JIANG Cheng,WANG Jingjia,LI Jiamei,et al.Effect of Cr6+ on the Seed Germination and Chlorophyll Content of Viola tricolor[J].Northern Horticulture,2018,42(19):78-82.[doi:10.11937/bfyy.20180292]
重金属铬(Cr6+)对三色堇种子萌发及叶绿素含量的影响
- Title:
- Effect of Cr6+ on the Seed Germination and Chlorophyll Content of Viola tricolor
- Keywords:
- Cr; Viola tricolor; seed germination; seedling growth; chlorophyll
- 文献标志码:
- A
- 摘要:
- 以三色堇种子为试材,采用水培法,研究了不同浓度的Cr6+胁迫对三色堇种子萌发及幼苗生长的影响,以期明确重金属铬对三色堇幼苗的危害及作用机制,为后续研究提供一定的参考依据。结果表明:发芽势和发芽率均在0~5.0 mg·L-1 Cr6+处理浓度范围内呈上升趋势,在5.0~80.0 mg·L-1范围内呈下降趋势;在0~80.0 mg·L-1 Cr6+处理浓度〖JP+2〗范围内,随着Cr6+浓度的升高,三色堇幼根长度显著降低,幼芽长度先上升后下降,在2.5 mg·L-1时达到峰值;在0~5.0 mg·L-1 Cr6+处理浓度范围内能够使三色堇子叶中叶绿素a、叶绿素b和叶绿素总量上升,在5.0~40.0 mg·L-1范围内叶绿素a、叶绿素b和叶绿素总量下降。
- Abstract:
- The Viola tricolor seed was used as materials and water culture was used to explore the effects of different concentrations of hexavalent chromium ion stress on seed germination and seedling growth of Viola tricolor.The purpose of this study was to clear the harm and mechanism of chromium on Viola tricolor,and provide the basis for further research.The results showed that germination energy and germination rate were increased when the concentration of Cr6+ range was 0-5.0 mg·L-1,and decreased when the concentration of Cr6+ range was 5.0-80.0 mg·L-1;when the concentration of Cr6+ range was 0-80.0 mg·L-1,with the increasing of the concentration of Cr6+,root length of Viola tricolor decreased significantly,the seedling length first increased and then decreased,and there was the maximum when the the concentration of Cr6+ was 2.5 mg·L-1.When the concentration of Cr6+ range was 0-5.0 mg·L-1,the concentration of chlorophyll a,chlorophyll b and total chlorophyll were increased,and that was decreased when the concentration of Cr6+ range was 5.0-40.0 mg·L-1.
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备注/Memo
第一作者简介:姜成(1978-),男,硕士,讲师,研究方向为重金属污染生物修复及植物生理生化。E-mail:jiang780920@163.com.责任作者:吴恒梅(1972-),女,硕士,副教授,现主要从事植物生理与分子生物学等研究工作。E-mail:hengmeiwu@163.com.基金项目:佳木斯大学科学技术研究资助项目(S2014-014);黑龙江省大学生创新创业计划资助项目(2014xj04)。收稿日期:2018-03-22