[1]付文婷,蓬桂华,罗泽虎,等.一种贵州地方线椒根尖预处理及核型分析[J].北方园艺,2018,42(18):52-56.[doi:10.11937/bfyy.20180237]
　FU Wenting,PENG Guihua,LUO Zehu,et al.Root Tip Pretreatment and Karyotype Analysis of a Local Line Pepper in Guizhou[J].Northern Horticulture,2018,42(18):52-56.[doi:10.11937/bfyy.20180237]
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一种贵州地方线椒根尖预处理及核型分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年18 页码: 52-56 栏目: 出版日期: 2018-09-25
Title:
Root Tip Pretreatment and Karyotype Analysis of a Local Line Pepper in Guizhou
作者:
付文婷; 蓬桂华; 罗泽虎; 廖芳芳; 胡明文
（贵州省农业科学院 辣椒研究所，贵州 贵阳 550006）
Author(s):
FU Wenting; PENG Guihua; LUO Zehu; LIAO Fangfang; HU Mingwen
(Pepper Institute,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006)
关键词:
线椒; 根尖; 预处理; 核型分析
Keywords:
line pepper; root tip; pretreatment; karyotype analysis
DOI:
10.11937/bfyy.20180237
文献标志码:
A
摘要:
以一种地方线椒萌发种子根尖为材料，对影响染色体制片效果的取样时间、预处理试剂及时间进行了研究，并对其进行核型分析，以期优化辣椒染色体制片技术，了解其核型特征，为今后研究不同类型辣椒的亲缘关系和系统进化提供科学基础和细胞学依据。结果表明:10:00取根尖，分裂细胞和中期分裂相细胞最多；经过0.002 mol·L-1 8-羟基喹啉预处理3 h,可获得分散良好、形态最佳、易于进行计数和核型分析的高质量染色体制片；辣椒的核型公式为2n=2x=24=22m+2sm，核型不对称系数为54.0%，属于2A核型。该方法明确了地方线椒染色体压片的最佳预处理参数，并从细胞遗传学角度揭示了地方线椒的核型特征。
Abstract:
A local line pepper root tip was used as plant material.Factors that affected chromosome preparation including sampling time，pretreatment reagents and time were analyzed,in order to optimize the chromosome preparation procedure and investigate the karyotype of line pepper and to provide a theoretical basis for studying genetic and phylogenetic relationships of different types of malabar spinach．The results showed that the percentage of the mitotic cells and metaphase cells reached maximum when sampled at 10:00;the best contraction,shape and dispersion of chromosomes were found at 3 hours 8-hydroxyquinoline pretreatment,which were easy for counting and karyotype.The karyotype formual was 2n=2x=24=22m+2sm，karyotype asymmetry belongs to 2A and the karyotype asymmetry index was 54.0%.This study established an optimal chromosome pretreatment for a local line pepper in Guizhou，and revealed its karyotypic characteristics from the cytogenetic aspects．

相似文献/References:

[1]赵 庆,唐道城.万寿菊根尖染色体观测方法的优化[J].北方园艺,2013,37(20):98.
　ZHAO Qing,TANG Dao-cheng.Optimization of Microscope Observation Method on Root-tip Chromosomes of Marigold[J].Northern Horticulture,2013,37(18):98.

备注/Memo

第一作者简介：付文婷（1984-），女，硕士，副研究员，现主要从事辣椒种质遗传改良和创新等研究工作。E-mail：fwt2010@126.com.基金项目：贵州省科技计划资助项目（黔科合NY[2015]3018-1号,黔科合NY[2014]3040号）；黔农科院院专项资助项目（[2015]24号）。收稿日期：2018-03-19

更新日期/Last Update: 2018-10-17