[1]陈阳,孙华山,金一锋,等.不同激素对四种草地早熟禾种子愈伤组织的诱导[J].北方园艺,2018,42(21):66-71.[doi:10.11937/bfyy.20180627]
　CHEN Yang,SUN Huashan,JIN Yifeng,et al.Induction of Seed Callus From Four Kinds of Kentucky Bluegrass Seeds With Different Hormones[J].Northern Horticulture,2018,42(21):66-71.[doi:10.11937/bfyy.20180627]
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不同激素对四种草地早熟禾种子愈伤组织的诱导

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年21 页码: 66-71 栏目: 出版日期: 2018-11-10
Title:
Induction of Seed Callus From Four Kinds of Kentucky Bluegrass Seeds With Different Hormones
作者:
陈阳1; 2; 孙华山1; 金一锋2; 王佳贺2; 陈雅君1
（1.东北农业大学 园艺园林学院，黑龙江 哈尔滨 150030；2.齐齐哈尔大学 生命科学与农林学院,黑龙江 齐齐哈尔 161006）
Author(s):
CHEN Yang1; 2; SUN Huashan1; JIN Yifeng2; WANG Jiahe2; CHEN Yajun1
(1.College of Horticulture and Garden,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.College of Life Science and Agriculture,Qiqihar University,Qiqihar,Heilongjiang 161006)
关键词:
草坪草; 草地早熟禾; 组织培养; 愈伤组织
Keywords:
turf grass; Kentucky bluegrass; tissue culture; callus
DOI:
10.11937/bfyy.20180627
文献标志码:
A
摘要:
以4种草地早熟禾（Poa pratensis L.）为试材，以成熟种子为外植体，筛选了适宜的消毒方法及各品种适宜的愈伤组织诱导培养方式，为后续建立高效便捷的草地早熟禾再生体系提供依据。结果表明：最佳的消毒方式为1% NaClO消毒7 min，蒸馏水清洗4～5遍，再用无菌水浸种12 h，接种前用75%乙醇消毒40 s，用无菌水清洗4～5遍，1% NaClO 消毒10 min后接种；4种草地早熟禾基因型不同，其诱导愈伤组织的出愈率有显著差异（P<0.05）；“午夜2号”早熟禾种子诱导最适宜培养基为MS+1 mg·L-1 2,4-D+0.1 mg·L-1 6-BA，“公园”与“黑虎”早熟禾种子诱导最适宜培养基为MS+6 mg·L-1 2,4-D，“雪狼”早熟禾种子诱导最适宜培养基为MS+1 mg·L-1 2,4-D。
Abstract:
In this study,four Kentucky bluegrass varieties were used as materials for the screening of appropriate sterilization and callus induction methods with the mature seeds as explants,in order to supply reference for bluegrass use.The results showed that,the optimal sterilization method was to treat the seeds by 1% NaClO for 7 minutes followed by 4-5 times of washing by distilled water.Subsequently,the seeds were immersed with sterile water for 12 hours.Right before inoculation,the seeds were again washed with 75% ethanol for 40 seconds and then with sterile water for 4-5 times,followed by 10 minutes sterilization by 1% NaClO.The four Kentucky bluegrass varieties possessed different genotypes as well as significantly different frequencies of callus induction (P<0.05).The optimal culture medium was MS+1 mg·L-1 2,4-D+0.1 mg·L-1 6-BA for Kentucky bluegrass ‘Midnight II’,MS+6 mg·L-1 2,4-D for Kentucky bluegrass ‘Park’ and ‘Black Tiger’,and MS+1 mg·L-1 2,4-D for Kentucky bluegrass ‘Snow Wolf’,respectively.

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备注/Memo

第一作者简介：陈阳（1986-），女，黑龙江齐齐哈尔人，博士研究生，研究方向为园林植物抗逆分子生物学。E-mail:chenyang8368215@126.com.责任作者：陈雅君（1964-），女，博士，教授，研究方向为园林植物抗逆分子生物学。E-mail:chenyajun622@163.com.基金项目：国家自然科学基金资助项目（31501785）；黑龙江省自然科学基金资助项目（QC2017026）；黑龙江省教育厅基本业务专项资助项目（135209314）。收稿日期：2018-04-16

更新日期/Last Update: 2018-12-19