TANG Xiwang,TONG Yanan,HE Xin.Reactions of Lettuce Root Exudation to Cadmium Stress[J].Northern Horticulture,2018,42(19):16-22.[doi:10.11937/bfyy.20180755]
生菜根系分泌物对镉胁迫的响应
- Title:
- Reactions of Lettuce Root Exudation to Cadmium Stress
- Keywords:
- cadmium; lettuce; root secretion; soil cadmium availability
- 文献标志码:
- A
- 摘要:
- 为探究生菜根系分泌物对镉胁迫的响应，将4种生菜（“美国大速生”“大橡生二号”“法什菜花叶苦苣”“立生二号”）在含镉（10 mg·L-1）的霍格兰培养液中连续培养3周，每周通过水培法获取根系分泌物溶液。以根系分泌物溶液总有机碳(TOC)含量表征生菜根系分泌能力，以根系分泌物溶液三维荧光光谱扫描结果表征生菜根系分泌物种类，以根系分泌物溶液提取污染土壤镉浓度表征生菜根系分泌物对土壤镉活化能力。结果表明：随着培养时间的增加，4种生菜根系分泌总量在增加，根系分泌物数量和根系分泌物活化土壤镉能力却在降低；相关性分析结果表明，镉胁迫下根系分泌总量与根系分泌物活化土壤镉能力呈极显著负相关(r=-0.59，P<0.01)，与根系分泌物种类数量呈极显著负相关(r=-0.78，P<0.01)；根系分泌物活化镉能力与根系分泌物种类数量呈极显著正相关(r=0.69，P<0.01)。试验表明，在镉胁迫下随着培养时间的增加，生菜根系分泌总量虽然在增加，但根系分泌物种类在减少，对土壤中镉的活化能力在降低。
- Abstract:
- In this study four lettuce varieties (‘American fast growing lettuce’ ‘Daxiangsheng No.2’‘Fashi Cai Huaye Kuju’ and ‘Lisheng No.2’) were cultivated in 10 mg·L-1 Cd contained Hoagland solution for three weeks to real the reactions of root exudation under cadmium stress.The water culture method was used to obtain the root secretion solution at the end of every week.The TOC content (mg·L-1·h-1) of the root secretion solution was used to characterize lettuce roots secretion capacity,the 3-D fluorescence spectrum scan results of the root secretion solution was used to characterize types of lettuce roots secretion,the soil extracted cadmium concentration by the root secretion solution was used to characterize the capacity of lettuce roots secretion to activate soil cadmium.The results showed that the roots secretion capacity was increased by incubation time,while the number of root secretion and cadmium activation capacity of root secretion showed a trend of decrease.The root secretion capacity was both significant negative related to the root secretion activate ability to soil cadmium (r=-0.59，P<0.01),and types of root secretion (r=-0.78，P<0.01),while the root secretion activate ability to soil cadmium was significant positive related to the types of root secretion (r=0.69，P<0.01).That meant while under cadmium stress,the lettuce roots secretion capacity would increase while the types of root secretion would decrease,that would result in the reducing of Cd activation ability of the lettuce root secretion.
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备注/Memo
第一作者简介：唐希望（1991-），男，博士，讲师，研究方向为设施蔬菜土壤重金属污染与阻控。E-mail：tangxiwang@outlook.com.责任作者：何鑫（1980-），男，硕士，副教授，现主要从事环境微生物等研究工作。E-mail：hex80@163.com.基金项目：河北省重点研发计划资助项目（18222906D）；河北省教育厅高等学校科学技术研究资助项目（QN2018141）；公益性行业(农业)科研专项资助项目(201203045)。收稿日期：2018-04-12