WANG Lei,LIU Chang,YUAN Mengru,et al.SpsLAS2 and SpsLAS3 Gene Cloning,Bioinformatics and Tissue-specific Expression Analysis of Salix psammophila[J].Northern Horticulture,2019,43(10):67-71.[doi:10.11937/bfyy.20182275]
沙柳SpsLAS2和SpsLAS3基因克隆与生物信息学及组织特异性表达分析
- Title:
- SpsLAS2 and SpsLAS3 Gene Cloning,Bioinformatics and Tissue-specific Expression Analysis of Salix psammophila
- Keywords:
- Salix psammophila; SpsLAS2; SpsLAS3; gene cloning; sequence analysis
- 文献标志码:
- A
- 摘要:
- 为促进沙柳(Salix psammophila)的分子定向育种，研究其分枝调控遗传机制，以沙柳为试验材料，采用同源克隆技术克隆获得沙柳SpsLAS2、SpsLAS3基因并做生物信息学及组织特异性表达分析。结果表明：SpsLAS2和SpsLAS3基因序列相似性达96.7%，氨基酸序列相似性达97.6%。SpsLAS2和SpsLAS3基因ORF长分别为1 248、1 254 bp，分别编码415 aa和417 aa。其氨基酸序列具有GRAS(Gibberellin-Insensitive,Repressor of ga1-3,Scarecrow)基因家族典型的5个结构域[1]，属LS亚家族。qRT-PCR组织特异性分析发现2个基因均在叶腋处表达量最高，表明2个基因在叶腋中是特异表达的，可能调节叶腋分生组织的发育形成。SpsLAS2、SpsLAS3相似度较高，但也存在一定差异，推测2个基因可能是同一染色体的串联基因倍增关系。
- Abstract:
- To promote the molecular breeding of Salix psammophila,branching and regulation genetic mechanism was studied. Salix psammophila was used as the experimental material,the SpsLAS2,SpsLAS3 gene of Salix psammophila was cloned by homologous cloning technique and analyzed by bioinformatics and tissue-specific expression.The similarity of SpsLAS2 and SpsLAS3 gene sequences got to 96.69%,and the amino acid sequences similarity got to 97.6%.The sequence lengths of SpsLAS2 and SpsLAS3 were 1 248 bp and 1 254 bp,coding 415 aa and 417 aa,respectively.The amino acid sequences had five typical domains of GRAS gene family and belonged to the LS subfamily.The qRT-PCR in different tissues of Salix psammophila showed that the two genes expressed the highest in the leaf axil,suggesting that the two genes specifically expressed in the leaf axil,and two genes might be involved in the regulation of axillary meristem formation by participating in leaf axillary meristem.It was suggested that the two genes may be tandem gene doubling relationship on the same chromosome.
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备注/Memo
第一作者简介：王雷(1972-)，男，博士，副教授，硕士生导师，研究方向为草坪及园林植物生态学。E-mail:wangzhuoran2003@sina.com.责任作者：杨海峰（1975-），男，博士，副教授，硕士生导师，研究方向为林木基因组学及林木基因工程。E-mail:yang_hf888@163.com.基金项目：国家自然科学基金资助项目(31360187，31660216)；林木遗传育种国家重点实验室(中国林业科学研究院)开放课题资助项目(TGB2015001)；国家科技重大专项资助项目（2018ZX08020002-005-005）。收稿日期：2018-11-23