[1]何超,孔琼,袁盛勇,等.阿维菌素对南瓜实蝇的不育作用及谷胱甘肽-S-转移酶活力的影响[J].北方园艺,2019,43(08):40-46.[doi:10.11937/bfyy.20182642]
　HE Chao,KONG Qiong,YUAN Shengyong,et al.Sterile Effects of Avermectin and Its Effect on Activities of Glutathione S-transferase in Bactrocera tau[J].Northern Horticulture,2019,43(08):40-46.[doi:10.11937/bfyy.20182642]
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阿维菌素对南瓜实蝇的不育作用及谷胱甘肽-S-转移酶活力的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年08 页码: 40-46 栏目: 出版日期: 2019-04-30
Title:
Sterile Effects of Avermectin and Its Effect on Activities of Glutathione S-transferase in Bactrocera tau
作者:
何超; 孔琼; 袁盛勇; 沈登荣; 谢昆; 王传铭
（红河学院 生命科学与技术学院，云南省高校农作物优质高效栽培与安全控制重点实验室，云南 蒙自 661100）
Author(s):
HE Chao; KONG Qiong; YUAN Shengyong; SHEN Dengrong; XIE Kun; WANG Chuanming
(College of Life Science and Technology,Honghe University/Crop Cultivation and Safety Control Key Laboratory of Yunnan Province,Mengzi,Yunnan 661100)
关键词:
南瓜实蝇; 阿维菌素; 不育作用; 谷胱甘肽-S-转移酶
Keywords:
Bactrocera tau; abamectin; sterility effect; glutathione S-transferase
DOI:
10.11937/bfyy.20182642
文献标志码:
A
摘要:
以南瓜实蝇为研究对象，采用人工饲料添加法，通过研究阿维菌素不同浓度处理下，南瓜实蝇产卵量、卵孵化率及谷胱甘肽-S-转移酶（GSTs）活性变化，评价阿维菌素对该虫的不育效果，以期为该虫的综合治理提供参考依据。结果表明：阿维菌素对南瓜实蝇具有显著的不育效果。不同浓度处理后，南瓜实蝇单雌产卵量和卵孵化率均显著减少，各浓度不育效果依次为0.10 mg?L-1>0.50 mg?L-1>1.00 mg?L-1>0.05 mg?L-1>0.01 mg?L-1；且单独处理雌成虫不育效果>雌雄成虫均处理>单独处理雄成虫。各浓度处理南瓜实蝇雌雄成虫GSTs活力均随成虫日龄的增加而显著升高，其子代幼虫和蛹的GSTs的活力较对照均表现为不同程度的增加。阿维菌素对南瓜实蝇具有较好的不育作用，且对该虫子代产生了显著影响，为阿维菌素作为南瓜实蝇不育剂应用提供了参考依据。
Abstract:
Bactrocera tau was used as test insect,the artificial infusion method was used to evaluate the infertility effect of avermectin on the adults by studying the fecundity,hatching rate and glutathione S-transferase activity in Bactrocera tau treated with different concentrations of avermectin,in order to provide a theoretical basis for the comprehensive management of the insect.The results showed that avermectin had significant infertility effect on Bactrocera tau.When the concentration of 1.00,0.50,0.10,0.05,0.01 mg?L-1 avermectin and artificial diet were mixed to feed adults of Bactrocera tau,the number of eggs laid per female per day and egg hatching rate was significantly reduced,and the infertility effects of each concentration was as follows:0.10 mg?L-1>0.50 mg?L-1>1.00 mg?L-1>0.05 mg?L-1>0.01 mg?L-1.When different concentrations of avermectin and artificial diet were mixed,the male and female adults were treated separately,the infertility effect was treatment of female adults separately>treatment of both male and female adults>treatment of male adults separately.The activity of GSTs of male and female adults treated with different concentrations of avermectin increased significantly with the increase of adult age,and the activities of GSTs of their larva and pupa progeny showed different degrees of increase compared with the control.It proves that avermectin had infertility to Bactrocera tau,and also has a significant inhibitory effect on the offspring.The results provide a theoretical basis for the application of avermectin as sterility agent for Bactrocera tau.

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备注/Memo

第一作者简介：何超（1979-），男，博士，讲师，现主要从事农业昆虫及害虫综合治理等研究工作。E-mail:hechao1022@163.com.责任作者：袁盛勇（1975-），男，硕士，副教授，现主要从事农业昆虫及害虫综合治理等研究工作。E-mail:ysy9069@163.com.基金项目：国家自然科学基金资助项目(31760638);云南省应用基础研究计划面上资助项目(2016FB069);红河学院植物保护硕士点建设资助项目。收稿日期：2018-10-17

更新日期/Last Update: 2019-05-27