LI Xiang,GUO Jinping,ZHANG Yunxiang.Optimization of Factors Affecting Induction and Differentiation of Bud Segments of Eucommia ulmoides Oliver[J].Northern Horticulture,2019,43(05):32-38.[doi:10.11937/bfyy.20182802]
杜仲带芽茎段诱导和分化的组培体系优化
- Title:
- Optimization of Factors Affecting Induction and Differentiation of Bud Segments of Eucommia ulmoides Oliver
- 文献标志码:
- A
- 摘要:
- 以杜仲的幼嫩茎段为试材，采用组织培养的方法，研究了消毒时间、基本培养基、NAA浓度、6-BA浓度、低温处理时间及培养环境对杜仲腋芽诱导及其分化的影响，以期为建立杜仲高效组织培养体系提供依据。结果表明：最佳消毒时间为9 min，污染率与死亡率均较低，分别为10.00%和26.67%；腋芽诱导的基本培养基以MS培养基为最佳，诱导率达91.66%；诱导培养基为MS+0.2 mg?L-1 6-BA+0.03 mg?L-1 NAA时，腋芽的诱导率较高，达93.33%，且生长良好；当低温处理时间为24 h时，外植体诱导效果最好，且褐化率最低，为5.00%；与黑暗培养相比，光照培养条件更加有利于杜仲的腋芽诱导和生长；MS+1.5 mg?L-1 6-BA+0.1 mg?L-1 NAA为愈伤分化的最佳培养基，MS+2.5 mg?L-1 6-BA+0.1 mg?L-1 NAA为芽增殖的最佳培养基。
- Abstract:
- The young stem segments of Eucommia ulmoides Oliver were used as materials，tissue culture method was used，the effects of disinfection time，basic medium，NAA concentration，6-BA concentration，low temperature treatment time and culture environment on the induction of Eucommia ulmoides Oliver axillary buds and the differentiation of Eucommia ulmoides Oliver were investigated，in order to provide a basis for establishing an efficient tissue culture system of Eucommia ulmoides Oliver.The results showed that the best disinfection time was 9 minutes，and the rate of pollution and mortality were lower，10.00% and 26.67% respectively;the basic medium for axillary buds induction was MS medium，with an induction rate of 91.66%;when the induction medium was MS+0.2 mg?L-1 6-BA+0.03 mg?L-1 NAA，the induction rate of axillary buds was 93.33% and it grew well;when the low temperature treatment time was 24 hours，explant induction effect was the best，and browning rate was the lowest，5.00%;compared with dark culture，light culture conditions were more conducive to the induction and growth of axillary buds of Eucommia ulmoides;MS+1.5 mg?L-1 6-BA+0.1 mg?L-1 NAA was the best medium for callus differentiation，MS+2.5 mg?L-1 6-BA+0.1 mg?L-1 NAA was the best medium for bud proliferation.
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备注/Memo
第一作者简介：李响(1993-)，女，硕士研究生，研究方向为森林培育学。E-mail:1061423872@qq.com.责任作者：郭晋平(1963-)，男，博士，教授，博士生导师，现主要从事森林资源培育与经营管理及森林景观生态教学与科研等工作。E-mail：jinpguo@126.com.收稿日期：2018-10-17