[1]贺滉,牛美丽,党选民,等.外源物质对盐胁迫下西瓜种子发芽的影响[J].北方园艺,2018,42(04):66-71.[doi:10.11937/bfyy.20172646]
　HE Huang,NIU Meili,DANG Xuanmin,et al.Effects of Exogenous Substances on Germination of Watermelon Seeds Under Salt Stress[J].Northern Horticulture,2018,42(04):66-71.[doi:10.11937/bfyy.20172646]
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外源物质对盐胁迫下西瓜种子发芽的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 66-71 栏目: 出版日期: 2018-02-28
Title:
Effects of Exogenous Substances on Germination of Watermelon Seeds Under Salt Stress
作者:
贺滉1; 牛美丽2; 党选民1; 詹园凤1
1．中国热带农业科学院 热带作物品种资源研究所，海南 儋州 571737；2.舞阳县第一高级中学，河南 舞阳 462400
Author(s):
HE Huang1; NIU Meili2; DANG Xuanmin1; ZHAN Yuanfeng1
1.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737;2.First High School of Wuyang County,Wuyang,Henan 462400
关键词:
外源物质; 盐胁迫; 西瓜种子; 萌发
Keywords:
exogenous substances; salt stress; watermelon seeds; germination
DOI:
10.11937/bfyy.20172646
文献标志码:
A
摘要:
以小型西瓜品种“美月”种子为试材，在NaCl溶液中添加不同浓度的茉莉酸甲酯、氯化钙和脯氨酸，对其种子进行浸种处理，研究了外源物质〖JP2〗对西瓜种子发芽的影响。结果表明：130 mmol?L-1浓度NaCl处理效果最佳，以该浓度作为西瓜种子发芽处理盐浓度；茉莉酸甲酯、氯化钙和脯氨酸均能提高盐胁迫下种子发芽率、发芽势、发芽指数、活力指数以及根系活力、胚根长和胚轴长；以添加脯氨酸处理效果最佳，脯氨酸最适浓度为0.2 mmol?L-1，在此浓度下，发芽率和发芽势等指标显著提高。说明3种外源物质能够缓解盐胁迫对西瓜种子的伤害，促进西瓜种子的萌发。该研究为这3种外源物质在西瓜种植中的应用提供了依据。
Abstract:
Mini-watermelon variety of ‘Meiyue’ was used as test material,adding different concentrations of methyl jasmonate,calcium chloride and proline in NaCl solution,soaking seeds of ‘Meiyue’ watermelon,effects of exogenous substances on seed germination of watermelon were studied.The results indicated that the concentration of 130 mmol?L-1 NaCl was the best treatment concentration,could be used as the treatment concentration of watermelon seed germination;methyl jasmonate,calcium chloride and proline could increase the germination rate,germination potential,germination index,vigor index and root activity,root length and embryo length of seed under salt stress.Among them,the best effects were obtained by adding proline,the optimum concentration of proline was 0.2 mmol?L-1.At this concentration,the germination rate and germination potential were significantly improved.The results showed that three kinds of exogenous substances could alleviate the damage of watermelon seeds and promote the germination of watermelon seeds.This study provided the basis for the application of three exogenous substances in watermelon cultivation.

参考文献/References:

[1]杨建国,樊丽琴,许兴,等.盐碱地改良技术集成示范区水土环境变化研究初报[J].中国农学通报,2011,27（1):279-285．

[2]JI C J,YANG D C.Methyl jasmonate as vital substance in plants[J].Trends in Genetics,2003,19（7):409-413.
[3]HERRMANN G,LEHMANN J,PETERSON A,et al.Species and tissue specificity of jasmonate-induced abundant proteins[J].Journal of Plant Physiology,1989,134:703-709.
[4]陈培琴,郁松林,詹妍妮,等.茉莉酸对葡萄幼苗耐热性的影响[J].石河子大学学报,2006,24（10):87-91.
[5]李小玲,华智锐.外源茉莉酸甲酯对盐胁迫下黄芩种子萌发及幼苗生理特性的影响[J].山西农业科学,2016,44（11):1603-1607.
[6]AVALBAEV A,YULDASHEV R,FEDOROVA K,et al.Exogenous methyl jasmonate regulates cytokinin content by modulating cytokinin oxidase activity in wheat seedlings under salinity[J].J Plant Physiol,2016,191（4):101-110.
[7]ZHU J K.Regulation of ion homeostasis under salt stress[J].Curr Opin Plant Biol,2003（6):441-445.
[8]EPSTEIN E.How calcium enhances plant salt tolerance[J].Science,1998,280:1906-1917.
[9]RUS A,LEE B H,MAYOR A M,et al.AtHKT1 facilitates Na+ homeostasis and K+ nutrition in planta[J].Plant Physiol,2004,136:2500-2511.
[10]BRAAM J,DAVIS R W.Rain-wind and touch-induced expression of calmodulin and calmodulin related genes in Arabidopsis[J].Cell,1990,60:357-364.
[11]TUNA L,KAYA C,ASHRAF M,et al.The effects of calcium sulphate on growth,membrane stability and nutrient uptake of tomato plants grown under salt stress[J].Environmental and Experimental Botany,2007,59:173-178.
[12]陈晓云,刘洪庆,李发良,等.外源蔗糖和Ca2+对荞麦幼苗耐盐性的影响[J].植物生理学报,2012,48（12):1187-1192.
[13]〖JP2〗SRINIVAS V,BALASUBRAMANIAN D.Proline is a proteincompatible hydrotrope[J].Langmuir,1995,11（7):2830-2833．
[14]乔俊鹏,夏鹏云,梅海军,等.脯氨酸、维生素C和CaCl2处理对深山含笑抗寒性的影响[J].江西农业学报,2010,22（2):40-42.
[15]LSZL S,ARNOULD S.Proline:A multifunctional amino acid[J].Trends in Plant Science,2010,15（2):89-97.
[16]MATTIOLI R,MARCHESE D,D′ANGELI S,et al.Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis[J].Plant Mol Biol,2008,66（3):277-288.
[17]颜志明,孙锦,郭世荣.外源脯氨酸对NaCl胁迫下甜瓜幼苗生长和活性氧物质代谢的影响[J].江苏农业学报,2011,27（1):141-145.
[18]孙敏红,邓云,和相琼.NaCl胁迫对不同西瓜品种种子发芽及其幼苗生长特性的影响[J].北方园艺,2011（13):24-28.
[19]斯琴巴特尔,吴红英.盐胁迫对玉米种子萌发及幼苗生长的影响[J].干旱区资源与环境,2000,14（4):76-80.
[20]杨艺,常丹,王艳,等.盐胁迫下茉莉酸（JA)及茉莉酸甲酯（MeJA)对棉花种子萌发及种苗生化特性的影响[J].种子,2015,34（1):8-18.
[21]ANN M,EIS P,GUY B,et al.Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots[J].The Plant Cell,1997(9):2243-2259.

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备注/Memo

第一作者简介：贺滉（1981-），男，硕士，助理研究员，研究方向为蔬菜遗传育种。E-mail:hehuang_@163.com.

责任作者：詹园凤（1974-），女，硕士，副研究员，研究方向为蔬菜遗传育种。E-mail:zyf74216@163.com.
基金项目：中央级公益性科研院所基本科研业务费专项资助项目（1630032015016）；海南耕地改良关键技术研究与示范专项资助项目（HNGDhs2015）。
收稿日期：2017-10-30

更新日期/Last Update: 2018-02-24