FU Xiuqin,ZHOU Hailin,FANG Zhongming,et al.Study on Tissue Culture and Rapid Propagation System of Cymbidium faberi Rolfe[J].Northern Horticulture,2018,42(24):104-109.[doi:10.11937/bfyy.20181812]
蕙兰组培快繁体系的研究
- Title:
- Study on Tissue Culture and Rapid Propagation System of Cymbidium faberi Rolfe
- Keywords:
- Cymbidium faberi Rolfe; seed germination; rhizome; additives; proliferation; differentiation
- 文献标志码:
- A
- 摘要:
- 以蕙兰种子、根状茎及无菌苗为试材，采用组织培养方法，研究了蕙兰种子萌发、根状茎增殖、根状茎分化阶段适宜的培养基以及蕙兰无菌苗适合的移栽基质等，以期进一步优化蕙兰组培快繁体系。结果表明：蕙兰种子萌发最适培养基为1/2MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+活性炭1 g·L-1，90 d后萌发率为80%；蕙兰根状茎增殖最适培养基为MS+6-BA 3 mg·L-1+NAA 1 mg·L-1+KT 1 mg·L-1+活性炭0.5 g·L-1+赖氨酸0.3 g·L-1，40 d后增殖倍数为2.057；蕙兰根状茎分化出芽最佳培养基为MS+6-BA 8 mg·L-1+NAA 0.5 mg·L-1+柠檬酸钠1.5 g·L-1+山梨醇4.0 g·L-1，40 d后出芽率达到5.125；蕙兰无菌苗种植的最佳基质组合为松栗王∶苔藓=2∶3，60 d成活率达到87.50%。
- Abstract:
- In order to optimize tissue culture and rapid propagation system of Cymbidium faberi Rolfe，the seeds，rhizomes and aseptic seedlings of Cymbidium faberi Rolfe were used as materials to study the appropriate medium in the seed germination，the proliferation of rhizomes and the differentiation stage，and the matrix of seedling transplanting using tissue culture method.The results showed that the optimum medium for the seed germination of Cymbidium faberi Rolfe was 1/2MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+activated carbon 1 g·L-1，the germination rate reached 80% after 90 days，and the suitable medium for the proliferation of Cymbidium faberi Rolfe was MS+6-BA 3 mg·L-1+NAA 1 mg·L-1+KT 1 mg·L-1+activated carbon 0.5 g·L-1+lysine 0.3 g·L-1，and the multiplication rate reached to 2.057 after 40 days；the best medium of bud differentiation from rhizome was MS+6-BA 8 mg·L-1+NAA 0.5 mg·L-1+sodium citrate 1.5 g·L-1+sorbitol 4 g·L-1，and the bud rate was 5.125 after 40 days；the best matrix combination of aseptic seedlings was king chestnut∶moss=2∶3，and the survival rate was 87.50% after 60 days.
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备注/Memo
第一作者简介：付秀芹(1980-)，女，硕士，讲师，研究方向为植物生物技术。E-mail:81700294@qq.com.责任作者：黄玮婷(1986-)，女，硕士，副教授，研究方向为植物生物技术。E-mail:weitingpink@hotmail.com.基金项目：国家自然科学基金资助项目（31301250）；湖北省教育厅科学技术研究资助项目（B2015394）。收稿日期：2018-06-27