W Siyuan,W Genkun,WANG Pengyue,et al.Effcet of Different EMS Concentrations on Seed Germination and Seedling Growth of Chinese Kale[J].Northern Horticulture,2018,42(21):72-75.[doi:10.11937/bfyy.20172867]
不同甲基磺酸乙酯（EMS）浓度对芥蓝种子萌发及种苗生长的影响
- Title:
- Effcet of Different EMS Concentrations on Seed Germination and Seedling Growth of Chinese Kale
- Keywords:
- Chinese kale; EMS; germination rate; seedling survival rate; conductivity
- 文献标志码:
- A
- 摘要:
- 以“中花”芥蓝（“中花”）、“银绿”芥蓝（“银绿”）和矮脚香菇（“25号”）3个芥蓝品种为试材，采用甲基磺酸乙酯（EMS）处理芥蓝种子，研究了不同浓度EMS处理对芥蓝种子萌发和幼苗生理指标的影响，以期筛选出诱变芥蓝种子最合适EMS浓度。结果表明：随着EMS浓度的升高，芥蓝种子的发芽率和成苗率降低，幼苗根的生长受到抑制，幼苗生长减缓，种子的浸出液电解质含量升高。经EMS处理后，“中花”和“25号”芥蓝的生理指标表现较为一致，而“银绿”芥蓝的表现差异较显著，适宜诱变“银绿”芥蓝种子的半致死浓度为1.0%~1.5%。
- Abstract:
- Ｔhe seeds of three Chinese kale cultivated varieties of ‘Zhonghua’ ‘Yinlyu’‘Number 25’ were used as materials to explore the effects of different concentrations of EMS treatment on seed germination and seedling growth in order to determine the best dosage of EMS (ethyl methyl sulfonate) on Chinese kale induced mutation breeding.The results showed that with the increasing of EMS concentration,the germination rate and seedling survival rate decreased，growth of seedling roots was inhibited，and the leaching electrolyte content of seeds was elevated.The physiological indexes after treatment of EMS were consistent between ‘Zhonghua’ and ‘Number 25’，but significantly different from the varieties of ‘Yinlyu’.The optimum concentration of EMS treatment for Chinese kale ‘Yinlyu’ seeds was 1.0%-1.5% for 6 hours.
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备注/Memo
第一作者简介：吴思远（1993-），男，硕士研究生，研究方向为蔬菜遗传育种与生物技术。E-mail：1909046338@qq.com.责任作者：陈长明（1984-），男，博士，副教授，硕士生导师，研究方向为蔬菜遗传育种与生物技术。E-mail：cmchen@scau.edu.cn.基金项目：广东省科技计划资助项目（2015A020209117）；广州市自然科学基金资助项目（201804010320）；广东省自然科学基金资助项目（2017A030313096）。收稿日期：2017-12-20