LI Lei,YAN Min,ZHAO Jia,et al.Analysis of Orchard Branches Composting on Soil Bacterial Diversity[J].Northern Horticulture,2018,42(21):43-48.[doi:10.11937/bfyy.20180049]
果园枝条堆肥细菌群落多样性分析
- Title:
- Analysis of Orchard Branches Composting on Soil Bacterial Diversity
- Keywords:
- compost; fermentation; bacterial community; high throughput sequencing
- 文献标志码:
- A
- 摘要:
- 以果园枝条、新鲜羊粪为试材，采用添加外源发酵剂的方法进行堆肥处理，利用第二代高通量测序技术研究了堆肥过程中细菌群落的变化情况，以期为果园枝条废弃物的资源化循环利用提供参考依据。结果表明：12个样本共得到722 931条高质量序列，测序深度可以真实体现所取样本中的细菌群落多样性。堆肥不同时段的细菌群落结构差异显著，发酵剂细菌群落多样性最小，所含种属最少；添加发酵剂后可显著改变堆肥的细菌群落多样性，发酵过程中芽孢杆菌和放线菌成为优势菌群，为果枝有机肥在后续施用过程中发挥促生作用奠定了基础。
- Abstract:
- To provide the basis for orchard branches waste resource recycling,the branches and fresh manure were used as test materials.The second generation of high-throughput sequencing technologies was used to analysis the bacterial community variation of the compost that adding exogenous starter.The results showed that total 722 931 high quality sequences were got from 12 samples.Sequencing depth could really reflect the sampling of the bacterial community diversity.Significant difference was found in bacterial community structure at different times of the composting and putrefaction.Base on the diversity index and heat map of genera analysis,fermenting agents had minimum bacteria community diversity and genera.The compost′s bacterial community was changed because of the fermenting agents,the Bacillus and Actinomycetes had become advantage groups during the composting and putrefaction,this would promote the growth of plant in future.
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备注/Memo
第一作者简介：李磊(1973-)，男，本科，副研究员，现主要从事植物营养学与土壤改良等研究工作。E-mail:tfslilei@163.com.责任作者：闫敏（1975-），女，硕士，副研究员，现主要从事分子生物学及微生物与植物营养等研究工作。E-mail:ymrice@163.com.基金项目：国家重点研发计划资助项目（2016YFD0201133）；〖JP〗山西“农谷”研发专项资助项目（YCX2017D2209）。收稿日期：2018-02-24