SHI Jianhua,QI Lianfen,WANG Dandan,et al.Effect of Different Concentrations of 〖WT4BZ〗10%〖WT4HZ〗 Berberine on Tomato Seedlings[J].Northern Horticulture,2018,42(16):7-12.[doi:10.11937/bfyy.20174831]
不同浓度10%小檗碱对番茄幼苗的影响
- Title:
- Effect of Different Concentrations of 〖WT4BZ〗10%〖WT4HZ〗 Berberine on Tomato Seedlings
- Keywords:
- tomato seedlings; 10% berberine; chlorophyll; enzyme activity
- 文献标志码:
- A
- 摘要:
- 以“阿里山”番茄为试验材料，采用穴盘育苗方式，设置0（CK）、8、16、32、64、128 mg·L-1不同浓度10%小檗碱，分别测定番茄出苗率、生理指标、相关酶活性，确定番茄幼苗生长的最适宜10%小檗碱（BBR）可湿性粉剂浓度。结果表明：32 mg·L-1和64 mg·L-1处理的番茄幼苗出苗最快，出苗率最高；32 mg·L-1处理下番茄叶绿素含量和过氧化物酶活性均最高，分别为0.76 mg·g-1、4 802.89 U·g-1 ·min-1FW，比CK分别提高16.43%、47.45%。32 mg·L-1处理的番茄幼苗丙酮酸激酶和α-淀粉酶活性均最低，分别为505.18 U·min-1，6.22 mg·g-1·min-1，比CK分别降低10.77%、7.95%。表明番茄幼苗生长最佳的10%小檗碱浓度为32 mg·L-1。
- Abstract:
- Tomato variety of ‘Alishan’ was used as the experimental material,six different concentrations of 10% berberine (0,8,16,32,64,128 mg·L-1) were set,to study the effect of different concentrations of 10% berberine (BBR) WP on germination rate and the physiological index of tomato enzyme activity of tomato seedlings by plug seedling raising method,and to determine the optimum concentration of 10% berberine for the growth of tomato seedlings.The results showed that,tomato seedlings treated with 32 mg·L-1 and 64 mg·L-1 had the highest emergence rate and the highest emergence rate.32 mg·L-1 10% berberine treatment of tomato seedlings had the highest chlorophyll content and peroxidase activity,which were 0.76 mg·g-1 and 4 802.89 U·g-1 ·min-1FW,increased 16.43%,47.45%,respectively.The pyruvate kinase and α-amylase activities of tomato seedlings treated with 32 mg·L-1 were the lowest,which were 505.18 U·min-1 and 6.22 mg·g-1·min-1,respectively,and decreased 10.77% and 7.95% compared to the control treatment,respectively.It showed that the best 10% berberine concentration in tomato seedlings was 32 mg·L-1.
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备注/Memo
第一作者简介：师建华（1966-），女，河北石家庄人，本科，高级农艺师，现主要从事设施蔬菜栽培和工厂化育苗等研究工作。E-mail:13785101151@163.com.责任作者：田国英（1964-），男，河北石家庄人，本科，研究员，现主要从事植物保护等研究工作。E-mail:tguoying1@163.com.基金项目：河北省科技计划资助项目（17226915D）；石家庄市科学技术研究与发展计划资助项目（161490132A）。收稿日期：2018-04-12