ZHANG Xuanzhe,GUO Yanjin,LI Lu,et al.Analysis of Ultrastructural Changes of Cucumber Powdery Mildew Under SOL Biological Agent Treatment[J].Northern Horticulture,2018,42(17):19-26.[doi:10.11937/bfyy.20173602]
SOL生物制剂对黄瓜白粉病的超显微结构变化分析
- Title:
- Analysis of Ultrastructural Changes of Cucumber Powdery Mildew Under SOL Biological Agent Treatment
- Keywords:
- cucumber powdery mildew; soybean oil and lecithin; biological characteristics; control mechanism; ultrastructure
- 文献标志码:
- A
- 摘要:
- 以SOL生物制剂作为试验药剂，采用曲利苯兰染色法和扫描电镜技术，研究了SOL生物制剂对黄瓜白粉病菌的抑菌作用，以期为生物制剂对黄瓜白粉病抑菌机理研究提供参考依据。结果表明：采用曲利苯兰染色法对SOL药剂预防效果进行显微层面初探，观察发现经SOL处理后白粉菌分生孢子与阳性对照甲基托布津及清水处理相比产生芽管时间明显延长，具有预防作用。扫描电镜技术表明了SOL对白粉菌有抑菌作用，SOL作为保护性药剂时，白粉菌分生孢子出现干瘪、皱缩，芽管生长受抑制，无法继续形成侵入钉侵染寄主植物；SOL作为治疗性药剂时，已形成的芽管难以继续生长，白粉菌分生孢子出现干瘪、坍塌，芽管出现断裂、内含物外泄。
- Abstract:
- SOL biological agent was used as test agent.The antimicrobial mechanism of SOL biological agents to powdery mildew of cucumber was observed by triphenyl blue staining and scanning electron microscope,in order to supply references for antimicrobial mechanism of biological agent against powdery mildew.The results showed that the formation time of germ tube of S.fuliginea treated with SOL was significantly prolonged compared with that of positive control thiophanate and clear water treatments,and had preventive effect.With SOL as protective agent,conidia spores of S.fuliginea appeared dry,shrink,and the growth of the germ tube was inhibited.Because S.fuliginea did not continue to form infection tube,S.fuliginea strain was unable to infect host plants.With SOL as a therapeutic agent,it was found that the formed germ tube was difficult to continue to grow,conidia spores of S.fuliginea appeared dry,shrink,the resulting germ tube appeared broken and inclusions leakage.
相似文献/References:
[1]范瑛阁,赵伟,何毅.黄瓜白粉病生防菌的筛选及广谱性测定[J].北方园艺,2013,37(12):116.
FAN Ying-ge,ZHAO Wei,HE Yi.Screening of Bio-control Agents and Broad Spectrum Measurement on Cucumber Powdery Mildew[J].Northern Horticulture,2013,37(17):116.
[2]张丽萍,咸文荣.8%氟硅唑微乳剂对黄瓜白粉病的防效试验[J].北方园艺,2012,36(04):142.
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[3]郭世保,陈俊华,史洪中,等.拿敌稳防治黄瓜白粉病应用研究[J].北方园艺,2013,37(16):137.
GUOShi-bao,CHENJun-hua,SHIHong-zhong,et al.StudyonApplicationofNativoinControllingtoCucumberPowderyMildew[J].Northern Horticulture,2013,37(17):137.
[4]范 瑛 阁,龚 明 福,朱 丽 霞.黄瓜白粉病菌生防菌筛选及生防机制初步研究[J].北方园艺,2010,34(14):0.[doi:10.11937/bfyy.2010014054]
FAN Ying-ge,GONG Ming -fu,ZHU Li -xia.Screening and Preliminary Study of Biocontrol Mechanism on Biocontrol Agents Against Cucumber Powdery Mildew[J].Northern Horticulture,2010,34(17):0.[doi:10.11937/bfyy.2010014054]
备注/Memo
第一作者简介：张铉哲（1970-），男，博士，教授，硕士生导师，研究方向为植物病害综合防治。E-mail:zhe3850@163.com.基金项目：黑龙江省自然科学基金资助项目(C2016019)；黑龙江省经济作物现代农业产业技术协同创新体系资助项目。收稿日期：2018-04-18