LI Chenghua,XUE Changsong,LI Li.Optimized Extraction and Antioxidant Activity of Flavonoid From Physali alkekengi L.[J].Northern Horticulture,2018,42(23):124-131.[doi:10.11937/bfyy.20180722]
酸浆宿萼总黄酮提取工艺优化及其抗氧化活性
- Title:
- Optimized Extraction and Antioxidant Activity of Flavonoid From Physali alkekengi L.
- Keywords:
- the calyx of Physali alkekengi L.; flavonoids; process optimization; different part; antioxidant capacity
- 文献标志码:
- A
- 摘要:
- 以酸浆宿萼为试材，研究了料液比、乙醇浓度、提取温度、提取时间对总黄酮得率的影响，在单因素试验的基础上，采用四因素三水平的响应面优化工艺条件，通过DPPH法、ABTS+法及FRAP法评价提取物黄酮体外抗氧化能力,为进一步开发酸浆各入药部位活性成分提供参考。结果表明：最佳提取工艺为料液比1∶25 g·mL-1，乙醇浓度54%，提取温度61 ℃，提取时间1.8 h；在此条件下，酸浆宿萼总黄酮提取量为10.13±0.11 mg·g-1，酸浆叶总黄酮提取量为11.08 mg·g-1，酸浆茎总黄酮提取量为5.74 mg·g-1，酸浆果实总黄酮提取量为1.63 mg·g-1；酸浆不同入药部位总黄酮抗氧化能力不同，差异显著(P<0.05)；酸浆茎总黄酮对DPPH·清除能力最强(P<0.05)，酸浆果实、茎总黄酮对ABTS+·清除活性比较强(P<0.05)，FRAP法测得酸浆宿萼、茎、叶总黄酮抗氧化活性能力比较强(P<0.05)。
- Abstract:
- Taking Physali alkekengi L.as the raw material,the effects of extraction conditions such as solid ratio,ethano,extraction temperature,time were investigated by one-factor and the response surface method，and the extraction flavonoids analyzed with DPPH·,ABTS+·,FRAP test.The results showed that the optimum conditions for flavonoids extraction were determined as follows:solid-liquid ratio 1∶25 g·mL-1,ethanol concentration 54%,extraction temperature 61 ℃,extraction time 1.8 hours;under these conditions,the extraction yield of flavonoids from the calyx of Physali alkekengi L.was 10.13±0.11 mg·g-1,the extraction yield of flavonoids from the leaf of Physali alkekengi L.was 11.08 mg·g-1,the extraction yield of flavonoids from the stems of Physali alkekengi L.was 5.74 mg·g-1,the extraction yield of flavonoids from the fruit of Physali alkekengi L.was 1.63 mg·g-1.The antioxidant capacity of the total flavonoids in different part was different(P<0.05).The flavonoids of the stems was the most powerful for DPPH·(P<0.05).The flavonoids of the fruit and stems was stronger for ABTS+·(P<0.05).The tioxidant activity of the calyx,stem and leaves was stronger in FRAP method(P<0.05).
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备注/Memo
第一作者简介：李成华（1978-），女，硕士，副教授，现主要从事古方的现代应用等研究工作。E-mail:105086198@qq.com.基金项目：吉林省教育科学“十三五”规划资助项目（GH170568）；通化师范学院高等教育教学改革研究资助项目（JY2017029）。收稿日期：2018-04-27