XUE Shan,LIU Zeming.Optimization of Extraction Process and Determination of Physicochemical Properties of Water Insoluble Dietary Fiber From Golek Peel[J].Northern Horticulture,2019,43(07):114-121.[doi:10.11937/bfyy.20182665]
鹰嘴芒皮渣水不溶性膳食纤维提取工艺优化及理化性质测定
- Title:
- Optimization of Extraction Process and Determination of Physicochemical Properties of Water Insoluble Dietary Fiber From Golek Peel
- Keywords:
- golek peel; water insoluble dietary fiber; enzymatic method; process optimization; physicochemical properties
- 文献标志码:
- A
- 摘要:
- 以鹰嘴芒皮渣为试材，采用酶法提取鹰嘴芒皮渣中水不溶性膳食纤维（insoluble dietary fiber,IDF），在液料比、糖化酶温度、糖化酶酶解时间、蛋白酶温度、蛋白酶酶解时间和蛋白酶酶解pH 6个单因素试验的基础上，采用Box-Behnken响应面法优化得到了最佳工艺，并对提取的IDF进行理化性质测定，为芒果皮渣的精深加工提供参考。结果表明：液料比、糖化酶作用温度和蛋白酶作用温度3个因素对试验结果影响较为显著。进一步通过响应面试验结果得到IDF的最佳提取工艺为液料比20.5 mL?g-1、糖化酶温度63 ℃、蛋白酶温度51 ℃，此条件下鹰嘴芒IDF的实际得率为28.56%。此外，所提IDF持水力、持油力和膨胀力分别为5.47 g?g-1、1.23 g?g-1和4.69 mL?g-1。IDF在0.1～0.5 mg?mL-1具有良好的?OH清除率（IC50为0.262 mg?mL-1），且呈现出明显的量效关系，是一种优良的抗氧化膳食纤维。
- Abstract:
- Using the golek peel as material,the enzymatic method was used to extract the water insoluble dietary fiber (IDF) from the peel.On the basis of six single factors(liquid-solid ratio,glycosylase temperature,glycosylase digestion time,protease temperature,protease digestion time,protease pH),the optimum process was optimized by box-behnken response surface method,and the physicochemical properties of the IDF were determined,to provide reference for deep-processing for golek peel.The results showed that among the factors,liquid-solid ratio,the temperature of saccharifying enzyme,and the temperature of protease had more significant influence on the experimental results.The best extraction process of IDF by further response surface experiment was as follows,the liquid-solid ratio 20.5 mL?g-1,the temperature of saccharifying enzyme 63 ℃ and temperature of protease 51 ℃.Under these conditions,the actual IDF yield of mango peel was 28.56%.In addition,the water holding capacity of the IDF was 5.47 g?g-1,oil holding capacity was 1.23 g?g-1,expansion force was 4.69 mL?g-1.Besides,IDF had a good ?OH scavenging rate (IC50 was 0.262 mg?mL-1) from 0.1 to 0.5 mg?mL-1,and it had obvious quantitative effect relationship,which is an excellent antioxidant dietary fiber.
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备注/Memo
第一作者简介：薛山（1988-），女，博士，副教授，研究方向为食品科学。E-mail:yixuanchenglion@sina.com.基金项目：闽南师范大学博士科研启动基金资助项目（2006L21513）；2018年福建省中青年教师教育科研资助项目（JT180307）；大闽食品（漳州）有限公司博士后项目基金资助项目；福建省高校杰出青年科研人才培育计划资助项目；供给侧结构改革与新时代区域经济平衡发展研究资助项目(4105/J11815）。收稿日期：2018-11-12