SU Meifei,WEI Xiaohong,HAN Ting,et al.Effects of Salt-tolerance Exercise on Survival Rate and Physiological Characteristics of Cherry Tomato Seedlings Under Salt Stress[J].Northern Horticulture,2019,43(02):48-54.[doi:10.11937/bfyy.20182123]
杜梨干旱胁迫相关水通道蛋白基因的筛选与克隆
- Title:
- Effects of Salt-tolerance Exercise on Survival Rate and Physiological Characteristics of Cherry Tomato Seedlings Under Salt Stress
- 文献标志码:
- A
- 摘要:
- 以杜梨实生苗为材料，采用15% PEG 6000进行模拟干旱处理，利用qRT-PCR、同源重组、亚细胞定位等方法，研究了不同水通道蛋白基因在15% PEG 6000胁迫条件下表达水平的变化趋势，筛选了响应干旱胁迫相关的水通道蛋白基因，并对其进行克隆和分析，以期为梨抗旱基因筛选提供参考依据。结果表明:AQPs在杜梨的根、茎、叶中均有表达，且随着胁迫时间的延长，大部分AQPs在根系中的表达水平呈先上升后下降的趋势，其中PIP2;2基因在胁迫处理3 h后，相对表达量最高；从杜梨根系中克隆获得PbPIP2;2，该基因包含一个完整的846 bp的开放阅读框，编码281个氨基酸。氨基酸序列比对分析结果表明，PbPIP2;2与其它近缘物种具有一致的保守功能域，其中与苹果MdPIP2;2、白梨PcPIP2;2相似度最高。洋葱表皮亚细胞定位发现PbPIP2;2基因定位在质膜上。
- Abstract:
- For the serious persecution of colonization of cherry tomato seedlings after saline land transplants,‘Delicious’ cherry tomatoes were used as test materials,and the control group was set,salt-resistant exercise group,and non-salt-resistance exercise group were treated.To study the effect of different incremental salt concentrations (NaCl) on the survival rate and physiological indicators of cherry tomato seedlings at different times.The purpose of this study was to provide an effective way and theoretical basis for the break through of the establishment of cherry tomato in the saline-alkali environment with low survival rate and poor growth and development.The results showed that the salt-tolerant exercise group effectively increased the survival rate of cherry tomato seedlings transplanted to saline-alkali soil (33.32%) compared with the non-salt-resistant exercise group;at the same time,salt-tolerant exercise effectively increased the proline content and activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) in cherry tomato seedlings,and reduced malondialdehyde.It indicated that salt-tolerant exercise could improve the osmotic damage of the tomato seedlings by increasing the content of osmotic adjustment substances and antioxidant enzyme activities,and reduce the oxidative damage of cell membranes.In addition,this experiment found that the low salt concentration was better than the high salt concentration range,and the range of 50-150 mmol·L-1 NaCl was the most suitable,and the difference of the concentration gradient was more advanced by 25 mmol·L-1 NaCl.
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备注/Memo
第一作者简介：周菲（1993-），女，硕士研究生，研究方向为梨抗逆机理。E-mail：18363975802@163.com.责任作者：宋健坤（1978-），男，博士，副教授，研究方向为梨品种选育。E-mail：qausjk@126.com.基金项目：山东省联合基金资助项目（ZR2015CL039）；国家现代农业（梨）产业技术体系建设专项资助项目（CARS-29-07）。收稿日期：2018-08-30