[1]刘亚,王盼,周钰鸿,等.浙江大盘山映山红菌根真菌的分离和鉴定[J].北方园艺,2019,43(09):77-82.[doi:10.11937/bfyy.20183058]
　LIU Ya,WANG Pan,ZHOU Yuhong,et al.Mycorrhizal Fungi Isolation and Identification of Rhododendron simsii in Dapanshan,Zhejiang[J].Northern Horticulture,2019,43(09):77-82.[doi:10.11937/bfyy.20183058]
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浙江大盘山映山红菌根真菌的分离和鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年09 页码: 77-82 栏目: 出版日期: 2019-05-15
Title:
Mycorrhizal Fungi Isolation and Identification of Rhododendron simsii in Dapanshan,Zhejiang
作者:
刘亚1; 王盼2; 周钰鸿2; 陈江芳3; 陈子林2
（1.浙江省磐安县风景旅游管理局，浙江 磐安 322300；2.浙江省大盘山国家级自然保护区管理局，浙江 磐安 322300；3.磐安县林业局，浙江 磐安 322300）
Author(s):
LIU Ya1; WANG Pan2; ZHOU Yuhong2; CHEN Jiangfang3; CHEN Zilin2
(1.Tourisim Administration Bureau of Pan′an,Pan′an,Zhejiang 322300；2.The Administration Bureau of Dapanshan Natural Reserve,Pan′an,Zhejiang 322300；3.Forestry Bureau of Pan′an,Pan′an,Zhejiang 322300)
关键词:
映山红; 菌根; 菌根真菌; 浙江大盘山
Keywords:
Rhododendron simsii; mycorrhizal; mycorrhizal fungi; Dapanshan; Zhejiang
DOI:
10.11937/bfyy.20183058
文献标志码:
A
摘要:
以浙江大盘山保护区的映山红（Rhododendron simsii）为试材，采用菌落形态分类和rDNA ITS序列分析方法，研究了野生映山红的菌根真菌种类及其组成，以期为栽培杜鹃花的菌根菌剂研发提供菌种参考依据。结果表明：映山红菌根真菌的8个菌种类型均属于子囊菌门，其中5个菌种类型14个菌株都属于Helotiales锤舌菌目，占菌种类型总数的62.5%和菌株总数的66.67%；3个优势菌种类型N5 Phialocephala fortinii、N7 Ilyonectria leucospermi、N8 Nemania sp.分别占菌株总数的42.86%、14.29%和14.29%；此外，还有Rhizodermea veluwensis、Pezicula ericae、Oidiodendron sp.、Helotiales sp.、Uncultured Chaetomium等菌种类型。
Abstract:
Rhododendron simsii in Dapanshan,Zhejiang were taken as experimental material,by means of classification based on colony characteristics and rDNA ITS sequence analysis,mycorrhizal fungi composition of wild Rhododendron simsii was studied,in order to provide references for mycorrhizal fungi inoculant development for cultivated Rhododendrons.The results showed that,all the 8 mycorrhizal fungi species of Rhododendron simsii belonged to Ascomycota,of which 14 strains in 5 mycorrhizal fungi species belonged to Helotiales,accounting for 62.5% of the total species and 66.67% of the total strains;3 dominant mycorrhizal fungi species Phialocephala fortinii,Ilyonectria leucospermi,Nemania sp. respectively accounting for 42.86%,14.29%,14.29% of the total strains;other mycorrhizal fungi species including Rhizodermea veluwensis,Pezicula ericae,Oidiodendron sp.,Helotiales sp.,Uncultured Chaetomium.

参考文献/References:

[1]WU Z Y,RAVEN P H,HONG D Y,et al.Flora of China,Apiaceae through Ericaceae[M]. Volume 14.Beijing: Science Press and Missouri Botanical Garden Press,2005：260-455.[2]熊贤荣,欧阳嘉晖,龙海燕,等.水分胁迫对桃叶杜鹃菌根苗根系超微结构的影响[J].江西农业大学学报,2018,40(5):965-971.[3]刘仁阳,欧静.雷山杜鹃菌根的显微结构与菌根真菌的侵染率[J].贵州农业科学,2014,42(9):109-111.[4]熊贤荣,欧静,龙海燕,等.干旱胁迫对桃叶杜鹃菌根苗生长的影响[J].西南林业大学学报(自然科学版),2018,38(1):34-40.[5]CAIRNEY J W G,MEHARG A A.Ericoid mycorrhiza:A partnership that exploits harsh edaphic conditions[J].European Journal of Soil Science,2010,54(4):735-740.[6]杨秀丽,闫伟.兴安杜鹃菌根真菌分离与鉴定[J].北方农业学报,2016,44(5):54-58.[7]刘仁阳,欧静,李冠楠,等.梵净山雷山杜鹃根部真菌分离与鉴定[J].西北农业学报,2014,23(4):178-185.[8]李丽丽,杨洪一.杜鹃花类菌根真菌研究进展[J].北方园艺,2014(11):173-176.[9]黄彩微,廖映辉,张琪,等.凉山州龙肘山锈红杜鹃与薄叶马银花根部真菌分子检测[J].微生物学通报,2017,44(5):1-18.[10]郑钰,高博,孙立夫,等.银叶杜鹃和繁花杜鹃根部真菌的多样性[J].生物多样性,2010,18(1):76-82.[11]陈真,杨兵,张春英,等.锦绣杜鹃菌根真菌rDNA ITS序列分析及接种效应研究[J].菌物学报,2011,30(5):729-737.[12]陈亚琳,杨碧烟.大盘山志[M].杭州:浙江科学技术出版社,2016:2-8.[13]张春英,尹丽娟,王胤,等.一种简捷的分离杜鹃花类菌根真菌的方法[J].生物技术,2007,17(6):28-32.[14]李敏,姚庆智,魏杰,等.贺兰山紫蘑的分离、鉴定与培养条件[J].北方园艺,2017(14):169-174.[15]龙毅.三种杜鹃属植物菌根真菌的研究[D].贵阳:贵州大学,2008.[16]WALKER J F,ALDRICH-WOLFE L,RIFFEL A,et al.Diverse Helotiales associated with the roots of three species of arctic Ericaceae provide no evidence for host specificity[J].New Phytologist,2011,191(2):515-527.[17]杨秀丽,闫伟.我国杜鹃花科植物菌根及其真菌多样性研究现状[J].安徽农业科学,2015,43(5):7-9.[18]OBASE K,MATSUDA Y.Culturable fungal endophytes in roots of Enkianthus campanulatus (Ericaceae)[J].Mycorrhiza,2014,24(8):635-644.[19]VOHNK M,ALBRECHTOV J,VOSTKA M.The inoculation with Oidiodendron maius and Phialocephala fortinii alters phosphorus and nitrogen uptake,foliar C:N ratio and root biomass distribution in Rhododendron cv.Azurro[J].Symbiosis,2005,40(3):87-96. [20]刘静,刘凤红,宿红艳,等.接种深色有隔内生真菌对蓝莓果实品质的影响[J].北方园艺,2016(20):33-36.[21]AGUSTI-BRISACH,CARLOS,GARCIA-JIMENEZ,et al.Detection of black-foot disease pathogens in the grapevine nursery，propagation process in Spain[J].European Journal of Plant Pathology,2013,137(1):103-112.[22]VITALE A,AIELLO D,GUARNACCIA V,et al.First report of root rot caused by Ilyonectria (=Neonectria) macrodidyma on avocado (Persea americana) in Italy[J].Journal of Phytopathology,2012,160(3):156-159.[23]BRUZONE M C,FONTENLA S B,VOHNK M.Is the prominent ericoid mycorrhizal fungus Rhizoscyphus ericae absent in the southern hemisphere′s Ericaceae? A case study on the diversity of root mycobionts in Gaultheria spp.from northwest Patagonia,Argentina[J].Mycorrhiza,2015,25(1):25-40.[24]张新军.极小种群野生植物大树杜鹃的形态解剖特征及内生真菌多样性研究[D].昆明:云南大学,2015.[25]KO W H.Nature of slow and quick decline of macadamia trees[J].Botanical Studies,2009,50:1-10.[26]何海珊,伍建榕,邱坚,等.花斑木菌种筛选[J].林业科学,2014,50(5):118-122.[27]裔景,程瑾,张启翔.贵州兴义四种兜兰属植物菌根真菌的组成与多样性分析[C].北京：中国观赏园艺研究进展2017,2017.[28]李金霞,曹艳花,白飞荣,等.西沙野生诺尼果内生菌的分离与鉴定[J].食品与发酵工业,2014,40(3):68-75.[29]DENG Z,CAO L.Fungal endophytes and their interactions with plants in phytoremediation:A review[J].Chemosphere,2017,168:1100-1106.[30]YAMAJI K,WATANABE Y,MASUYA H,et al.Root fungal endophytes enhance heavy-metal stress tolerance of Clethra barbinervis growing naturally at mining sites via growth enhancement,promotion of nutrient uptake and decrease of heavy-metal concentrationv[J].PLoS One,2016,11(12):1-15.[31]王艳红,吴晓民,朱艳萍,等.温郁金内生真菌Chaetomium globosum L18对植物病原菌的抑菌谱及拮抗机理[J].生态学报,2012,32(7):2040-2046.[32]刘彩云,季洪亮,王瑞,等.近缘毛壳菌生防菌株LB-1对几种常见植物病原真菌的拮抗作用及其生长适应性分析[J].植物保护学报,2018,45(2):332-339.[33]梁海林,童志武,朱笃.球毛壳菌次级代谢产物及其生物活性研究进展[J].天然产物研究与开发,2018,30(4):609,702-707.

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备注/Memo

第一作者简介：刘亚（1990-），女，硕士，工程师，现主要从事植物生态与植物旅游开发利用等研究工作。E-mail：785480407@qq.com.责任作者：陈子林（1970-），男，硕士，高级工程师，现主要从事林学和药用植物资源保护与开发利用等研究工作。E-mail：dpschzl@163.com.基金项目：金华市重点科技计划资助项目（2017-2-022）。收稿日期：2019-01-19

更新日期/Last Update: 2019-05-31