ZHANG Lipeng,YU Deshui,TENG Yanjiao,et al.Isolation and Investigation of Glutathione S-transferase Genes in Tibet Rhodiola renulata[J].Northern Horticulture,2019,43(06):18-27.[doi:10.11937/bfyy.20183024]
大花红景天谷胱甘肽S-转移酶基因的分离与鉴定
- Title:
- Isolation and Investigation of Glutathione S-transferase Genes in Tibet Rhodiola renulata
- Keywords:
- Rhodiola crenulata; GST; bioinformation; qRT-PCR; expression pattern
- 文献标志码:
- A
- 摘要:
- 以西藏大花红景天为试材，利用生物信息学和实时荧光定量PCR技术对谷胱甘肽S-转移酶（GST）基因家族中的11个成员进行分析，以期为红景天属GST家族基因的功能研究提供参考。结果表明：基于蛋白质序列比对可以将11个大花红景天RcGSTs基因分为6个亚组，分别为tau类（RcGSTU1、RcGSTU2、RcGSTU3）；phi类（RcGSTF1、RcGSTF2、RcGSTF3、RcGSTF4）；lambd类RcGSTL；微粒体类RcGSTM；theta类RcGST1；zeta类RcGSTZ。在线网站分析结果显示仅RcGSTM具有跨膜结构域，并定位于细胞膜，其它RcGSTs均无跨膜结构域并定位于细胞质。组织特异性与胁迫诱导表达模式结果表明，RcGSTs基因具有不同的组织表达特异性；低温胁迫下4个基因表达水平上调，7个基因下调；且脱落酸（ABA）可以诱导RcGSTs基因表达。
- Abstract:
- The glutathione S-transferase (GST) gene family members were analysis by bioinformation and qRT-PCR for Tibet Rhodiola crenulata，in order to lay a foundation for the further studies of function about GST family genes in Rhodiola.The results showed that 11 RcGSTs genes were classified into six subgroups according to protein sequence identity contained tau (RcGSTU1,RcGSTU2,RcGSTU3),phi (RcGSTF1,RcGSTF2,RcGSTF3,RcGSTF4),lambd (RcGSTL),microsomal (RcGSTM),theta (RcGST1),zeta (RcGSTZ).All RcGSTs genes located in cytoplasm and without transmembrane domain expect RcGSTM.The expression patterns for tissue and stresses presented that RcGSTs had different tissue expression specificity.Under cold stress,4 of 11 RcGSTs expression levels were up-regulation and 7 of 11 RcGSTs were down-regulation.For abscisic acid stresses,all RcGSTs were induced the expression levels.
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备注/Memo
第一作者简介：张力鹏（1989-），男，河北沧州人，博士研究生，研究方向为分子细胞遗传学。E-mail:nknanhai@163.com.责任作者：宋文芹（1953-），女，天津人，博士，教授，现主要从事中国主要中药材种质资源与品种改良等研究工作。E-mail:songwq@nankai.edu.cn.收稿日期：2018-10-17