[1]韦荣昌,覃芳,唐美琼,等.茉莉酸甲酯对罗汉果SQS、CS和CAS基因表达的影响[J].北方园艺,2019,43(02):42-47.[doi:10.11937/bfyy.20182039]
　WEI Rongchang,QIN Fang,TANG Meiqiong,et al.Effect of Methyl Jasmonate on Expression of SQS,CS and CAS Genes in Siraitia grosvenorii[J].Northern Horticulture,2019,43(02):42-47.[doi:10.11937/bfyy.20182039]
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茉莉酸甲酯对罗汉果SQS、CS和CAS基因表达的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年02 页码: 42-47 栏目: 出版日期: 2019-01-15
Title:
Effect of Methyl Jasmonate on Expression of SQS,CS and CAS Genes in Siraitia grosvenorii
作者:
韦荣昌1; 2; 覃芳1; 唐美琼1; 闫志刚1; 韦莹1; 施力军1
（1.广西药用植物园，广西 南宁 530023；2.广西农业职业技术学院，广西 南宁 530007）
Author(s):
WEI Rongchang1; 2; QIN Fang1; TANG Meiqiong1; YAN Zhigang1; WEI Ying1; SHI Lijun1
(1.Guangxi Botanical Garden of Medicinal Plant,Nanning,Guangxi 530023；2.Guangxi Vocational Technical College of Agriculture,Nanning,Guangxi 530007)
关键词:
罗汉果; 茉莉酸甲酯; 角鲨烯合酶; 葫芦二烯醇合酶; 环阿乔醇合酶
Keywords:
Siraitia grosvenorii; methyl jasmonate; squalene synthase; cucurbitadienol synthase; cycloartenol synthase
DOI:
10.11937/bfyy.20182039
文献标志码:
A
摘要:
以人工授粉后50 d的罗汉果果实为试材，喷施不同浓度的茉莉酸甲酯（Methyl Jasmonate，MeJA），诱导不同时间后取样并提取RNA，采用实时荧光定量PCR测定罗汉果甜苷V代谢途径3个关键酶（角鲨烯合酶：squalene synthase，SQS；葫芦二烯醇合酶：cucurbitadienol synthase，CS；环阿乔醇合酶：cycloartenol synthase，CAS）基因的相对表达量，以期为掌握SQS、CS和CAS的表达规律，进而为提高罗汉果甜苷V的代谢提供参考依据。结果表明：MeJA对SQS和CS的表达均有促进作用，在200 μmol·L-1 的MeJA诱导后12 h和24 h，SQS和CS的相对表达量最高，分别为处理前的11.12倍和9.82倍；MeJA能抑制CAS的表达，在100 μmol·L-1的MeJA处理后6 h，CAS相对表达量降至最低，仅为对照的0.13倍。
Abstract:
The fruits of Siraitia grosvenorii after fertilization for 50 days were used as materials,treated with different concentrations of methyl jasmonate (MeJA).After inducing with MeJA for different times,RNA was extracted from the sample fruits.Expression levels of squalene synthase (SQS),cucurbitadienol synthase (CS) and cycloartenol synthase (CAS) (the genes encoding the key enzymes of mogroside V biosynthetic pathway in S.grosvenorii) were detected by quantitative real-time PCR in order to provide theoretical basis for improving mogroside V metabolism by grasping the rule of SQS,CS and CAS expression.The results showed that the expressions of SQS and CS were increased by the induction of MeJA;12 hours and 24 hours after treatment with 200 μmol·L-1 MeJA,the expression of SQS and CS were the highest,accounting for 11.12 folds and 9.82 folds of that before treatment,respectively.However,the expression of CAS was decreased by the induction of MeJA;6 hours after treatment with 100 μmol·L-1 MeJA,the expression of CAS was the lowest,accounting for only 0.13 folds of that before treatment.

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备注/Memo

第一作者简介：韦荣昌（1983-），男，博士，高级工程师，研究方向为分子生药学。E-mail：wrc830612@163.com.基金项目：国家自然科学基金资助项目（81860678）；广西自然科学基金资助项目（2017GXNSFAA198232，〖JP6〗2015GXNSFBA139149，2015GXNSFBA139134，2016GXNSFAA380174，2017GXNSFAA198280）。收稿日期：2018-08-08

更新日期/Last Update: 2019-02-13