LEI Jiqing,WU Wenneng,LIU Ying,et al.Identification of Pathogen Isolation and Pathogenicity Difference of ‘HongYang’ Kiwifruit Ripe Rot in Liupanshui City,GuiZhou Province[J].Northern Horticulture,2019,43(04):31-38.[doi:10.11937/bfyy.20182522]
贵州六盘水地区“红阳”猕猴桃软腐病病原菌分离鉴定及致病力差异测定
- Title:
- Identification of Pathogen Isolation and Pathogenicity Difference of ‘HongYang’ Kiwifruit Ripe Rot in Liupanshui City,GuiZhou Province
- 文献标志码:
- A
- 摘要:
- 以2016—2017年采集的“红阳”猕猴桃病果为试材,通过病原菌分离、病原菌鉴定及致病力测定,研究了“红阳”猕猴桃软腐病病原菌种类及其致病力差异,以期为“红阳”猕猴桃软腐病防控提供参考依据。结果表明:分离得到的23株病原菌,获得登录号分别为MF498063~498065,MG775300~775302和MG775283~775299;病原菌分属葡萄座腔菌Botryosphaeria sp.、交链孢菌Alternaria sp.、拟茎点霉菌Phomopsis sp.、小新壳梭孢菌Neofusicoccum sp.及间座壳菌Diaporthe sp.,各菌属检出率为30.4%、30.4%、26.1%、8.7%及4.4%;聚类分析将23株病原菌致病力分为弱致病力(26.09%)、中致病力(39.13%)、强致病力(26.09%)和较强致病力(8.69%)。
- Abstract:
- From 2016 to 2017,taking ripe rot kiwifruit collected from planting area in Guizhou Province Liupanshui city as raw material,and then the pathogens and pathogenicity differences were studied by pathogen isolation,pathogen identification,and pathogenicity identification in order to supply references for ripe rot prevention.The results showed that 23 pathogenic bacteria were isolated and obtained accession numbers MF498063-498065,MG775300-775302 and MG775283-775299,respectively;the isolated pathogens were Botryosphaeria sp.,Alternaria sp.,Phomopsis sp.,Neofusicoccum sp.and Diaporthe sp.,the detection rates of respective genus were 30.4%,30.4%,26.1%,8.7% and 4.4%;23 pathogenic bacteria experienced cluster analysis were divided into weak pathogenicity(26.09%),moderate pathogenicity(39.13%),strong pathogenicity(26.09%) and stronger pathogenicity(8.69%).
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备注/Memo
第一作者简介:雷霁卿(1985-),女,硕士,讲师,研究方向为果蔬采后病害。E-mail:2012444896@qq.com.责任作者:王瑞(1978-),男,博士,教授,研究方向为农产品贮运。E-mail:18872762@qq.com.基金项目:省市科技合作资助项目(52020-2016-S-KS-002);贵州省科学技术改革转制资助项目(黔科合体Z字[2015]4001号);国家级创新训练资助项目(201810976017);贵阳市科技局贵阳学院专项资金资助项目(GYU-KYZ[2018]01-05)。收稿日期:2018-09-20