XIA Bolin,XING Yide,GAO Fei,et al.Expression Analysis and Construction of Plant Expression Vector of AmWRKY53 in Ammopiptanthus mongolicus[J].Northern Horticulture,2018,42(06):1-9.[doi:10.11937/bfyy.20173341]
蒙古沙冬青AmWRKY53基因表达分析及表达载体构建
- Title:
- Expression Analysis and Construction of Plant Expression Vector of AmWRKY53 in Ammopiptanthus mongolicus
- Keywords:
- Ammopiptanthus mongolicus; AmWRKY53; expression patterns; construction of plant expression vector
- 文献标志码:
- A
- 摘要:
- 以蒙古沙冬青为试材，分离到1个编码WRKY类转录因子基因，命名为AmWRKY53。该序列包含1个长为1 065 bp的开放阅读框，编码354个氨基酸，具有1个WRKYGQK结构域和1个C2HC锌指基序，属于Ⅲ型WRKY转录因子。荧光定量PCR方法检测了非生物胁迫下AmWRKY53在叶和根组织中的表达特点。结果表明：AmWRKY53受干旱和高盐诱导，参与逆境胁迫应答。构建的pPZP212-AmWRKY53植物表达载体，为进一步研究AmWRKY53的功能奠定了基础。
- Abstract:
- In this study,a gene encoding WRKY transcription factor was cloned from Ammopiptanthus mongolicus,and it was named as AmWRKY53.The sequence contained an open reading frame (ORF) of 1 065 bp,which encoded peptide with 354 amino acids.There were one WRKYGQK domain and one C2HC zinc finger motif in AmWRKY53〖STBZ〗,so it belonged to type Ⅲ WRKY transcription factor.Quantitative real-time PCR (qRT-PCR) analyses revealed that the AmWRKY53 was induced by drought and high salt stress.The pPZP212-[STBX]AmWRKY53 expression vector was constructed and laid the foundation for further study of AmWRKY53.
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相似文献/References:
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备注/Memo
第一作者简介：夏波林（1990-），男，湖北来凤人，硕士研究生，研究方向为植物抗逆分子生物学。E-mail：xiabolin@muc.edu.cn.责任作者：周宜君（1964-），女，福建福州人，博士，教授，现主要从事植物抗逆分子生物学等研究工作。E-mail：zhouyijun@muc.edu.cn.基金项目：国家自然科学基金资助项目(31370356,31670335)；中央民族大学一流大学一流学科资助项目(YLDX01013);中央民族大学本科生资助项目（SHSY2016120009）。收稿日期：2017-11-21