WANGYu,HUANGLiang,PENGMingmeng,et al.OptimalLiquidFermentationConditionsofWhiteFlammulinavelutipes[J].Northern Horticulture,2018,42(20):148-153.[doi:10.11937/bfyy.20173564]
白色金针菇液体发酵培养条件的优化
- Title:
- OptimalLiquidFermentationConditionsofWhiteFlammulinavelutipes
- 文献标志码:
- A
- 摘要:
- 以白色金针菇为试材，以菌丝体生物量、密度、萌发时间等为考察指标，采用单因素试验及L9（34）正交实验对影响其液体摇瓶培养时的条件因素（培养基的初始pH、接种龄、接种量、摇床转速）进行优化，以实现白色金针菇摇瓶液体菌种高密度发酵。结果表明：适宜的摇瓶培养条件为培养基初始pH6.5，接种龄102h,接种量10%，摇床转速140r·min-1。在上述条件下，金针菇菌丝体生物量达到17.93g·L-1，密度104.21个·mL-1，直径1.35mm，萌发时间8h。
- Abstract:
- WhiteFlammulinavelutipeswasusedasmateril,theoptimalcultureconditions(mediuminitialpH,inoculumage,inoculumamount,shakerspeed)werestudiedbysinglefactortestandL9(34)orthogonaltest,indexesformycelialbiomass,density,germinationtime,inordertoachievethehighdensityfermentationofwhiteFlammulinavelutipes.TheresultsshowedthattheoptimalcultureconditionsweretheinitialpH6.5,inoculumage102hours,inoculumamount10%,theshakingspeed140r·min-1.Undertheconditions,themycelialbiomassofFlammulinavelutipesreached17.93g·L-1,densitywas104.21·mL-1,thediameterwas1.35mm,thegerminationtimewas8hours.
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备注/Memo
第一作者简介：王玉（1980-），女，博士，副教授，现主要从事食用菌液体发酵等研究工作。E-mail：wytjac@163.com.责任作者：班立桐（1972-），男，本科，教授，现主要从事食用菌栽培等研究工作。E-mail：banlitong@126.com.基金项目：天津市农业科技成果转化与推广资助项目(201303010)；天津市蔬菜产业技术体系创新团队资助项目（ITTVRS2017015）。收稿日期：2018-05-08