution in rice shots[J].Plant Cell,2008,20(5):1381-1389.[21]SARMAST M K.In silco functional analysis of FLC and FT-genes responsible for postponing and acceleration the onset of flowering[J].Journal of Proteomics & Bioinformatics,2017,10(11):267-276.

相似文献/References:

[1]曹慧,姜倩倩,张保仁,等.节水养根处理对苹果枝叶生长发育的影响[J].北方园艺,2013,37(04):1.
[2]王贵平,王金政,薛晓敏,等.叶面喷施甜菜碱对干旱胁迫下苹果幼树[J].北方园艺,2014,38(12):10.
　WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(09):10.
[3]李莹,任亚梅,马学敏,等.1-MCP处理结合不同贮藏条件对苹果常温货架品质的影响[J].北方园艺,2014,38(11):137.
　LI Ying,REN Ya-mei,MA Xue-min,et al.Effects of 1-MCP Treatment With Different Storage Conditions on Quality of Apple During Shelf Life at Room Temperature[J].Northern Horticulture,2014,38(09):137.
[4]党建美,胡清玉,张瑜,等.炭疽菌叶枯病在我国苹果产区的发生分布及趋势分析[J].北方园艺,2014,38(10):177.
　DANG Jian-mei,HU Qing-yu,ZHANG Yu,et al.The Occurrence Trend and Distribution of Apple Glomerella Leaf Spot in China[J].Northern Horticulture,2014,38(09):177.
[5]解贝贝,沙广利,戴洪义,等.六种无融合生殖砧木对‘嘎拉’苹果果实大小和品质的影响[J].北方园艺,2013,37(17):23.
　XIE Bei-bei,SHA Guang-li,DAI Hong-yi,et al.Effect of Six No Fusion Reproductive Rootstocks on the Fruit Size and Quality of ‘Gala’ Apple[J].Northern Horticulture,2013,37(09):23.
[6]冯娟娟,刘军弟,霍学喜.世界主要落叶水果主产国生产与贸易预测分析[J].北方园艺,2014,38(04):195.
[7]吕天星,王铭,伊凯.苹果杂种实生苗叶片性状遗传趋势研究[J].北方园艺,2013,37(14):8.
　LV Tian-xing,WANG Ming,YI Kai.Study on Genetic Trends of Leaf Traits in Apple Hybrid Seedling[J].Northern Horticulture,2013,37(09):8.
[8]史星雲,王立新,王亚杰,等.陕西省苹果主栽品种品质差异及相关性分析[J].北方园艺,2013,37(14):11.
　SHI Xing-yun,WANG Li-xin,WANG Ya-jie,et al.Quality and Correlation Analysis of the Main Apple Cultivars in Shaanxi Province[J].Northern Horticulture,2013,37(09):11.
[9]隗晓雯,郭静,王菲,等.不同矮化砧木叶片酶活性与内源激素含量的差异[J].北方园艺,2013,37(14):16.
　WEI Xiao-wen,GUO Jing,WANG Fei,et al.Difference of Enzyme Activity and the Content of Endogenous Hormones in the Leaves of Different Apple Dwarfing Rootstocks[J].Northern Horticulture,2013,37(09):16.
[10]刘君,刁永强,陈淑英,等.伊犁河谷不同苹果品种苗木冻害调查及分析[J].北方园艺,2013,37(23):34.
　LIU Jun,DIAO Yong-qiang,CHEN Shu-ying,et al.An Investigation and Analysis of Freeze Injury of Different Varieties Apple Seedlings in Ili Area[J].Northern Horticulture,2013,37(09):34.

备注/Memo

第一作者简介：刘海莉（1992-），女，硕士研究生，研究方向为果树逆境分子生物学。E-mail：liuhaili92612@163.com.责任作者：刘晶莹（1982-），女，博士，副教授，现主要从事果树逆境分子生物学等研究工作。E-mail：jingying8233@163.com.基金项目：国家自然科学基金青年资助项目（31401839）。收稿日期：2019-01-15

更新日期/Last Update: 2019-06-03