[1]汪洋,于垚,袁宇含,等.膜荚黄芪种子中花青素提取方法的优化[J].北方园艺,2019,43(08):125-130.[doi:10.11937/bfyy.20182917]
　WANG Yang,YU Yao,YUAN Yuhan,et al.Optimization of Extraction Process of Anthocyanin in Seed of Astragalus membranaceus[J].Northern Horticulture,2019,43(08):125-130.[doi:10.11937/bfyy.20182917]
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膜荚黄芪种子中花青素提取方法的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年08 页码: 125-130 栏目: 出版日期: 2019-04-30
Title:
Optimization of Extraction Process of Anthocyanin in Seed of Astragalus membranaceus
作者:
汪洋; 于垚; 袁宇含; 于洋; 全雪丽; 吴松权
（延边大学 农学院，吉林 延吉133002）
Author(s):
WANG Yang; YU Yao; YUAN Yuhan; YU Yang; QUAN Xueli; WU Songquan
(Agriculture College,Yanbian University,Yanji,Jilin 133002)
关键词:
膜荚黄芪; 种子; 花青素; 提取; 优化
Keywords:
Astragalus membranaceus; seed; anthocyanin; extraction; optimization
DOI:
10.11937/bfyy.20182917
文献标志码:
A
摘要:
以膜荚黄芪种子为试材，在单因素试验基础上，采用L25(56)正交实验法，以吸光度为评价指标，优化了花青素提取方法，为膜荚黄芪的开发利用提供参考依据。结果表明：料液比是对花青素提取效果影响最大的因素，最佳提取方法为料液比1∶8 g?mL-1、甲醇体积分数85%、加酸量1.0%、超声时间30 min、超声温度30 ℃，该提取方法简单可靠,具有较高的抗氧化能力。
Abstract:
To provide reference basis for the development and utilization of Astragalus membranaceus,based on one way anova method,L25(56) orthogonal test method was adopted to optimize extraction process of anthocyanin in seeds of A.membranaceus using the absorbance as the evaluation index.The results showed that the main factor for extraction process was the material-liquid ratio,and the optimum material-liquid ratio,methanol volume fraction,acid volume,ultrasonic time,and ultrasonic temperature were 1∶8 g?mL-1,85%,1.0%,30 minutes and 30 ℃,respectively.This extraction method was simple,reliable and had high antioxidant capacity.

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备注/Memo

第一作者简介：汪洋（1992-），男，硕士研究生，研究方向为特种植物资源。E-mail：1761499787@qq.com.责任作者：吴松权（1972-），男，博士，副教授，研究方向为特种植物资源与生物技术。E-mail：arswsq@ybu.edu.cn.基金项目：国家自然科学基金资助项目（21462044）。收稿日期：2018-10-18

更新日期/Last Update: 2019-06-03